AB236321

Anti-Smad4 antibody

Name: Anti-Smad4 antibody (ab236321) | Abcam
Brand: Abcam Limited
SKU: AB236321
Price: 525 USD
Availability: InStock

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(8 Publications)

Rabbit Polyclonal SMAD4 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SMAD4 aa 1-200.

View Alternative Names

DPC4, MADH4, SMAD4, SMAD family member 4, SMAD 4, hSMAD4, Deletion target in pancreatic carcinoma 4, Mothers against decapentaplegic homolog 4, MAD homolog 4, Mothers against DPP homolog 4

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody (AB236321)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody (AB236321)

Paraffin-embedded human kidney tissue stained for Smad4 using ab236321 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody (AB236321)

Paraffin-embedded human colon cancer tissue stained for Smad4 using ab236321 at 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human SMAD4 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q13485

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/200 - 1/2000", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Protein >95%.

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Smad4 also known as DPC4 or MADH4 is a central protein in the TGF-beta signaling pathway with a molecular mass of approximately 60 kDa. It acts as a signal transducer that facilitates communication from the cell surface to the nucleus. Smad4 is broadly expressed in various tissues playing an important role in the regulation of cellular processes. It forms a complex with receptor-regulated Smads (R-Smads) to translocate to the nucleus where it influences gene transcription.

Biological function summary

Smad4 influences cell proliferation differentiation and apoptosis by mediating signals from TGF-beta cytokines. It is part of the Smad protein family acting as a transcriptional controller. Upon TGF-beta receptor activation Smad4 forms complexes with Smad2 and Smad3 translocating to the nucleus to regulate genes imperative for cellular homeostasis. Its role in cell cycle regulation underlines its contribution to normal cellular functions and its potential involvement in disorders.

Pathways

Smad4 operates within the TGF-beta pathway linking extracellular signals to nuclear transcription alterations. It participates in the regulation of epithelial-mesenchymal transition (EMT) a process important for development and tumor progression. In these pathways Smad4 interacts closely with Smad2 and Smad3 orchestrating various cellular responses to external stimuli through transcriptional management.

Smad4 is highly related to cancer and juvenile polyposis syndrome. Mutations or deletions in Smad4 disrupt its function contributing to the progression of pancreatic cancer and colorectal cancer among others. Within these contexts Smad4 connects strongly to other proteins like p21 and cyclin-dependent kinase inhibitors which are important in cell cycle arrest and impede tumor growth.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (By similarity). Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling (PubMed : 25514493). Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

See full target information SMAD4

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Cancer reports (Hoboken, N.J.) :e1976 PubMed38230565

2024

SMAD4 inhibits glycolysis in ovarian cancer through PI3K/AKT/HK2 signaling pathway by activating ARHGAP10.

Applications

Unspecified application

Species

Unspecified reactive species

Kui Wu,Wei Gong,Huanmei Sun,Wenjiao Li,Li Chen,Yingchun Duan,Jianlong Zhu,Hu Zhang,Huihui Ke

American journal of translational research 15:151-164 PubMed36777827

2023

Protocatechuic acid, the main effective monomer in Wuqi Powder, can inhibit gastric ulcers induced by acetic acid and .

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Wang,Liang Zheng

Current issues in molecular biology 44:5048-5066 PubMed36286058

2022

Protective Effects of One 2,4-Dihydro-3H-Pyrazol-3-one Derivative against Posterior Capsular Opacification by Regulation of TGF-β2/SMADs and Non-SMAD Signaling, Collagen I, and Fibronectin Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Ching Shih,Chia-Yi Lee,Fung-Fuh Wong,Cheng-Hsiu Lin

American journal of translational research 13:659-671 PubMed33594316

2021

Berberine protects human and rat cardiomyocytes from hypoxia/reoxygenation-triggered apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Yang,Xu Shang,Guoqing Zhong,Liangli Hong,Zhi Li,Wanling Zhuang,Jidong Cheng

Cell cycle (Georgetown, Tex.) 18:2860-2875 PubMed31599708

2019

Overexpressed VEPH1 inhibits epithelial-mesenchymal transition, invasion, and migration of human cutaneous melanoma cells through inactivating the TGF-β signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Feng,Xiao-Min Jia,Ni-Na Gao,Hua Tang,Wei Huang,Ning Ning

Medicina (Kaunas, Lithuania) 55: PubMed31238579

2019

Prognostic Impact of Canonical TGF-β Signaling in Urothelial Bladder Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Slavica Stojnev,Miljan Krstić,Jovana Čukuranović Kokoris,Irena Conić,Ivan Petković,Sonja Ilić,Jelena Milosević-Stevanović,Ljubinka Janković Veličković

Journal of cellular physiology 234:20275-20285 PubMed30953355

2019

A novel mechanism of Smads/miR-675/TGFβR1 axis modulating the proliferation and remodeling of mouse cardiac fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Wang,Ping Jiang,Yi He,Hongyu Hu,Yuan Guo,Xiangyang Liu,Haihua Qiu,Qilin Ma,Fan Ouyang

Oncogenesis 8:1 PubMed30631034

2019

Inhibition of HGF/MET signaling decreases overall tumor burden and blocks malignant conversion in Tpl2-related skin cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Nicole F Bonan,David Kowalski,Kaitie Kudlac,Kira Flaherty,J Curtis Gwilliam,Lauren G Falkenberg,Erik Maradiaga,Kathleen L DeCicco-Skinner

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Smad4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody (AB236321)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody (AB236321)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise