Skip to main content

Rabbit Recombinant Monoclonal SMAD3 phospho S423 + S425 antibody. Suitable for Dot, WB, ICC/IF, IHC-P, ChIC/CUT&RUN-seq and reacts with Human, Mouse samples. Cited in 576 publications.

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytDotWBICC/IFIHC-PChIC/CUT&RUN-seq
Human
Not recommended
Not recommended
Tested
Tested
Tested
Tested
Tested
Mouse
Not recommended
Not recommended
Expected
Tested
Expected
Tested
Expected

Images

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/2000
Notes

-

Species
Human
Dilution info
1/2000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100 - 1/250
Notes

The secondary antibody is rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species
Human
Dilution info
1/100 - 1/250
Notes

The secondary antibody is rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

Rabbit Recombinant Monoclonal SMAD3 phospho S423 + S425 antibody. Suitable for Dot, WB, ICC/IF, IHC-P, ChIC/CUT&RUN-seq and reacts with Human, Mouse samples. Cited in 576 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Clone number

EP823Y

Purification technique

Affinity purification Protein A

Specificity

This antibody detects Smad3 phosphorylated on Serine 423 and Serine 425. This Smad3 antibody may also detect Smad1, Smad2 and Smad5 phosphorylated at the equivalent sites.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

There was a problem

We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com