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AB216482

Anti-Smad2 (phospho S465) antibody

4

(5 Reviews)

|

(8 Publications)

Rabbit Polyclonal SMAD2 phospho S465 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human SMAD2 phospho S465 aa 450 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

MADH2, MADR2, SMAD2, Mothers against decapentaplegic homolog 2, MAD homolog 2, Mothers against DPP homolog 2, JV18-1, Mad-related protein 2, SMAD family member 2, hMAD-2, SMAD 2, Smad2, hSMAD2

1 Images
Western blot - Anti-Smad2 (phospho S465) antibody (AB216482)
  • WB

Lab

Western blot - Anti-Smad2 (phospho S465) antibody (AB216482)

All lanes:

Western blot - Anti-Smad2 (phospho S465) antibody (ab216482) at 1/300 dilution

Lane 1:

mouse muscle lysates

Lane 2:

mouse heart lysates

Secondary

All lanes:

Conjugated secondary antibody at 1/10000 dilution

Predicted band size: 52 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human SMAD2 phospho S465 aa 450 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q15796

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/300", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/300", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.6 Preservative: 0.09% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Smad2 also known as Mothers against decapentaplegic homolog 2 (MAD2) or MADR2 is a signaling protein involved in the transforming growth factor-beta (TGF-β) receptor pathway. Smad2 has a molecular mass of approximately 58 kDa and expresses in various tissues including epithelial mesenchymal and endothelial cells. Smad2 undergoes phosphorylation on serine residues in response to TGF-β signaling converting it into phosphorylated forms often referred to as p-Smad2 or phospho-Smad2. These phosphorylated forms are critical for the relay of signals from the cell surface to the nucleus.
Biological function summary

Smad2 acts as an intracellular mediator for TGF-β signaling a pathway important for regulating cell proliferation differentiation and apoptosis. Smad2 typically functions as part of a heteromeric complex with Smad4 another key player in TGF-β signaling. Upon activation phosphorylated Smad2 combines with Smad4 to form a complex that translocates into the nucleus. This complex then binds to specific DNA sequences to regulate the transcription of target genes involved in processes such as cell growth inhibition and extracellular matrix production.

Pathways

Smad2 is integral to the TGF-β and activin receptor signaling pathways. These pathways are essential in controlling cell growth and immune responses. Smad2 interacts with other proteins such as Smad3 in addition to Smad4 to modulate gene expression effectively. The interaction between Smad2 and these proteins ensures precise control of cellular responses to external stimuli emphasizing its pivotal role in maintaining cellular homeostasis.

Smad2 correlates with various pathological conditions including fibrosis and cancer. Aberrant Smad2 signaling can contribute to the development of these diseases as excessive TGF-β signaling promotes fibrotic tissue deposition and tumor progression. Smad2 connects with other proteins like Smad3 in these pathological contexts both acting as mediators of abnormal cellular behaviors. Understanding the regulatory mechanisms of Smad2 can help develop therapeutic strategies against disorders linked to dysregulated TGF-β signaling.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. Promotes TGFB1-mediated transcription of odontoblastic differentiation genes in dental papilla cells (By similarity). Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. May act as a tumor suppressor in colorectal carcinoma (PubMed : 8752209).
See full target information SMAD2 phospho S465
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:529 PubMed40664638

2025

Modeling oxaliplatin resistance in colorectal cancer reveals a SERPINE1-based gene signature (RESIST-M) and therapeutic strategies for pro-metastatic CMS4 subtype.

Applications

Unspecified application

Species

Unspecified reactive species

Stephen Qi Rong Wong,Mohua Das,Kenzom Tenzin,Niranjan Shirgaonkar,Huiwen Chua,Lin Xuan Chee,Ahpa Sae Yeoh,Astley Aruna Murugiah,Wei Yong Chua,Madelaine Skolastika Theardy,Ethan Jadon Subel,Matan Thangavelu Thangavelu,Jane Vin Chan,Choon Kong Yap,Iain Bee Huat Tan,Petros Tsantoulis,Sabine Tejpar,Jia Min Loo,Ramanuj DasGupta

Cellular and molecular life sciences : CMLS 81:187 PubMed38635081

2024

Role of transient receptor potential ankyrin 1 in idiopathic pulmonary fibrosis: modulation of M2 macrophage polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Yang,Zhenyu Xiao,Weijie Yang,Yangyang Sun,Xin Sui,Xueyang Lin,Xinyi Yang,Zhenghao Bao,Ziqi Cui,Yingkai Ma,Weidong Li,Shengran Wang,Jun Yang,Yongan Wang,Yuan Luo

Cancer cell 41:1911-1926.e8 PubMed37802053

2023

TIM-3 blockade in diffuse intrinsic pontine glioma models promotes tumor regression and antitumor immune memory.

Applications

Unspecified application

Species

Unspecified reactive species

Iker Ausejo-Mauleon,Sara Labiano,Daniel de la Nava,Virginia Laspidea,Marta Zalacain,Lucía Marrodán,Marc García-Moure,Marisol González-Huarriz,Irati Hervás-Corpión,Laasya Dhandapani,Silvestre Vicent,Maria Collantes,Iván Peñuelas,Oren J Becher,Mariella G Filbin,Li Jiang,Jenna Labelle,Carlos A O de Biagi-Junior,Javad Nazarian,Sandra Laternser,Timothy N Phoenix,Jasper van der Lugt,Mariette Kranendonk,Raoull Hoogendijk,Sabine Mueller,Carlos De Andrea,Ana C Anderson,Elizabeth Guruceaga,Carl Koschmann,Viveka Nand Yadav,Jaime Gállego Pérez-Larraya,Ana Patiño-García,Fernando Pastor,Marta M Alonso

Pharmacology 108:432-443 PubMed37343534

2023

Quercetin Alleviates Asthma-Induced Airway Inflammation and Remodeling through Downregulating Periostin via Blocking TGF-β1/Smad Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yanni Fang,Wenwen Jin,Zhen Guo,Jumei Hao

Scientific reports 11:14088 PubMed34239012

2021

Similarities and differences between IL11 and IL11RA1 knockout mice for lung fibro-inflammation, fertility and craniosynostosis.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin Ng,Anissa A Widjaja,Sivakumar Viswanathan,Jinrui Dong,Sonia P Chothani,Stella Lim,Shamini G Shekeran,Jessie Tan,Narelle E McGregor,Emma C Walker,Natalie A Sims,Sebastian Schafer,Stuart A Cook

Advances in clinical and experimental medicine : official organ Wroclaw Medical University 30:395-403 PubMed33857357

2021

LncRNA NORAD regulates scar hypertrophy via miRNA-26a mediating the regulation of TGFβR1/2.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Qi,Yangyang Wu,Haijian Zhang,Yifei Liu

Medical science monitor : international medical jo 26:e922029 PubMed32447340

2020

Long Non-Coding RNA (LncRNA)-ATB Promotes Inflammation, Cell Apoptosis and Senescence in Transforming Growth Factor-β1 (TGF-β1) Induced Human Kidney 2 (HK-2) Cells via TGFβ/SMAD2/3 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Han Sun,Cong Ke,Lin Zhang,Changjun Tian,Zhihui Zhang,Shuhua Wu

FASEB journal : official publication of the Federa 33:8809-8821 PubMed31042406

2019

Characterization of articular cartilage homeostasis and the mechanism of superior cartilage regeneration of MRL/MpJ mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenhan Deng,Xueqin Gao,Xuying Sun,Sarah Amra,Aiping Lu,Yan Cui,Holger K Eltzschig,Guanghua Lei,Johnny Huard
View all publications
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