AB61066

Anti-Smad2 (phospho T220) antibody

Name: Anti-Smad2 (phospho T220) antibody (ab61066) | Abcam
Brand: Abcam Limited
SKU: AB61066
Price: 535 USD
Availability: InStock

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Polyclonal SMAD2 phospho T220 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SMAD2 phospho T220 aa 150-250.

View Alternative Names

MADH2, MADR2, SMAD2, Mothers against decapentaplegic homolog 2, MAD homolog 2, Mothers against DPP homolog 2, JV18-1, Mad-related protein 2, SMAD family member 2, hMAD-2, SMAD 2, Smad2, hSMAD2

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-Smad2 (phospho T220) antibody (AB61066)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Smad2 (phospho T220) antibody (AB61066)

Immunofluorescence analysis of HeLa cells, using ab61066 antibody at 1/500-1/1000 dilution with or without immunizing peptide.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Synthetic Peptide within Human SMAD2 phospho T220 aa 150-250. The exact immunogen used to generate this antibody is proprietary information.

Q15796

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Smad2 also known as Mothers against decapentaplegic homolog 2 (MAD2) or MADR2 is a signaling protein involved in the transforming growth factor-beta (TGF-β) receptor pathway. Smad2 has a molecular mass of approximately 58 kDa and expresses in various tissues including epithelial mesenchymal and endothelial cells. Smad2 undergoes phosphorylation on serine residues in response to TGF-β signaling converting it into phosphorylated forms often referred to as p-Smad2 or phospho-Smad2. These phosphorylated forms are critical for the relay of signals from the cell surface to the nucleus.

Biological function summary

Smad2 acts as an intracellular mediator for TGF-β signaling a pathway important for regulating cell proliferation differentiation and apoptosis. Smad2 typically functions as part of a heteromeric complex with Smad4 another key player in TGF-β signaling. Upon activation phosphorylated Smad2 combines with Smad4 to form a complex that translocates into the nucleus. This complex then binds to specific DNA sequences to regulate the transcription of target genes involved in processes such as cell growth inhibition and extracellular matrix production.

Pathways

Smad2 is integral to the TGF-β and activin receptor signaling pathways. These pathways are essential in controlling cell growth and immune responses. Smad2 interacts with other proteins such as Smad3 in addition to Smad4 to modulate gene expression effectively. The interaction between Smad2 and these proteins ensures precise control of cellular responses to external stimuli emphasizing its pivotal role in maintaining cellular homeostasis.

Smad2 correlates with various pathological conditions including fibrosis and cancer. Aberrant Smad2 signaling can contribute to the development of these diseases as excessive TGF-β signaling promotes fibrotic tissue deposition and tumor progression. Smad2 connects with other proteins like Smad3 in these pathological contexts both acting as mediators of abnormal cellular behaviors. Understanding the regulatory mechanisms of Smad2 can help develop therapeutic strategies against disorders linked to dysregulated TGF-β signaling.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. Promotes TGFB1-mediated transcription of odontoblastic differentiation genes in dental papilla cells (By similarity). Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. May act as a tumor suppressor in colorectal carcinoma (PubMed : 8752209).

See full target information SMAD2 phospho T220

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Science advances 10:eadk8908 PubMed38781342

2024

CK2-dependent degradation of CBX3 dictates replication fork stalling and PARP inhibitor sensitivity.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Ma,Dianyun Ren,Zixi Wang,Wei Li,Lei Li,Tianjie Liu,Qi Ye,Yuzeshi Lei,Yanlin Jian,Bohan Ma,Yizeng Fan,Jing Liu,Yang Gao,Xin Jin,Haojie Huang,Lei Li

Journal of the American Society of Nephrology : JASN 34:809-828 PubMed36724799

2023

Hyperactivation of YAP/TAZ Drives Alterations in Mesangial Cells through Stabilization of N-Myc in Diabetic Nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Seunghyeok Choi,Seon Pyo Hong,Jung Hyun Bae,Sang Heon Suh,Hosung Bae,Kyung Pyo Kang,Hyuek Jong Lee,Gou Young Koh

View all publications

style

left-column

style

left-column

style

right-column

Alternative Products

Primary Antibodies

AB196538

Alexa Fluor® 647 Anti-Smad2 antibody [EP567Y]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Smad2 antibody [EP567Y] (AB196538)

Primary Antibodies

AB184557

Anti-Smad2 (phospho S250) antibody [EPR2855(2)]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Smad2 (phospho S250) antibody [EPR2855(2)] (AB184557)

Primary Antibodies

AB33875

Anti-Smad2 antibody [EP567Y]

20ul selling size|RabMAb|Advanced Validation|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Smad2 antibody [EP567Y] (AB33875)

Primary Antibodies

AB219598

Anti-Smad2 (phospho S255) antibody [EPR2856(N)] - BSA and Azide free

Recombinant|Advanced Validation|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad2 (phospho S255) antibody [EPR2856(N)] - BSA and Azide free (AB219598)

Primary Antibodies

AB40855

Anti-Smad2 antibody [EP784Y]

20ul selling size|RabMAb|Advanced Validation|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Smad2 antibody [EP784Y] (AB40855)

Primary Antibodies

AB188334

Anti-Smad2 (phospho S255) antibody [EPR2856(N)]

20ul selling size|Recombinant|Advanced Validation|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad2 (phospho S255) antibody [EPR2856(N)] (AB188334)

Primary Antibodies

AB157371

Anti-Smad2 antibody [EP784Y] - BSA and Azide free

RabMAb|Advanced Validation|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Smad2 antibody [EP784Y] - BSA and Azide free (AB157371)

Complementary Products

Primary Antibodies

AB52903

Anti-SMAD3 (pS423/425) + SMAD5 (pS463/465 ) + SMAD1 (pS463/465) + SMAD2 (pS465/467) antibody [EP823Y]

20ul selling size|Recombinant|Advanced Validation|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMAD3 (pS423/425) + SMAD5 (pS463/465 ) + SMAD1 (pS463/465) + SMAD2 (pS465/467) antibody [EP823Y] (AB52903)

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Primary Antibodies

AB215715

Anti-TGF beta 1 antibody [EPR21143]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-TGF beta 1 antibody [EPR21143] (AB215715)

Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com