Anti-Smad2 + Smad3 antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Smad2 + Smad3 antibody (AB217553)

Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Smad3) polyclonal Antibody, Unconjugated (ab217553) 1 : 100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

• Flow Cyt

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Flow Cytometry - Anti-Smad2 + Smad3 antibody (AB217553)

HUVEC cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Smad2/3 Polyclonal Antibody(ab217553) at 1 : 50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

• WB

Supplier Data

Western blot - Anti-Smad2 + Smad3 antibody (AB217553)

12% SDS-PAGE

All lanes:

Western blot - Anti-Smad2 + Smad3 antibody (ab217553) at 1/200 dilution

Lane 1:

mouse brain lysates

Lane 2:

mouse kidney lysates

Secondary

All lanes:

HRP-conjugated secondary antibody at 1/3000 dilution

Observed band size: 47 kDa

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• WB

Supplier Data

Western blot - Anti-Smad2 + Smad3 antibody (AB217553)

All lanes:

Western blot - Anti-Smad2 + Smad3 antibody (ab217553) at 1/1000 dilution

Lane 1:

Mouse cerebrum lysate

Lane 2:

Mouse heart lysate

Lane 3:

Mouse testis lysate

Lane 4:

Mouse Skin lysate

Lane 5:

Mouse Kidney lysate

Lane 6:

Rat cerebrum lysate

Lane 7:

Rat Testis lysate

Lane 8:

Rat kidney lysate

Lane 9:

HUVEC cell lysate

Lane 10:

A459 cell lysate

Lane 11:

HeLa cell lysate

Lane 12:

HT1080 cell lysate

Lane 13:

A431 cell lysate

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