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Rabbit Recombinant Monoclonal SMAD3 antibody. Carrier free. Suitable for ChIC/CUT&RUN-seq, WB, IP, ChIP, ICC/IF, Flow Cyt (Intra) and reacts with Human, Recombinant fragment - Human samples. Cited in 1 publication.

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Images

Immunoprecipitation - Anti-Smad2 + Smad3 antibody [EPR19557] - BSA and Azide free (AB236030), expandable thumbnail
  • ChIP - Anti-Smad2 + Smad3 antibody [EPR19557] - BSA and Azide free (AB236030), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Smad2 + Smad3 antibody [EPR19557] - BSA and Azide free (AB236030), expandable thumbnail
  • Immunoprecipitation - Anti-Smad2 + Smad3 antibody [EPR19557] - BSA and Azide free (AB236030), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Smad2 + Smad3 antibody [EPR19557] - BSA and Azide free (AB236030), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ChIC/CUT&RUN-seqWBIPChIPICC/IFFlow Cyt (Intra)
Human
Tested
Expected
Tested
Tested
Tested
Tested
Recombinant fragment - Human
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

SMAD3 is a receptor-regulated SMAD that functions as an intracellular signal transducer and transcriptional modulator, activated by TGF-beta and activin type 1 receptor kinases. It binds to the TRE element in promoters of numerous genes regulated by TGF-beta, and upon forming a complex with SMAD4, activates transcription. Additionally, SMAD3 can form a complex with SMAD4, JUN, and FOS at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. SMAD3 may inhibit wound healing by modulating the growth and migration of primary keratinocytes and altering TGF-beta-mediated monocyte chemotaxis, with this effect being potentially hormone-sensitive. Furthermore, SMAD3 is involved in regulating chondrogenesis and osteogenesis and may inhibit early bone fracture healing. It also positively regulates PDPK1 kinase activity by promoting its dissociation from the 14-3-3 protein YWHAQ, which negatively regulates it. This supplementary information is collated from multiple sources and compiled automatically.

Additional Targets

SMAD2

Alternative names

Recommended products

Rabbit Recombinant Monoclonal SMAD3 antibody. Carrier free. Suitable for ChIC/CUT&RUN-seq, WB, IP, ChIP, ICC/IF, Flow Cyt (Intra) and reacts with Human, Recombinant fragment - Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR19557
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab236030 is the carrier-free version of Anti-Smad2 + Smad3 antibody [EPR19557] - ChIP Grade ab207447.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Smad2 and Smad3 are intracellular proteins tightly involved in the TGF-β signaling pathway. These proteins also known as mothers against decapentaplegic homolog 2 and 3 play an important role in transmitting signals from the cell surface to the nucleus. Smad2 has a molecular weight of approximately 58 kDa while Smad3 is about 52 kDa. They are expressed in various tissues throughout the body including heart skin and immune cells. Upon activation Smad2 and Smad3 undergo phosphorylation a modification that is important for their function as signal transducers.

Biological function summary

The functions of Smad2 and Smad3 are critical in mediating cellular processes such as proliferation differentiation and apoptosis. These proteins often form complexes specifically oligomeric structures with other Smad proteins like co-Smad4 to exert their regulatory roles. The Smad2/3 complexes control the transcription of specific genes in response to TGF-β stimuli influencing cellular responses in a context-dependent manner.

Pathways

Smad2 and Smad3 are central to the TGF-β signaling pathway one significant pathway that affects cellular growth and differentiation. These proteins interact with TGF-β receptors on the cell surface to become phosphorylated forming a complex with Smad4 which then translocates to the nucleus. In the nucleus this complex regulates gene expression. Both Smad2 and Smad3 also engage with other signaling pathways such as the MAPK pathway further influencing cell behavior and cooperating with proteins like ERK.

Associated diseases and disorders

Smad2 and Smad3 have been linked to conditions like cancer and fibrosis. Dysregulation of Smad2/3 signaling often leads to unchecked cell proliferation a hallmark of tumorigenesis. In fibrosis excessive Smad2/3 activity contributes to tissue scarring due to increased extracellular matrix production. For instance their interaction with proteins like α-SMA (alpha-smooth muscle actin) in fibroblasts plays a role in cardiac and pulmonary fibrosis. Understanding their exact contribution provides insights into potential therapeutic targets for treating these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com