Rabbit Polyclonal SMAD3 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 186 publications.
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- Cells 12:2023Sertoli Cell-Specific Activation of Transforming Growth Factor Beta Receptor 1 Leads to Testicular Granulosa Cell Tumor Formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Fang,Linfeng Nie,Satwikreddy Putluri,Nan Ni,Laurent Bartholin,Qinglei LiPubMed 38067144
- International journal of molecular sciences 24:2023Smad3 Mediates Diabetic Dyslipidemia and Fatty Liver in db/db Mice by Targeting PPARδ.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuijun He,Yu Zhong,Honglian Wang,Patrick Ming-Kuen Tang,Vivian Weiwen Xue,Xiaocui Chen,Jiaoyi Chen,Xiaoru Huang,Cheng Wang,Huiyao LanPubMed 37511155
- Cell discovery 9:412023PHF14 enhances DNA methylation of SMAD7 gene to promote TGF-β-driven lung adenocarcinoma metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHan Tian,Chenying Liu,Jianchen Yu,Jian Han,Jianan Du,Shujun Liang,Wenting Wang,Qin Liu,Rong Lian,Ting Zhu,Shanshan Wu,Tianyu Tao,Yaokai Ye,Jingjing Zhao,Yi Yang,Xun Zhu,Junchao Cai,Jueheng Wu,Mengfeng LiPubMed 37072414
- Nucleic acids research 51:2655-26702023SMAD3 promotes expression and activity of the androgen receptor in prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHee-Young Jeon,Majid Pornour,Hyunju Ryu,Sudeep Khadka,Rui Xu,Jihyun Jang,Deqiang Li,Hegang Chen,Arif Hussain,Ladan Fazli,Martin Gleave,Xuesen Dong,Furong Huang,Qianben Wang,Christopher Barbieri,Jianfei QiPubMed 36727462
- Communications biology 5:13012022Single-cell multiomics reveals the complexity of TGFβ signalling to chromatin in iPSC-derived kidney organoids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJessica L Davis,Ciaran Kennedy,Shane Clerkin,Niall J Treacy,Thomas Dodd,Catherine Moss,Alison Murphy,Derek P Brazil,Gerard Cagney,Dermot F Brougham,Rabi Murad,Darren Finlay,Kristiina Vuori,John CreanPubMed 36435939
- eLife 11:2022Sex-specific role of myostatin signaling in neonatal muscle growth, denervation atrophy, and neuromuscular contractures.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarianne E Emmert,Parul Aggarwal,Kritton Shay-Winkler,Se-Jin Lee,Qingnian Goh,Roger CornwallPubMed 36314781
- Scientific reports 12:139402022Verteporfin ameliorates fibrotic aspects of Dupuytren's disease nodular fibroblasts irrespective the activation state of the cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNataly Puerta Cavanzo,Sophie A Riesmeijer,Iris L Holt-Kedde,Paul M N Werker,Bram Piersma,Peter Olinga,Ruud A BankPubMed 35977978
- BMC genomics 23:5252022Deep learning for de-convolution of Smad2 versus Smad3 binding sites.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJeremy W K Ng,Esther H Q Ong,Lisa Tucker-Kellogg,Greg Tucker-KelloggPubMed 35858839
- iScience 25:1044592022MASTL is enriched in cancerous and pluripotent stem cells and influences OCT1/OCT4 levels.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElisa Närvä,Maria E Taskinen,Sergio Lilla,Aleksi Isomursu,Mika Pietilä,Jere Weltner,Jorma Isola,Harri Sihto,Heikki Joensuu,Sara Zanivan,Jim Norman,Johanna IvaskaPubMed 35677646
- Biology 11:2022D-Carvone Attenuates CCl-Induced Liver Fibrosis in Rats by Inhibiting Oxidative Stress and TGF-ß 1/SMAD3 Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHanan A Ogaly,Sharah A A Aldulmani,Fatimah A M Al-Zahrani,Reham M Abd-ElsalamPubMed 35625467
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
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Target data
Function
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Alternative names
MADH3, SMAD3, Mothers against decapentaplegic homolog 3, MAD homolog 3, Mad3, Mothers against DPP homolog 3, hMAD-3, JV15-2, SMAD family member 3, SMAD 3, Smad3, hSMAD3
Recommended products
Rabbit Polyclonal SMAD3 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 186 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
This antibody is affinity purified.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Smad3 also known as Mothers against decapentaplegic homolog 3 is a protein that plays a mechanical role in signal transduction. It acts mainly as a transcription factor and gets activated through phosphorylation. The molecular weight of Smad3 is approximately 48 kDa. It is expressed widely across numerous tissues including the cellular nucleus where it executes its function after activation.
- Biological function summary
Smad3 acts as a mediator of signal transduction for the TGF-beta (transforming growth factor-beta) superfamily forming a complex with phosphorylated Smad2. This enables it to regulate transcriptional activity influencing cell proliferation differentiation and apoptosis. Smad3 also participates in various cellular processes by interacting with other co-factors and regulatory proteins that aid in fine-tuning its function.
- Pathways
Smad3 plays an important role in the TGF-beta signaling pathway where it works closely with Smad4 to propagate the signal. Upon phosphorylation it forms a complex with co-Smad (Smad4) and moves into the nucleus to influence gene expression. Smad3 is also involved in pathways related to oncogenesis and tissue fibrosis indicating its significant role in cellular regulation and response mechanisms.
- Associated diseases and disorders
Smad3 is associated with fibrotic diseases and cancers particularly in tissues such as the liver and lungs. Altered Smad3 signaling contributes to the pathological process occurring in fibrotic disorders often interacting with Smad4 in these abnormalities. Dysregulated Smad3 expression or mutations can also lead to oncogenic transformations highlighting its critical involvement in disease states.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad3 antibody (ab28379)
ab28379, at a 1/100 dilution, staining Smad3 in formalin fixed paraffin embedded human breast carcinoma sections by
Immunohistochemistry.
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