Rabbit Polyclonal SMAD3 phospho T179 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human SMAD3 phospho T179.
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- Nature communications 13:60692022Opposing functions of circadian protein DBP and atypical E2F family E2F8 in anti-tumor Th9 cell differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSang-A Park,Yun-Ji Lim,Wai Lim Ku,Dunfang Zhang,Kairong Cui,Liu-Ya Tang,Cheryl Chia,Peter Zanvit,Zuojia Chen,Wenwen Jin,Dandan Wang,Junji Xu,Ousheng Liu,Fu Wang,Alexander Cain,Nancy Guo,Hiroko Nakatsukasa,Chuan Wu,Ying E Zhang,Keji Zhao,WanJun ChenPubMed 36241625
- Journal of materials science. Materials in medicine 33:122022Adipose-derived mesenchymal stem cell-loaded β-chitin nanofiber hydrogel promote wound healing in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Liu,Yunen Liu,Mi Wu,Rufei Zou,Shun Mao,Peifang Cong,Mingxiao Hou,Hongxu Jin,Yan Zhao,Yongli BaoPubMed 35050422
- Nature communications 12:28092021Interplay between transforming growth factor-β and Nur77 in dual regulations of inhibitor of differentiation 1 for colonic tumorigenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBoning Niu,Jie Liu,Ben Lv,Jiacheng Lin,Xin Li,Chunxiao Wu,Xiaohua Jiang,Zhiping Zeng,Xiao-Kun Zhang,Hu ZhouPubMed 33990575
- Cancer biology & therapy 21:994-10042020Cyclin E overexpression confers resistance to trastuzumab through noncanonical phosphorylation of SMAD3 in HER2+ breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoseph T Decker,Pridvi Kandagatla,Lei Wan,Regan Bernstein,Jeffrey A Ma,Lonnie D Shea,Jacqueline S JerussPubMed 33054513
- International journal of molecular medicine 45:1351-13602020Aspirin inhibits endometrial fibrosis by suppressing the TGF‑β1‑Smad2/Smad3 pathway in intrauterine adhesions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZihui Zhang et. al.PubMed 32323728
- Cancer biology & therapy 19:271-2812018Embryonic stem cell secreted factors decrease invasiveness of triple-negative breast cancer cells through regulome modulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElizabeth Tarasewicz et. al.PubMed 29053396
- Oncotarget 8:83925-839392017Synergistic effect of eribulin and CDK inhibition for the treatment of triple negative breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShreyas S Rao et. al.PubMed 29137393
- Oncotarget 6:41650-662015The proinflammatory LTB4/BLT1 signal axis confers resistance to TGF-β1-induced growth inhibition by targeting Smad3 linker region.Applications:IHCReactive species:Unspecified reactive speciesWoo-Kwang Jeon,Jiyeon Choi,Seong Ji Park,Eun Ji Jo,Young K Lee,Seunghwan Lim,Jae-Hong Kim,John J Letterio,Fang Liu,Seong-Jin Kim,Byung-Chul KimPubMed 26497676
- Experimental cell research 319:2928-372013Serine-204 in the linker region of Smad3 mediates the collagen-I response to TGF-β in a cell phenotype-specific manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJ A Browne et. al.PubMed 24080014
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SMAD3 phospho T179. The exact immunogen used to generate this antibody is proprietary information. Database link P84022
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
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Target data
Function
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Alternative names
MADH3, SMAD3, Mothers against decapentaplegic homolog 3, MAD homolog 3, Mad3, Mothers against DPP homolog 3, hMAD-3, JV15-2, SMAD family member 3, SMAD 3, Smad3, hSMAD3
Recommended products
Rabbit Polyclonal SMAD3 phospho T179 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human SMAD3 phospho T179.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SMAD3 phospho T179. The exact immunogen used to generate this antibody is proprietary information. Database link P84022
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab74062 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Smad3 also known as Mothers against decapentaplegic homolog 3 is a protein that plays a mechanical role in signal transduction. It acts mainly as a transcription factor and gets activated through phosphorylation. The molecular weight of Smad3 is approximately 48 kDa. It is expressed widely across numerous tissues including the cellular nucleus where it executes its function after activation.
- Biological function summary
Smad3 acts as a mediator of signal transduction for the TGF-beta (transforming growth factor-beta) superfamily forming a complex with phosphorylated Smad2. This enables it to regulate transcriptional activity influencing cell proliferation differentiation and apoptosis. Smad3 also participates in various cellular processes by interacting with other co-factors and regulatory proteins that aid in fine-tuning its function.
- Pathways
Smad3 plays an important role in the TGF-beta signaling pathway where it works closely with Smad4 to propagate the signal. Upon phosphorylation it forms a complex with co-Smad (Smad4) and moves into the nucleus to influence gene expression. Smad3 is also involved in pathways related to oncogenesis and tissue fibrosis indicating its significant role in cellular regulation and response mechanisms.
- Associated diseases and disorders
Smad3 is associated with fibrotic diseases and cancers particularly in tissues such as the liver and lungs. Altered Smad3 signaling contributes to the pathological process occurring in fibrotic disorders often interacting with Smad4 in these abnormalities. Dysregulated Smad3 expression or mutations can also lead to oncogenic transformations highlighting its critical involvement in disease states.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Smad3 (phospho T179) antibody (ab74062)
All lanes: Western blot - Anti-Smad3 (phospho T179) antibody (ab74062) at 1/500 dilution
Lane 1: HeLa cell extracts treated with TNF (20ng/ml, 2mins) at 30 µg
Lane 2: HeLa cell extracts treated with TNF (20ng/ml, 2mins) at 30 µg with immunising peptide
Predicted band size: 48 kDa
Observed band size: 48 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad3 (phospho T179) antibody (ab74062)
ab74062 at 1/50 dilution staining Smad3 in human thymus gland by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
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