AB138659

Anti-Smad3 (phospho S208) antibody

Name: Anti-Smad3 (phospho S208) antibody (ab138659) | Abcam
Brand: Abcam Limited
SKU: AB138659
Price: 535 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal SMAD3 phospho S208 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human SMAD3 phospho S208.

View Alternative Names

MADH3, SMAD3, Mothers against decapentaplegic homolog 3, MAD homolog 3, Mad3, Mothers against DPP homolog 3, hMAD-3, JV15-2, SMAD family member 3, SMAD 3, Smad3, hSMAD3

1 Images

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Western blot - Anti-Smad3 (phospho S208) antibody (AB138659)

All lanes:

Western blot - Anti-Smad3 (phospho S208) antibody (ab138659) at 1/500 dilution

All lanes:

Jurkat cell lysate at 30 µg

Predicted band size: 48 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human SMAD3 phospho S208. The exact immunogen used to generate this antibody is proprietary information.

P84022

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab138659 was purified from Rabbit antiserum. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Smad3 also known as Mothers against decapentaplegic homolog 3 is a protein that plays a mechanical role in signal transduction. It acts mainly as a transcription factor and gets activated through phosphorylation. The molecular weight of Smad3 is approximately 48 kDa. It is expressed widely across numerous tissues including the cellular nucleus where it executes its function after activation.

Biological function summary

Smad3 acts as a mediator of signal transduction for the TGF-beta (transforming growth factor-beta) superfamily forming a complex with phosphorylated Smad2. This enables it to regulate transcriptional activity influencing cell proliferation differentiation and apoptosis. Smad3 also participates in various cellular processes by interacting with other co-factors and regulatory proteins that aid in fine-tuning its function.

Pathways

Smad3 plays an important role in the TGF-beta signaling pathway where it works closely with Smad4 to propagate the signal. Upon phosphorylation it forms a complex with co-Smad (Smad4) and moves into the nucleus to influence gene expression. Smad3 is also involved in pathways related to oncogenesis and tissue fibrosis indicating its significant role in cellular regulation and response mechanisms.

Smad3 is associated with fibrotic diseases and cancers particularly in tissues such as the liver and lungs. Altered Smad3 signaling contributes to the pathological process occurring in fibrotic disorders often interacting with Smad4 in these abnormalities. Dysregulated Smad3 expression or mutations can also lead to oncogenic transformations highlighting its critical involvement in disease states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

SMAD3 is a receptor-regulated SMAD that functions as an intracellular signal transducer and transcriptional modulator, activated by TGF-beta and activin type 1 receptor kinases. It binds to the TRE element in promoters of numerous genes regulated by TGF-beta, and upon forming a complex with SMAD4, activates transcription. Additionally, SMAD3 can form a complex with SMAD4, JUN, and FOS at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. SMAD3 may inhibit wound healing by modulating the growth and migration of primary keratinocytes and altering TGF-beta-mediated monocyte chemotaxis, with this effect being potentially hormone-sensitive. Furthermore, SMAD3 is involved in regulating chondrogenesis and osteogenesis and may inhibit early bone fracture healing. It also positively regulates PDPK1 kinase activity by promoting its dissociation from the 14-3-3 protein YWHAQ, which negatively regulates it. This supplementary information is collated from multiple sources and compiled automatically.

See full target information SMAD3 phospho S208

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Chemico-biological interactions 319:109024 PubMed32097614

2020

Tanshinone IIA attenuates silica-induced pulmonary fibrosis via Nrf2-mediated inhibition of EMT and TGF-β1/Smad signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Feifei Feng,Peng Cheng,Shaohua Xu,Nannan Li,Hui Wang,Ying Zhang,Wei Wang

Cell death & disease 11:136 PubMed32075954

2020

Microcystin-LR ameliorates pulmonary fibrosis via modulating CD206 M2-like macrophage polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Wang,Lizhi Xu,Zou Xiang,Yan Ren,Xiufen Zheng,Qingya Zhao,Qunzhi Zhou,Yuefen Zhou,Lin Xu,Yaping Wang

Drug design, development and therapy 13:4275-4290 PubMed31908414

2019

The Protective Role of Tanshinone IIA in Silicosis Rat Model via TGF-β1/Smad Signaling Suppression, NOX4 Inhibition and Nrf2/ARE Signaling Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Feifei Feng,Peng Cheng,Huanan Zhang,Nannan Li,Yuxin Qi,Hui Wang,Yongbin Wang,Wei Wang

Oncotarget 10:1716-1728 PubMed30899443

2019

Statins affect human glioblastoma and other cancers through TGF-β inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Aizhen Xiao,Breanna Brenneman,Desiree Floyd,Laurey Comeau,Kelsey Spurio,Inan Olmez,Jeongwu Lee,Ichiro Nakano,Jakub Godlewski,Agnieszka Bronisz,Noritaka Kagaya,Kazuo Shin-Ya,Benjamin Purow

Molecular medicine reports 14:1610-6 PubMed27315199

2016

Jia-Shen decoction-medicated serum inhibits angiotensin-II induced cardiac fibroblast proliferation via the TGF-β1/Smad signaling pathway.

Applications

Species

Rat

Lin Cui,Youping Wang,Rui Yu,Bin Li,Shiyang Xie,Yuan Gao,Xiaoxiao Wang,Mingjun Zhu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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