AB193297

Anti-Smad3 (phospho T179) antibody

Name: Anti-Smad3 (phospho T179) antibody (ab193297) | Abcam
Brand: Abcam Limited
SKU: AB193297
Price: 535 USD
Availability: InStock

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(13 Publications)

Rabbit Polyclonal SMAD3 phospho T179 antibody. Suitable for WB and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human SMAD3 phospho T179 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

MADH3, SMAD3, SMAD family member 3, SMAD 3, hSMAD3, JV15-2, Mothers against decapentaplegic homolog 3, MAD homolog 3, Mad3, Mothers against DPP homolog 3, hMAD-3

1 Images

Supplier Data

Western blot - Anti-Smad3 (phospho T179) antibody (AB193297)

All lanes:

Western blot - Anti-Smad3 (phospho T179) antibody (ab193297) at 1/500 dilution

Lane 1:

extracts from HeLa cells treated with TNF

Lane 2:

extracts from HeLa cells treated with TNF with antigen-specific peptide

Predicted band size: 48 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human SMAD3 phospho T179 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P84022

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab193297 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Smad3 also known as Mothers against decapentaplegic homolog 3 is a protein that plays a mechanical role in signal transduction. It acts mainly as a transcription factor and gets activated through phosphorylation. The molecular weight of Smad3 is approximately 48 kDa. It is expressed widely across numerous tissues including the cellular nucleus where it executes its function after activation.

Biological function summary

Smad3 acts as a mediator of signal transduction for the TGF-beta (transforming growth factor-beta) superfamily forming a complex with phosphorylated Smad2. This enables it to regulate transcriptional activity influencing cell proliferation differentiation and apoptosis. Smad3 also participates in various cellular processes by interacting with other co-factors and regulatory proteins that aid in fine-tuning its function.

Pathways

Smad3 plays an important role in the TGF-beta signaling pathway where it works closely with Smad4 to propagate the signal. Upon phosphorylation it forms a complex with co-Smad (Smad4) and moves into the nucleus to influence gene expression. Smad3 is also involved in pathways related to oncogenesis and tissue fibrosis indicating its significant role in cellular regulation and response mechanisms.

Smad3 is associated with fibrotic diseases and cancers particularly in tissues such as the liver and lungs. Altered Smad3 signaling contributes to the pathological process occurring in fibrotic disorders often interacting with Smad4 in these abnormalities. Dysregulated Smad3 expression or mutations can also lead to oncogenic transformations highlighting its critical involvement in disease states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

See full target information SMAD3 phospho T179

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 22:1457 PubMed34737797

2021

Electroacupuncture regulates inflammation, collagen deposition and macrophage function in skeletal muscle through the TGF-β1/Smad3/p38/ERK1/2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Han,Ming Li,Huilin Liu,Haohan Li

Evidence-based complementary and alternative medicine : eCAM 2021:5649575 PubMed34422075

2021

Antifibrotic Effects of Kangxian Ruangan Capsule on Rats with Nonalcoholic Fatty Liver Fibrosis and Hepatic Stellate Cells through Regulation of TGF- and TLR4 Signaling Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Liming Liu,Ying Zhou,Dan Dai,Hongmei Xia,Kang Zhao,Jianjun Zhang

Frontiers in molecular biosciences 8:697773 PubMed34222341

2021

LINC00174 Facilitates Cell Proliferation, Cell Migration and Tumor Growth of Osteosarcoma Regulating the TGF-β/SMAD Signaling Pathway and Upregulating SSH2 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Changjun Zheng,Ronghang Li,Shuang Zheng,Hongjuan Fang,Meng Xu,Lei Zhong

Journal of orthopaedic translation 29:30-41 PubMed34094856

2021

Losartan protects against osteoarthritis by repressing the TGF-β1 signaling pathway upregulation of PPARγ.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenhan Deng,Fei Chen,Yuwei Liu,Jinping Wang,Wei Lu,Wei Jiang,Weimin Zhu

British journal of pharmacology 178:1037-1054 PubMed33305374

2021

The antagonistic effects and mechanisms of microRNA-26a action in hypertensive vascular remodelling.

Applications

Unspecified application

Species

Unspecified reactive species

Wenqian Zhang,Qiaozhu Wang,Xin Xing,Lijun Yang,Min Xu,Chunhui Cao,Rong Wang,Weicheng Li,Xiaolin Niu,Dengfeng Gao

Brazilian journal of medical and biological resear 54:e9206 PubMed33503202

2021

MicroRNA-302b mitigates renal fibrosis via inhibiting TGF-β/Smad pathway activation.

Applications

Unspecified application

Species

Unspecified reactive species

Mengkui Sun,Wei Zhou,Fei Yao,Jianming Song,Yanan Xu,Zhimei Deng,Hongwang Diao,Shoulin Li

Drug design, development and therapy 14:5431-5439 PubMed33324040

2020

Xanthohumol Inhibits TGF-β1-Induced Cardiac Fibroblasts Activation via Mediating PTEN/Akt/mTOR Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chuanhao Jiang,Ning Xie,Taoli Sun,Wanjun Ma,Bikui Zhang,Wenqun Li

Journal of the American Heart Association 9:e017970 PubMed32865120

2020

MicroRNA-26a Protects the Heart Against Hypertension-Induced Myocardial Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Wenqian Zhang,Qiaozhu Wang,Yanjing Feng,Xuegui Chen,Lijun Yang,Min Xu,Xiaofang Wang,Weicheng Li,Xiaolin Niu,Dengfeng Gao

Acta pharmacologica Sinica 41:1234-1245 PubMed32327724

2020

Nintedanib inhibits keloid fibroblast functions by blocking the phosphorylation of multiple kinases and enhancing receptor internalization.

Applications

Unspecified application

Species

Unspecified reactive species

Bo-Ya Zhou,Wen-Bo Wang,Xiao-Li Wu,Wen-Jie Zhang,Guang-Dong Zhou,Zhen Gao,Wei Liu

Bioscience reports 39: PubMed31729530

2019

Moracin attenuates LPS-induced inflammation in nucleus pulposus cells via Nrf2/HO-1 and NF-κB/TGF-β pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ronghe Gu,Zonggui Huang,Huijiang Liu,Qiwen Qing,Zou Zhuan,Lijing Yang,Zhongyi Su,Weiguo Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Smad3 (phospho T179) antibody

Western blot - Anti-Smad3 (phospho T179) antibody (AB193297)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

Product promise