AB63403

Anti-Smad3 (phospho S213) antibody

Name: Anti-Smad3 (phospho S213) antibody (ab63403) | Abcam
Brand: Abcam Limited
SKU: AB63403
Price: 525 USD
Availability: InStock
Rating: 5 (3 reviews)

(3 Reviews)

(37 Publications)

Rabbit Polyclonal SMAD3 phospho S213 antibody. Suitable for ICC, ELISA, WB, ICC/IF and reacts with Human samples. Cited in 37 publications. Immunogen corresponding to Synthetic Peptide within Human SMAD3 phospho S213.

View Alternative Names

MADH3, SMAD3, Mothers against decapentaplegic homolog 3, MAD homolog 3, Mad3, Mothers against DPP homolog 3, hMAD-3, JV15-2, SMAD family member 3, SMAD 3, Smad3, hSMAD3

1 Images

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Western blot - Anti-Smad3 (phospho S213) antibody (AB63403)

The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide is 5-10 ug.

All lanes:

Western blot - Anti-Smad3 (phospho S213) antibody (ab63403) at 1/500 dilution

Lane 1:

Extracts from HT29 cells

Lane 2:

Extracts from HT29 cells plus phospho peptide

Predicted band size: 48 kDa

Observed band size: 48 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, ICC, ELISA

applications

Immunogen

Synthetic Peptide within Human SMAD3 phospho S213. The exact immunogen used to generate this antibody is proprietary information.

P84022

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Smad3 also known as Mothers against decapentaplegic homolog 3 is a protein that plays a mechanical role in signal transduction. It acts mainly as a transcription factor and gets activated through phosphorylation. The molecular weight of Smad3 is approximately 48 kDa. It is expressed widely across numerous tissues including the cellular nucleus where it executes its function after activation.

Biological function summary

Smad3 acts as a mediator of signal transduction for the TGF-beta (transforming growth factor-beta) superfamily forming a complex with phosphorylated Smad2. This enables it to regulate transcriptional activity influencing cell proliferation differentiation and apoptosis. Smad3 also participates in various cellular processes by interacting with other co-factors and regulatory proteins that aid in fine-tuning its function.

Pathways

Smad3 plays an important role in the TGF-beta signaling pathway where it works closely with Smad4 to propagate the signal. Upon phosphorylation it forms a complex with co-Smad (Smad4) and moves into the nucleus to influence gene expression. Smad3 is also involved in pathways related to oncogenesis and tissue fibrosis indicating its significant role in cellular regulation and response mechanisms.

Smad3 is associated with fibrotic diseases and cancers particularly in tissues such as the liver and lungs. Altered Smad3 signaling contributes to the pathological process occurring in fibrotic disorders often interacting with Smad4 in these abnormalities. Dysregulated Smad3 expression or mutations can also lead to oncogenic transformations highlighting its critical involvement in disease states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

SMAD3 is a receptor-regulated SMAD that functions as an intracellular signal transducer and transcriptional modulator, activated by TGF-beta and activin type 1 receptor kinases. It binds to the TRE element in promoters of numerous genes regulated by TGF-beta, and upon forming a complex with SMAD4, activates transcription. Additionally, SMAD3 can form a complex with SMAD4, JUN, and FOS at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. SMAD3 may inhibit wound healing by modulating the growth and migration of primary keratinocytes and altering TGF-beta-mediated monocyte chemotaxis, with this effect being potentially hormone-sensitive. Furthermore, SMAD3 is involved in regulating chondrogenesis and osteogenesis and may inhibit early bone fracture healing. It also positively regulates PDPK1 kinase activity by promoting its dissociation from the 14-3-3 protein YWHAQ, which negatively regulates it. This supplementary information is collated from multiple sources and compiled automatically.

See full target information SMAD3 phospho S213

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Publications (37)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 23:230 PubMed40382656

2025

Prkci promotes colorectal cancer metastasis by phosphorylating and stabilizing Tgfbr1 to activate TGF-β signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Li,Guangshi Liu,Wenbin Zhang,Tao Li

Circulation 150:132-150 PubMed38557054

2024

BRCC3 Regulation of ALK2 in Vascular Smooth Muscle Cells: Implication in Pulmonary Hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Shen,Ya Gao,Dedong Ge,Meng Tan,Qing Yin,Tong-You Wade Wei,Fangzhou He,Tzong-Yi Lee,Zhongyan Li,Yuqin Chen,Qifeng Yang,Zhangyu Liu,Xinxin Li,Zixuan Chen,Yi Yang,Zhengang Zhang,Patricia A Thistlethwaite,Jian Wang,Atul Malhotra,Jason X-J Yuan,John Y-J Shyy,Kaizheng Gong

Cellular and molecular gastroenterology and hepatology 17:567-587 PubMed38154598

2023

The Interplay of TGF-β1 and Cholesterol Orchestrating Hepatocyte Cell Fate, EMT, and Signals for HSC Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Sai Wang,Frederik Link,Mei Han,Roohi Chaudhary,Anastasia Asimakopoulos,Roman Liebe,Ye Yao,Seddik Hammad,Anne Dropmann,Marinela Krizanac,Claudia Rubie,Laura Kim Feiner,Matthias Glanemann,Matthias P A Ebert,Ralf Weiskirchen,Yoav I Henis,Marcelo Ehrlich,Steven Dooley

Cell death & disease 14:822 PubMed38092725

2023

Jagged1 intracellular domain/SMAD3 complex transcriptionally regulates TWIST1 to drive glioma invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Jung Yun Kim,Nayoung Hong,Sehyeon Park,Seok Won Ham,Eun-Jung Kim,Sung-Ok Kim,Junseok Jang,Yoonji Kim,Jun-Kyum Kim,Sung-Chan Kim,Jong-Whi Park,Hyunggee Kim

Molecular medicine (Cambridge, Mass.) 29:155 PubMed37940881

2023

Exosomal circCOL1A1 promotes angiogenesis via recruiting EIF4A3 protein and activating Smad2/3 pathway in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Gui Hu,Changwei Lin,Kai Gao,Miao Chen,Fei Long,Buning Tian

Experimental and therapeutic medicine 26:572 PubMed38023356

2023

Dapagliflozin can alleviate renal fibrosis in rats with streptozotocin‑induced type 2 diabetes mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

Song Xue,Ying-Xuan Li,Xiao-Xiao Lu,Wei Tang

Phytotherapy research : PTR 38:1745-1760 PubMed37740455

2023

Ginsenoside RG1-induced mesenchymal stem cells alleviate diabetic cardiomyopathy through secreting exosomal circNOTCH1 to promote macrophage M2 polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Zhen,Jinping Bai,Jia Liu,Hongbo Men,Haitao Yu

Cellular and molecular life sciences : CMLS 80:156 PubMed37208565

2023

CSNK1D-mediated phosphorylation of HNRNPA2B1 induces miR-25-3p/miR-93-5p maturation to promote prostate cancer cell proliferation and migration through mA-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Qi,Wenyi Shen,Xiyi Wei,Yifei Cheng,Fan Xu,Yuxiao Zheng,Lu Li,Chao Qin,Xiao Li

Renal failure 45:2120821 PubMed36648018

2023

Combination therapy with DHA and BMSCs suppressed podocyte injury and attenuated renal fibrosis by modulating the TGF-1/Smad pathway in MN mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yongzhang Li,Suzhi Chen,Jinchuan Tan,Yan Zhou,Meifang Ren,Qian Zhang,Meijiao Zhao,Guodong Yuan,Wenxi Zhang,Fengwen Yang

Nature communications 13:6069 PubMed36241625

2022

Opposing functions of circadian protein DBP and atypical E2F family E2F8 in anti-tumor Th9 cell differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Sang-A Park,Yun-Ji Lim,Wai Lim Ku,Dunfang Zhang,Kairong Cui,Liu-Ya Tang,Cheryl Chia,Peter Zanvit,Zuojia Chen,Wenwen Jin,Dandan Wang,Junji Xu,Ousheng Liu,Fu Wang,Alexander Cain,Nancy Guo,Hiroko Nakatsukasa,Chuan Wu,Ying E Zhang,Keji Zhao,WanJun Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Smad3 (phospho S213) antibody

Western blot - Anti-Smad3 (phospho S213) antibody (AB63403)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (37)

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