AB63402

Anti-Smad3 (phospho S204) antibody

Name: Anti-Smad3 (phospho S204) antibody (ab63402) | Abcam
Brand: Abcam Limited
SKU: AB63402
Price: 545 USD
Availability: InStock

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(9 Publications)

Rabbit Polyclonal SMAD3 phospho S204 antibody. Suitable for WB and reacts with Mouse samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human SMAD3 phospho S204.

View Alternative Names

MADH3, SMAD3, Mothers against decapentaplegic homolog 3, MAD homolog 3, Mad3, Mothers against DPP homolog 3, hMAD-3, JV15-2, SMAD family member 3, SMAD 3, Smad3, hSMAD3

1 Images

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Western blot - Anti-Smad3 (phospho S204) antibody (AB63402)

The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide is 5-10 ug.

All lanes:

Western blot - Anti-Smad3 (phospho S204) antibody (ab63402) at 1/500 dilution

Lane 1:

Extracts from NIH/3T3 cells, treated with Serum (20%, 15mins)

Lane 2:

Extracts from NIH/3T3 cells, treated with Serum (20%, 15mins) plus phospho peptide

Predicted band size: 48 kDa

Observed band size: 48 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

Synthetic Peptide within Human SMAD3 phospho S204. The exact immunogen used to generate this antibody is proprietary information.

P84022

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Smad3 also known as Mothers against decapentaplegic homolog 3 is a protein that plays a mechanical role in signal transduction. It acts mainly as a transcription factor and gets activated through phosphorylation. The molecular weight of Smad3 is approximately 48 kDa. It is expressed widely across numerous tissues including the cellular nucleus where it executes its function after activation.

Biological function summary

Smad3 acts as a mediator of signal transduction for the TGF-beta (transforming growth factor-beta) superfamily forming a complex with phosphorylated Smad2. This enables it to regulate transcriptional activity influencing cell proliferation differentiation and apoptosis. Smad3 also participates in various cellular processes by interacting with other co-factors and regulatory proteins that aid in fine-tuning its function.

Pathways

Smad3 plays an important role in the TGF-beta signaling pathway where it works closely with Smad4 to propagate the signal. Upon phosphorylation it forms a complex with co-Smad (Smad4) and moves into the nucleus to influence gene expression. Smad3 is also involved in pathways related to oncogenesis and tissue fibrosis indicating its significant role in cellular regulation and response mechanisms.

Smad3 is associated with fibrotic diseases and cancers particularly in tissues such as the liver and lungs. Altered Smad3 signaling contributes to the pathological process occurring in fibrotic disorders often interacting with Smad4 in these abnormalities. Dysregulated Smad3 expression or mutations can also lead to oncogenic transformations highlighting its critical involvement in disease states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

SMAD3 is a receptor-regulated SMAD that functions as an intracellular signal transducer and transcriptional modulator, activated by TGF-beta and activin type 1 receptor kinases. It binds to the TRE element in promoters of numerous genes regulated by TGF-beta, and upon forming a complex with SMAD4, activates transcription. Additionally, SMAD3 can form a complex with SMAD4, JUN, and FOS at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. SMAD3 may inhibit wound healing by modulating the growth and migration of primary keratinocytes and altering TGF-beta-mediated monocyte chemotaxis, with this effect being potentially hormone-sensitive. Furthermore, SMAD3 is involved in regulating chondrogenesis and osteogenesis and may inhibit early bone fracture healing. It also positively regulates PDPK1 kinase activity by promoting its dissociation from the 14-3-3 protein YWHAQ, which negatively regulates it. This supplementary information is collated from multiple sources and compiled automatically.

See full target information SMAD3 phospho S204

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Allergologia et immunopathologia 52:91-96 PubMed38970271

2024

Jolkinolide B attenuates allergic airway inflammation and airway remodeling in asthmatic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Lin,Chao Xu,Jintong Ge,Hua Wu,Qi Wang

Biomolecules & biomedicine : PubMed38059910

2023

Mechanism of adipose tissue-derived stromal cell-extracellular vesicles in treating oral submucous fibrosis by blocking the TGF-β1/Smad3 pathway via the miR-760-3p/IGF1R axis

Applications

Unspecified application

Species

Unspecified reactive species

Fengcong Wang,Li Jiang,Ping Liu,Yanjun Jiang

Pharmacology 108:432-443 PubMed37343534

2023

Quercetin Alleviates Asthma-Induced Airway Inflammation and Remodeling through Downregulating Periostin via Blocking TGF-β1/Smad Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yanni Fang,Wenwen Jin,Zhen Guo,Jumei Hao

Journal of cell science 134: PubMed34338295

2021

A dual role of YAP in driving TGFβ-mediated endothelial-to-mesenchymal transition.

Applications

Unspecified application

Species

Unspecified reactive species

Cecilia Savorani,Matteo Malinverno,Roberta Seccia,Claudio Maderna,Monica Giannotta,Linda Terreran,Eleonora Mastrapasqua,Stefano Campaner,Elisabetta Dejana,Costanza Giampietro

Cancer biology & therapy 21:994-1004 PubMed33054513

2020

Cyclin E overexpression confers resistance to trastuzumab through noncanonical phosphorylation of SMAD3 in HER2+ breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph T Decker,Pridvi Kandagatla,Lei Wan,Regan Bernstein,Jeffrey A Ma,Lonnie D Shea,Jacqueline S Jeruss

Experimental lung research 45:245-254 PubMed31496319

2019

Atrial natriuretic peptide inhibits epithelial-mesenchymal transition (EMT) of bronchial epithelial cells through cGMP/PKG signaling by targeting Smad3 in a murine model of allergic asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Shuyuan Chu,Xiufeng Zhang,Yabing Sun,Yaxi Liang,Jingyi Sun,Minyan Lu,Jianwei Huang,Ming Jiang,Libing Ma

Oncology reports 41:224-234 PubMed30542739

2018

sFRP1 exerts effects on gastric cancer cells through GSK3β/Rac1‑mediated restraint of TGFβ/Smad3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Xiang Peng,Shun-Yu Liang,Li Li

Cancer biology & therapy 19:271-281 PubMed29053396

2018

Embryonic stem cell secreted factors decrease invasiveness of triple-negative breast cancer cells through regulome modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth Tarasewicz,Robert S Oakes,Misael O Aviles,Joelle Straehla,Kathryn M Chilton,Joseph T Decker,Jia Wu,Lonnie D Shea,Jacqueline S Jeruss

Experimental cell research 319:2928-37 PubMed24080014

2013

Serine-204 in the linker region of Smad3 mediates the collagen-I response to TGF-β in a cell phenotype-specific manner.

Applications

Unspecified application

Species

Unspecified reactive species

J A Browne,X Liu,H W Schnaper,T Hayashida

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Smad3 (phospho S204) antibody

Western blot - Anti-Smad3 (phospho S204) antibody (AB63402)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Complementary Products

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Primary Antibodies

Primary Antibodies

Secondary Antibodies

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