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AB227223

Anti-Smad3 antibody - ChIP Grade

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(7 Publications)

Rabbit Polyclonal SMAD3 antibody. Suitable for IP, ChIP, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SMAD3.

View Alternative Names

MADH3, SMAD3, Mothers against decapentaplegic homolog 3, MAD homolog 3, Mad3, Mothers against DPP homolog 3, hMAD-3, JV15-2, SMAD family member 3, SMAD 3, Smad3, hSMAD3

7 Images
Immunocytochemistry/ Immunofluorescence - Anti-Smad3 antibody - ChIP Grade (AB227223)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Smad3 antibody - ChIP Grade (AB227223)

PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Smad3 (green) using ab227223 at 1/200 dilution in ICC/IF.

Nuclear Counterstain : Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad3 antibody - ChIP Grade (AB227223)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad3 antibody - ChIP Grade (AB227223)

Paraffin-embedded human gastric cancer tissue stained for Smad3 using ab227223 at 1/500 dilution in immunohistochemical analysis.

ChIP - Anti-Smad3 antibody - ChIP Grade (AB227223)
  • ChIP

Supplier Data

ChIP - Anti-Smad3 antibody - ChIP Grade (AB227223)

Chromatin was prepared from Hela (human epithelial cell line from cervix adenocarcinoma) whole cell lysate. The ChIP was performed with 5 μg of preimmune rabbit IgG (Lane 1) and 5 μg of ab227223 (Lane 2). The immunoprecipitated DNA was detected by PCR with a primer set targeting to the smad7 promoter.

Immunoprecipitation - Anti-Smad3 antibody - ChIP Grade (AB227223)
  • IP

Supplier Data

Immunoprecipitation - Anti-Smad3 antibody - ChIP Grade (AB227223)

Smad3 was immunoprecipitated from HeLa (human epitheilal cell line from cervix adenocarcinoma) whole cell lysate with 2 μg of ab227223. Western blot was performed from the immunoprecipitate using ab227223 at 1/1000 dilution. Anti-rabbit IgG was used as secondary antibody.

Lane 1 : HeLa whole cell lysate 50 μg (Input).

Lane 2 : Preimmune rabbit IgG instead of ab227223 in HeLa whole cell lysate.

Lane 3 : ab227223 IP in HeLa whole cell lysate.

All lanes:

Immunoprecipitation - Anti-Smad3 antibody - ChIP Grade (ab227223)

Predicted band size: 48 kDa

false

Immunoprecipitation - Anti-Smad3 antibody - ChIP Grade (AB227223)
  • IP

Supplier Data

Immunoprecipitation - Anti-Smad3 antibody - ChIP Grade (AB227223)

Immunoprecipitation of SMAD2 / SMAD3 protein from HeLa whole cell extracts using 2 µg of SMAD2 / SMAD3 antibody.
Western blot analysis was performed using SMAD2 / SMAD3 antibody at a 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-Smad3 antibody - ChIP Grade (ab227223) at 1/1000 dilution

Lane 1:

HeLa whole cell extracts (Input)

Lane 2:

HeLa whole cell extracts with control IgG (-)

Lane 3:

HeLa whole cell extracts (+)

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Observed band size: 60 kDa

false

Western blot - Anti-Smad3 antibody - ChIP Grade (AB227223)
  • WB

Supplier Data

Western blot - Anti-Smad3 antibody - ChIP Grade (AB227223)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Smad3 antibody - ChIP Grade (ab227223) at 1/20000 dilution

Lane 1:

Non-transfected 293T whole cell extract at 30 µg

Lane 2:

SMAD1 transfected 293T whole cell extracts at 30 µg

Lane 3:

SMAD2 transfected 293T whole cell extracts at 30 µg

Lane 4:

SMAD3 transfected 293T whole cell extracts at 30 µg

Lane 5:

SMAD4 transfected 293T whole cell extracts at 30 µg

Lane 6:

SMAD5 transfected 293T whole cell extracts at 30 µg

Lane 7:

SMAD9 transfected 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 60 kDa

false

Western blot - Anti-Smad3 antibody - ChIP Grade (AB227223)
  • WB

Supplier Data

Western blot - Anti-Smad3 antibody - ChIP Grade (AB227223)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Smad3 antibody - ChIP Grade (ab227223) at 1/5000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Lane 3:

HeLa whole cell extracts at 30 µg

Lane 4:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 60 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, ChIP, ICC/IF, WB, IP

applications

Immunogen

Recombinant Fragment Protein within Human SMAD3. The exact immunogen used to generate this antibody is proprietary information.

P84022

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/1000", "IP-species-notes": "<p></p>", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Smad3 also known as Mothers against decapentaplegic homolog 3 is a protein that plays a mechanical role in signal transduction. It acts mainly as a transcription factor and gets activated through phosphorylation. The molecular weight of Smad3 is approximately 48 kDa. It is expressed widely across numerous tissues including the cellular nucleus where it executes its function after activation.
Biological function summary

Smad3 acts as a mediator of signal transduction for the TGF-beta (transforming growth factor-beta) superfamily forming a complex with phosphorylated Smad2. This enables it to regulate transcriptional activity influencing cell proliferation differentiation and apoptosis. Smad3 also participates in various cellular processes by interacting with other co-factors and regulatory proteins that aid in fine-tuning its function.

Pathways

Smad3 plays an important role in the TGF-beta signaling pathway where it works closely with Smad4 to propagate the signal. Upon phosphorylation it forms a complex with co-Smad (Smad4) and moves into the nucleus to influence gene expression. Smad3 is also involved in pathways related to oncogenesis and tissue fibrosis indicating its significant role in cellular regulation and response mechanisms.

Smad3 is associated with fibrotic diseases and cancers particularly in tissues such as the liver and lungs. Altered Smad3 signaling contributes to the pathological process occurring in fibrotic disorders often interacting with Smad4 in these abnormalities. Dysregulated Smad3 expression or mutations can also lead to oncogenic transformations highlighting its critical involvement in disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

SMAD3 is a receptor-regulated SMAD that functions as an intracellular signal transducer and transcriptional modulator, activated by TGF-beta and activin type 1 receptor kinases. It binds to the TRE element in promoters of numerous genes regulated by TGF-beta, and upon forming a complex with SMAD4, activates transcription. Additionally, SMAD3 can form a complex with SMAD4, JUN, and FOS at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. SMAD3 may inhibit wound healing by modulating the growth and migration of primary keratinocytes and altering TGF-beta-mediated monocyte chemotaxis, with this effect being potentially hormone-sensitive. Furthermore, SMAD3 is involved in regulating chondrogenesis and osteogenesis and may inhibit early bone fracture healing. It also positively regulates PDPK1 kinase activity by promoting its dissociation from the 14-3-3 protein YWHAQ, which negatively regulates it. This supplementary information is collated from multiple sources and compiled automatically.
See full target information SMAD3
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

BMC nephrology 25:64 PubMed38395806

2024

Assessing the effects of tempol on renal fibrosis, inflammation, and oxidative stress in a high-salt diet combined with 5/6 nephrectomy rat model: utilizing oxidized albumin as a biomarker.

Applications

Unspecified application

Species

Unspecified reactive species

Beibei Liu,Yanling Hu,Danyang Tian,Jianlong Dong,Bing-Feng Li

Nature genetics 56:85-99 PubMed38092881

2023

A chromatin-regulated biphasic circuit coordinates IL-1β-mediated inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Ezio T Fok,Simone J C F M Moorlag,Yutaka Negishi,Laszlo A Groh,Jéssica Cristina Dos Santos,Cathrin Gräwe,Valerie Villacorta Monge,Daphne D D Craenmehr,Mellanie van Roosmalen,David Pablo da Cunha Jolvino,Letícia Busato Migliorini,Ary Serpa Neto,Patricia Severino,Michiel Vermeulen,Leo A B Joosten,Mihai G Netea,Stephanie Fanucchi,Musa M Mhlanga

Oncogene 43:47-60 PubMed37935976

2023

Low expression of ZFP36L1 in osteosarcoma promotes lung metastasis by inhibiting the SDC4-TGF-β signaling feedback loop.

Applications

Unspecified application

Species

Unspecified reactive species

Mengjun Ma,Jiahao Zhuang,Hongyu Li,Rujia Mi,Yihui Song,Wen Yang,Yixuan Lu,Xin Shen,Yanfeng Wu,Huiyong Shen

BMC cardiovascular disorders 22:17 PubMed35081907

2022

Resveratrol ameliorates myocardial fibrosis by regulating Sirt1/Smad3 deacetylation pathway in rat model with dilated cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Qingquan Chen,Yu Zeng,Xiulin Yang,Yue Wu,Shuyu Zhang,Shirong Huang,Yameng Zhong,Min Chen

Frontiers in pharmacology 12:646240 PubMed34177571

2021

Resveratrol Ameliorates Cardiac Remodeling in a Murine Model of Heart Failure With Preserved Ejection Fraction.

Applications

Unspecified application

Species

Unspecified reactive species

Liyun Zhang,Juan Chen,Lianhua Yan,Qin He,Han Xie,Manhua Chen

Molecular therapy. Nucleic acids 22:673-683 PubMed33230465

2020

Overexpression of circRNA circUCK2 Attenuates Cell Apoptosis in Cerebral Ischemia-Reperfusion Injury via miR-125b-5p/GDF11 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Wanghao Chen,Hong Wang,Jia Feng,Lukui Chen

BMC veterinary research 15:48 PubMed30709353

2019

All-trans retinoic acid inhibits lipopolysaccharide-induced inflammatory responses in bovine adipocytes via TGFβ1/Smad3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qiushi Xu,Hongdou Jia,Li Ma,Guowen Liu,Chuang Xu,Ying Li,Xinwei Li,Xiaobing Li
View all publications
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