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AB326682

Anti-Smad2 (phospho S465 + S467) antibody [SR4368]

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Rabbit Recombinant Monoclonal SMAD2 phospho S465 + S467 antibody. Suitable for WB, ICC/IF and reacts with Human, Rat, Mouse samples.

View Alternative Names

MADH2, MADR2, SMAD2, SMAD family member 2, SMAD 2, hSMAD2, JV18-1, Mad-related protein 2, Mothers against decapentaplegic homolog 2, hMAD-2, MAD homolog 2, Mothers against DPP homolog 2

1 Images
Western blot - Anti-Smad2 (phospho S465 + S467) antibody [SR4368] (AB326682)
  • WB

Supplier Data

Western blot - Anti-Smad2 (phospho S465 + S467) antibody [SR4368] (AB326682)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : Hela whole cell lysates
Lane 2 : Hela treated with 10ng/ml TGF-β3 for 20 minutes whole cell lysates
Lane 3 : 3T3 whole cell lysates
Lane 4 : 3T3 treated with 10ng/ml TGF-β3 for 20 minutes whole cell lysates
Lane 5 : C6 whole cell lysates
Lane 6 : C6 treated with 10ng/ml TGF-β3 for 20 minutes whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size : 52 kDa
Observed band size : 60 kDa

Exposure time : 6 seconds

All lanes:

Western blot - Anti-Smad2 (phospho S465 + S467) antibody [SR4368] (ab326682) at 1/1000 dilution

Lane 1:

Hela whole cell lysates at 20 µg

Lane 2:

Hela treated with 10ng/ml TGF-β3 for 20 minutes whole cell lysates at 20 µg

Lane 3:

3T3 whole cell lysates at 20 µg

Lane 4:

3T3 treated with 10ng/ml TGF-β3 for 20 minutes whole cell lysates at 20 µg

Lane 5:

C6 whole cell lysates at 20 µg

Lane 6:

C6 treated with 10ng/ml TGF-β3 for 20 minutes whole cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 52 kDa

Observed band size: 60 kDa

false

Exposure time: 6s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SR4368

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.05% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. Promotes TGFB1-mediated transcription of odontoblastic differentiation genes in dental papilla cells (By similarity). Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. May act as a tumor suppressor in colorectal carcinoma (PubMed : 8752209).
See full target information SMAD2 pS465 + S467
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com