AB272332

Anti-Smad2 + Smad3 (phospho T8) antibody

Name: Anti-Smad2 + Smad3 (phospho T8) antibody (ab272332) | Abcam
Brand: Abcam Limited
SKU: AB272332
Price: 425 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(30 Publications)

Rabbit Polyclonal SMAD3 phospho T8 antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 30 publications. Immunogen corresponding to Synthetic Peptide within Human SMAD3 phospho T8.

View Alternative Names

MADH3, SMAD3, Mothers against decapentaplegic homolog 3, MAD homolog 3, Mad3, Mothers against DPP homolog 3, hMAD-3, JV15-2, SMAD family member 3, SMAD 3, Smad3, hSMAD3

1 Images

Supplier Data

Western blot - Anti-Smad2 + Smad3 (phospho T8) antibody (AB272332)

All lanes:

Western blot - Anti-Smad2 + Smad3 (phospho T8) antibody (ab272332) at 1/500 dilution

Lane 1:

HEK293T whole cell lysate

Lane 2:

HEK293T treated with TNFα(20ng/ml)for 10 min whole cell lysate

Lane 3:

Mouse Heart tissue lysate

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Synthetic Peptide within Human SMAD3 phospho T8. The exact immunogen used to generate this antibody is proprietary information.

P84022

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Smad2 and Smad3 are intracellular proteins tightly involved in the TGF-β signaling pathway. These proteins also known as mothers against decapentaplegic homolog 2 and 3 play an important role in transmitting signals from the cell surface to the nucleus. Smad2 has a molecular weight of approximately 58 kDa while Smad3 is about 52 kDa. They are expressed in various tissues throughout the body including heart skin and immune cells. Upon activation Smad2 and Smad3 undergo phosphorylation a modification that is important for their function as signal transducers.

Biological function summary

The functions of Smad2 and Smad3 are critical in mediating cellular processes such as proliferation differentiation and apoptosis. These proteins often form complexes specifically oligomeric structures with other Smad proteins like co-Smad4 to exert their regulatory roles. The Smad2/3 complexes control the transcription of specific genes in response to TGF-β stimuli influencing cellular responses in a context-dependent manner.

Pathways

Smad2 and Smad3 are central to the TGF-β signaling pathway one significant pathway that affects cellular growth and differentiation. These proteins interact with TGF-β receptors on the cell surface to become phosphorylated forming a complex with Smad4 which then translocates to the nucleus. In the nucleus this complex regulates gene expression. Both Smad2 and Smad3 also engage with other signaling pathways such as the MAPK pathway further influencing cell behavior and cooperating with proteins like ERK.

Smad2 and Smad3 have been linked to conditions like cancer and fibrosis. Dysregulation of Smad2/3 signaling often leads to unchecked cell proliferation a hallmark of tumorigenesis. In fibrosis excessive Smad2/3 activity contributes to tissue scarring due to increased extracellular matrix production. For instance their interaction with proteins like α-SMA (alpha-smooth muscle actin) in fibroblasts plays a role in cardiac and pulmonary fibrosis. Understanding their exact contribution provides insights into potential therapeutic targets for treating these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

SMAD3 is a receptor-regulated SMAD that functions as an intracellular signal transducer and transcriptional modulator, activated by TGF-beta and activin type 1 receptor kinases. It binds to the TRE element in promoters of numerous genes regulated by TGF-beta, and upon forming a complex with SMAD4, activates transcription. Additionally, SMAD3 can form a complex with SMAD4, JUN, and FOS at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. SMAD3 may inhibit wound healing by modulating the growth and migration of primary keratinocytes and altering TGF-beta-mediated monocyte chemotaxis, with this effect being potentially hormone-sensitive. Furthermore, SMAD3 is involved in regulating chondrogenesis and osteogenesis and may inhibit early bone fracture healing. It also positively regulates PDPK1 kinase activity by promoting its dissociation from the 14-3-3 protein YWHAQ, which negatively regulates it. This supplementary information is collated from multiple sources and compiled automatically.

See full target information SMAD3 phospho T8

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Publications (30)

Recent publications for all applications. Explore the full list and refine your search

Journal of thoracic disease 17:4249-4263 PubMed40688308

2025

regulation of cardiac fibroblast proliferation via the TGF-β/Smad pathway in an model of atrial fibrillation.

Applications

Unspecified application

Species

Unspecified reactive species

Yimeng Zhou,Lianzhi Zhang,Wenjia Zhang,Qunlin Gong,Zhong Zhang,Nan Xu,Ashley R Wilson-Smith,Nannan Chen

American journal of reproductive immunology (New York, N.Y. : 1989) 93:e70102 PubMed40435048

2025

Knockdown of TRIM47 Overcomes Paclitaxel Resistance in Ovarian Cancer by Suppressing the TGF-β Pathway via PPM1A.

Applications

Unspecified application

Species

Unspecified reactive species

Hezhu Wang,Shengjun Chen,Feifei Xu,Xiaojing Chen

Theranostics 14:6185-6201 PubMed39431015

2024

Spatial transcriptomic sequencing reveals immune microenvironment features of granulomas in lung and omentum.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaochen Qiu,Pengfei Zhong,Liang Yue,Chaofan Li,Zhimin Yun,Guangqian Si,Mengfan Li,Zhi Chen,Yingxia Tan,Pengtao Bao

Materials today. Bio 28:101162 PubMed39175654

2024

Type I collagen-targeted liposome delivery of Serca2a modulates myocardium calcium homeostasis and reduces cardiac fibrosis induced by myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Wanshi Chen,Lingjuan Liu,Ming Tang,Jiajin Li,Wenjing Yuan,Dan Yin,Yang Cao,Jie Tian

Photochemistry and photobiology 100:1874-1893 PubMed38504445

2024

Compound collagen peptide powder improves skin photoaging by reducing oxidative stress and activating TGF-β1/Smad pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kaien Guo,Linxin Zheng,Xin Zeng,Guangchun Huang,Lingling Meng,Yuting Yin

CNS neuroscience & therapeutics 30:e14630 PubMed38348765

2024

TREM2 gene induces differentiation of induced pluripotent stem cells into dopaminergic neurons and promotes neuronal repair via TGF-β activation in 6-OHDA-lesioned mouse model of Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Hanbai Liang,Ping Liu,Zijing Wang,Huan Xiong,Cheng Yin,Dongdong Zhao,Chunhui Wu,Longyi Chen

Journal of diabetes research 2024:6942156 PubMed38282657

2024

Huaju Xiaoji Formula Regulates ERS-lncMGC/miRNA to Enhance the Renal Function of Hypertensive Diabetic Mice with Nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Zeng Zhang,Xiaodong Fu,Fengzhu Zhou,Duanchun Zhang,Yanqiu Xu,Zhaohua Fan,Shimei Wen,Yanting Shao,Zheng Yao,Yanming He

British journal of pharmacology 181:1308-1323 PubMed37990806

2023

Paediatric sepsis survivors are resistant to sepsis-induced long-term immune dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

David F Colón,Carlos W Wanderley,Walter M Turato,Vanessa F Borges,Marcelo Franchin,Fernanda V S Castanheira,Daniele Nascimento,Douglas Prado,Mikhael Haruo Fernandes de Lima,Leila C Volpon,Silvia K Kavaguti,Ana P Carlotti,Fabio Carmona,Bernardo S Franklin,Thiago M Cunha,Jose Carlos Alves-Filho,Fernando Q Cunha

Aging 15:11985-11993 PubMed37910782

2023

Inhibition of keloid by P isotope radiotherapy through suppressing TGF-β/Smad signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Long Xie,Liqun Huang,Guanjie Zhang,Yingrui Su

Journal of neuroinflammation 20:175 PubMed37507781

2023

Smad7 in the hippocampus contributes to memory impairment in aged mice after anesthesia and surgery.

Applications

Unspecified application

Species

Unspecified reactive species

Changliang Liu,Jiahui Wu,Ming Li,Rui Gao,Xueying Zhang,Shixin Ye-Lehmann,Jiangning Song,Tao Zhu,Chan Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Smad2 + Smad3 (phospho T8) antibody

Western blot - Anti-Smad2 + Smad3 (phospho T8) antibody (AB272332)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (30)

Complementary Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise