AB74062

Anti-Smad3 (phospho T179) antibody

Name: Anti-Smad3 (phospho T179) antibody (ab74062) | Abcam
Brand: Abcam Limited
SKU: AB74062
Price: 525 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(13 Publications)

Rabbit Polyclonal SMAD3 phospho T179 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human SMAD3 phospho T179.

View Alternative Names

MADH3, SMAD3, Mothers against decapentaplegic homolog 3, MAD homolog 3, Mad3, Mothers against DPP homolog 3, hMAD-3, JV15-2, SMAD family member 3, SMAD 3, Smad3, hSMAD3

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad3 (phospho T179) antibody (AB74062)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad3 (phospho T179) antibody (AB74062)

ab74062 at 1/50 dilution staining Smad3 in human thymus gland by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.

Unknown

Western blot - Anti-Smad3 (phospho T179) antibody (AB74062)

All lanes:

Western blot - Anti-Smad3 (phospho T179) antibody (ab74062) at 1/500 dilution

Lane 1:

HeLa cell extracts treated with TNF (20ng/ml, 2mins) at 30 µg

Lane 2:

HeLa cell extracts treated with TNF (20ng/ml, 2mins) at 30 µg with immunising peptide

Predicted band size: 48 kDa

Observed band size: 48 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human SMAD3 phospho T179. The exact immunogen used to generate this antibody is proprietary information.

P84022

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab74062 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Smad3 also known as Mothers against decapentaplegic homolog 3 is a protein that plays a mechanical role in signal transduction. It acts mainly as a transcription factor and gets activated through phosphorylation. The molecular weight of Smad3 is approximately 48 kDa. It is expressed widely across numerous tissues including the cellular nucleus where it executes its function after activation.

Biological function summary

Smad3 acts as a mediator of signal transduction for the TGF-beta (transforming growth factor-beta) superfamily forming a complex with phosphorylated Smad2. This enables it to regulate transcriptional activity influencing cell proliferation differentiation and apoptosis. Smad3 also participates in various cellular processes by interacting with other co-factors and regulatory proteins that aid in fine-tuning its function.

Pathways

Smad3 plays an important role in the TGF-beta signaling pathway where it works closely with Smad4 to propagate the signal. Upon phosphorylation it forms a complex with co-Smad (Smad4) and moves into the nucleus to influence gene expression. Smad3 is also involved in pathways related to oncogenesis and tissue fibrosis indicating its significant role in cellular regulation and response mechanisms.

Smad3 is associated with fibrotic diseases and cancers particularly in tissues such as the liver and lungs. Altered Smad3 signaling contributes to the pathological process occurring in fibrotic disorders often interacting with Smad4 in these abnormalities. Dysregulated Smad3 expression or mutations can also lead to oncogenic transformations highlighting its critical involvement in disease states.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

SMAD3 is a receptor-regulated SMAD that functions as an intracellular signal transducer and transcriptional modulator, activated by TGF-beta and activin type 1 receptor kinases. It binds to the TRE element in promoters of numerous genes regulated by TGF-beta, and upon forming a complex with SMAD4, activates transcription. Additionally, SMAD3 can form a complex with SMAD4, JUN, and FOS at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. SMAD3 may inhibit wound healing by modulating the growth and migration of primary keratinocytes and altering TGF-beta-mediated monocyte chemotaxis, with this effect being potentially hormone-sensitive. Furthermore, SMAD3 is involved in regulating chondrogenesis and osteogenesis and may inhibit early bone fracture healing. It also positively regulates PDPK1 kinase activity by promoting its dissociation from the 14-3-3 protein YWHAQ, which negatively regulates it. This supplementary information is collated from multiple sources and compiled automatically.

See full target information SMAD3 phospho T179

style

left-column

Publications (13)

Recent publications for all applications. Explore the full list and refine your search

PeerJ 12:e18730 PubMed39726752

2024

Malignant behaviors and immune response in melanoma: Epstein-Barr virus induced gene 3 as a therapeutic target based on an exploration.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Zhang,Fengrui Cheng,Xingrui Cai,Jingping Wu

Technology in cancer research & treatment 23:15330338241274337 PubMed39166273

2024

Tumor-Associated Macrophage-Derived TGF-β1 Activates GLI2 via the Smad2/3 Signaling Pathway to Affect Cisplatin Resistance in Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoling Lan,Dalong Wei,Lini Fang,Xiangsheng Wu,Biaoliang Wu

Nature communications 13:6069 PubMed36241625

2022

Opposing functions of circadian protein DBP and atypical E2F family E2F8 in anti-tumor Th9 cell differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Sang-A Park,Yun-Ji Lim,Wai Lim Ku,Dunfang Zhang,Kairong Cui,Liu-Ya Tang,Cheryl Chia,Peter Zanvit,Zuojia Chen,Wenwen Jin,Dandan Wang,Junji Xu,Ousheng Liu,Fu Wang,Alexander Cain,Nancy Guo,Hiroko Nakatsukasa,Chuan Wu,Ying E Zhang,Keji Zhao,WanJun Chen

EBioMedicine 85:104274 PubMed36182775

2022

Inhibition of HIPK2 protects stress-induced pathological cardiac remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Qiulian Zhou,Danni Meng,Feng Li,Xiao Zhang,Li Liu,Yujiao Zhu,Shuqin Liu,Minjun Xu,Jiali Deng,Zhiyong Lei,Joost P G Sluijter,Junjie Xiao

Bioengineered 13:5709-5723 PubMed35188450

2022

microRNA-211-5p predicts the progression of postmenopausal osteoporosis and attenuates osteogenesis by targeting dual specific phosphatase 6.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Wang,Xiaoyan Shi,Zhenye Guo,Feng Zhao,Weifu He,Mingming Kang,Zhi Lv

Journal of materials science. Materials in medicine 33:12 PubMed35050422

2022

Adipose-derived mesenchymal stem cell-loaded β-chitin nanofiber hydrogel promote wound healing in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Yunen Liu,Mi Wu,Rufei Zou,Shun Mao,Peifang Cong,Mingxiao Hou,Hongxu Jin,Yan Zhao,Yongli Bao

Nature communications 12:2809 PubMed33990575

2021

Interplay between transforming growth factor-β and Nur77 in dual regulations of inhibitor of differentiation 1 for colonic tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Boning Niu,Jie Liu,Ben Lv,Jiacheng Lin,Xin Li,Chunxiao Wu,Xiaohua Jiang,Zhiping Zeng,Xiao-Kun Zhang,Hu Zhou

Cancer biology & therapy 21:994-1004 PubMed33054513

2020

Cyclin E overexpression confers resistance to trastuzumab through noncanonical phosphorylation of SMAD3 in HER2+ breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph T Decker,Pridvi Kandagatla,Lei Wan,Regan Bernstein,Jeffrey A Ma,Lonnie D Shea,Jacqueline S Jeruss

International journal of molecular medicine 45:1351-1360 PubMed32323728

2020

Aspirin inhibits endometrial fibrosis by suppressing the TGF‑β1‑Smad2/Smad3 pathway in intrauterine adhesions.

Applications

Unspecified application

Species

Unspecified reactive species

Zihui Zhang,Shuang Li,Jie Deng,Shaorong Yang,Ziwu Xiang,Hongyan Guo,Hongli Xi,Ming Sang,Wei Zhang

Cancer biology & therapy 19:271-281 PubMed29053396

2018

Embryonic stem cell secreted factors decrease invasiveness of triple-negative breast cancer cells through regulome modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth Tarasewicz,Robert S Oakes,Misael O Aviles,Joelle Straehla,Kathryn M Chilton,Joseph T Decker,Jia Wu,Lonnie D Shea,Jacqueline S Jeruss

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Smad3 (phospho T179) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad3 (phospho T179) antibody (AB74062)

Western blot - Anti-Smad3 (phospho T179) antibody (AB74062)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise