AB63672

Anti-Smad2 + Smad3 antibody

Name: Anti-Smad2 + Smad3 antibody (ab63672) | Abcam
Brand: Abcam Limited
SKU: AB63672
Price: 525 USD
Availability: InStock

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(19 Publications)

Rabbit Polyclonal SMAD2 antibody. Suitable for WB and reacts with African green monkey, Human samples. Cited in 19 publications. Immunogen corresponding to Synthetic Peptide within Human SMAD2.

View Alternative Names

MADH2, MADR2, SMAD2, Mothers against decapentaplegic homolog 2, MAD homolog 2, Mothers against DPP homolog 2, JV18-1, Mad-related protein 2, SMAD family member 2, hMAD-2, SMAD 2, Smad2, hSMAD2

1 Images

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Western blot - Anti-Smad2 + Smad3 antibody (AB63672)

All lanes:

Western blot - Anti-Smad2 + Smad3 antibody (ab63672) at 1/500 dilution

Lane 1:

COS-7 (African green monkey kidney fibroblast-like cell line) cell extracts treated with UV (15mins) at 30 µg

Lane 2:

COS-7 (African green monkey kidney fibroblast-like cell line) cell extracts untreated at 30 µg

Lane 3:

Jurkat cell extracts treated with EGF (200ng/ml, 30mins) at 30 µg

Lane 4:

Jurkat cell extracts treated with EGF (200ng/ml, 30mins) at 30 µg with immunizing peptide

Observed band size: 48 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, African green monkey

Applications

applications

Immunogen

Synthetic Peptide within Human SMAD2. The exact immunogen used to generate this antibody is proprietary information.

Q15796

Specificity

ab63672 detects endogenous levels of total Smad2 and Smad3.

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Smad2 and Smad3 are intracellular proteins tightly involved in the TGF-β signaling pathway. These proteins also known as mothers against decapentaplegic homolog 2 and 3 play an important role in transmitting signals from the cell surface to the nucleus. Smad2 has a molecular weight of approximately 58 kDa while Smad3 is about 52 kDa. They are expressed in various tissues throughout the body including heart skin and immune cells. Upon activation Smad2 and Smad3 undergo phosphorylation a modification that is important for their function as signal transducers.

Biological function summary

The functions of Smad2 and Smad3 are critical in mediating cellular processes such as proliferation differentiation and apoptosis. These proteins often form complexes specifically oligomeric structures with other Smad proteins like co-Smad4 to exert their regulatory roles. The Smad2/3 complexes control the transcription of specific genes in response to TGF-β stimuli influencing cellular responses in a context-dependent manner.

Pathways

Smad2 and Smad3 are central to the TGF-β signaling pathway one significant pathway that affects cellular growth and differentiation. These proteins interact with TGF-β receptors on the cell surface to become phosphorylated forming a complex with Smad4 which then translocates to the nucleus. In the nucleus this complex regulates gene expression. Both Smad2 and Smad3 also engage with other signaling pathways such as the MAPK pathway further influencing cell behavior and cooperating with proteins like ERK.

Smad2 and Smad3 have been linked to conditions like cancer and fibrosis. Dysregulation of Smad2/3 signaling often leads to unchecked cell proliferation a hallmark of tumorigenesis. In fibrosis excessive Smad2/3 activity contributes to tissue scarring due to increased extracellular matrix production. For instance their interaction with proteins like α-SMA (alpha-smooth muscle actin) in fibroblasts plays a role in cardiac and pulmonary fibrosis. Understanding their exact contribution provides insights into potential therapeutic targets for treating these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. Promotes TGFB1-mediated transcription of odontoblastic differentiation genes in dental papilla cells (By similarity). Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. May act as a tumor suppressor in colorectal carcinoma (PubMed : 8752209).

See full target information SMAD2

Additional targets

SMAD3

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 21:778 PubMed37919785

2023

LncRNA-Gm9866 promotes liver fibrosis by activating TGFβ/Smad signaling via targeting Fam98b.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaomin Liao,Xianxian Ruan,Peishan Yao,Dan Yang,Xianbin Wu,Xia Zhou,Jie Jing,Dafu Wei,Yaodan Liang,Taicheng Zhang,Shanyu Qin,Haixing Jiang

Arthritis & rheumatology (Hoboken, N.J.) 74:860-870 PubMed34882985

2022

Therapeutic Effect of Cyclin-Dependent Kinase 4/6 Inhibitor on Dermal Fibrosis in Murine Models of Systemic Sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Akio Yamamoto,Tetsuya Saito,Tadashi Hosoya,Kimito Kawahata,Yoshihide Asano,Shinichi Sato,Fumitaka Mizoguchi,Shinsuke Yasuda,Hitoshi Kohsaka

Oncogene 41:1780-1794 PubMed35132181

2022

Active demethylation upregulates CD147 expression promoting non-small cell lung cancer invasion and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng-Gong Liao,Xiao-Hua Liang,Yuan Ke,Li Yao,Man Liu,Ze-Kun Liu,Lin He,Yi-Xiao Guo,Huijie Bian,Zhi-Nan Chen,Ling-Min Kong

Experimental and therapeutic medicine 20:117 PubMed33005243

2020

Irbesartan ameliorates myocardial fibrosis in diabetic cardiomyopathy rats by inhibiting the TGFβ1/Smad2/3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Min Zong,Hua Zhao,Qiang Li,Yanbing Li,Jianjun Zhang

Stem cell research & therapy 11:365 PubMed32831139

2020

A novel tsRNA-16902 regulating the adipogenic differentiation of human bone marrow mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Wang,Jun Mei,Xingnuan Li,Xiaoyuan Xu,Baicheng Ma,Weidong Li

American journal of physiology. Cell physiology 319:C392-C401 PubMed32348176

2020

TRIM59 inhibits PPM1A through ubiquitination and activates TGF-β/Smad signaling to promote the invasion of ectopic endometrial stromal cells in endometriosis.

Applications

Unspecified application

Species

Unspecified reactive species

Fengyu Wang,Haili Wang,Lei Sun,Chengling Niu,Jie Xu

BioMed research international 2020:3451215 PubMed32258113

2020

Isoflurane Postconditioning Upregulates Phosphorylated Connexin 43 in the Middle Cerebral Artery Occlusion Model and Is Probably Associated with the TGF-1/Smad2/3 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jiangwen Yin,Xuejiao Liu,Ruixue Wang,Mingyue Ge,Liping Xie,Jingwen Zhai,Zhigang Dai,Yan Li,Sheng Wang

IUBMB life 71:1382-1390 PubMed31087761

2019

Downregulation of hedgehog ligands in human simple steatosis may protect against nonalcoholic steatohepatitis: Is TAZ a crucial regulator?

Applications

Unspecified application

Species

Unspecified reactive species

Zohreh Khajehahmadi,Sina Mohagheghi,Saman Nikeghbalian,Bita Geramizadeh,Iraj Khodadadi,Jamshid Karimi,Mohammad Ebrahim Ghaffari,Heidar Tavilani

Regenerative therapy 11:17-24 PubMed31193169

2019

Asperosaponin VI stimulates osteogenic differentiation of rat adipose-derived stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xingpo Ding,Wuyin Li,Dengshan Chen,Chuanwei Zhang,Lei Wang,Hong Zhang,Na Qin,Yongqiang Sun

Evidence-based complementary and alternative medic 2019:5813549 PubMed30713574

2019

Effect of Kangxianling Decoction on Expression of TGF-1/Smads and Extracellular Matrix Deposition.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Ji,Liqun He

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Smad2 + Smad3 antibody

Western blot - Anti-Smad2 + Smad3 antibody (AB63672)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (19)

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Product promise