Rabbit Recombinant Monoclonal SMAD4 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 21 publications.
Images
![Western blot - Anti-Smad4 antibody [EP618Y] - BSA and Azide free (AB215968), expandable thumbnail](https://content.abcam.com/products/images/smad4-antibody-ep618y-bsa-and-azide-free-ab215968--western-blot-img137610.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Smad4 antibody [EP618Y] - BSA and Azide free (AB215968)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [EP618Y] - BSA and Azide free (AB215968), expandable thumbnail](https://content.abcam.com/products/images/smad4-antibody-ep618y-bsa-and-azide-free-ab215968--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131673.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [EP618Y] - BSA and Azide free (AB215968)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [EP618Y] - BSA and Azide free (AB215968), expandable thumbnail](https://content.abcam.com/products/images/smad4-antibody-ep618y-bsa-and-azide-free-ab215968--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img45115.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [EP618Y] - BSA and Azide free (AB215968)")
![Western blot - Anti-Smad4 antibody [EP618Y] - BSA and Azide free (AB215968), expandable thumbnail](https://content.abcam.com/products/images/smad4-antibody-ep618y-bsa-and-azide-free-ab215968--western-blot-img167290.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Smad4 antibody [EP618Y] - BSA and Azide free (AB215968)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [EP618Y] - BSA and Azide free (AB215968), expandable thumbnail](https://content.abcam.com/products/images/smad4-antibody-ep618y-bsa-and-azide-free-ab215968--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img139907.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [EP618Y] - BSA and Azide free (AB215968)")
Publications
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- International journal of molecular sciences 21:2020RAP-011 Rescues the Disease Phenotype in a Cellular Model of Congenital Dyserythropoietic Anemia Type II by Inhibiting the SMAD2-3 Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGianluca De Rosa et. al.PubMed 32759740
- Cancer letters 360:227-332015Metabolic tumor burden is associated with major oncogenomic alterations and serum tumor markers in patients with resected pancreatic cancer.
- World journal of gastroenterology 20:14895-9032014Effect of oridonin-mediated hallmark changes on inflammatory pathways in human pancreatic cancer (BxPC-3) cells.Applications:WBReactive species:HumanRu-Yi Chen,Bin Xu,Su-Feng Chen,Si-Si Chen,Ting Zhang,Jun Ren,Jian XuPubMed 25356049
- Medical oncology (Northwood, London, England) 31:2362014The prognostic significance of Smad3, Smad4, Smad3 phosphoisoform expression in esophageal squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSoo Youn Cho et. al.PubMed 25267569
- Oncogene 34:3273-822014TGF-β signaling alters the pattern of liver tumorigenesis induced by Pten inactivation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesS M Morris et. al.PubMed 25132272
- Cancer biology & therapy 14:1059-672013Targeting the ERK pathway reduces liver metastasis of Smad4-inactivated colorectal cancer.
- Nature genetics 45:739-7462013Zbtb7a suppresses prostate cancer through repression of a Sox9-dependent pathway for cellular senescence bypass and tumor invasion.
- Journal of the American Society of Nephrology : JA 24:1387-982013Kindlin-2 mediates activation of TGF-β/Smad signaling and renal fibrosis.
- Cellular signalling 25:1981-922013Transforming growth factor β induces expression of connective tissue growth factor in hepatic progenitor cells through Smad independent signaling.
- British journal of cancer 108:1659-672013microRNA-183 is an oncogene targeting Dkk-3 and SMAD4 in prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesK Ueno et. al.PubMed 23538390
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Tested | Not recommended | Not recommended | Tested | Not recommended |
| Rat | Tested | Not recommended | Not recommended | Tested | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
3 products for Alternative Version
Target data
Function
In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (By similarity). Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling (PubMed:25514493). Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Alternative names
DPC4, MADH4, SMAD4, Mothers against decapentaplegic homolog 4, MAD homolog 4, Mothers against DPP homolog 4, Deletion target in pancreatic carcinoma 4, SMAD family member 4, SMAD 4, Smad4, hSMAD4
Recommended products
Rabbit Recombinant Monoclonal SMAD4 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 21 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP618Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab215968 is the carrier-free version of Anti-Smad4 antibody [EP618Y] ab40759.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Smad4 also known as DPC4 or MADH4 is a central protein in the TGF-beta signaling pathway with a molecular mass of approximately 60 kDa. It acts as a signal transducer that facilitates communication from the cell surface to the nucleus. Smad4 is broadly expressed in various tissues playing an important role in the regulation of cellular processes. It forms a complex with receptor-regulated Smads (R-Smads) to translocate to the nucleus where it influences gene transcription.
- Biological function summary
Smad4 influences cell proliferation differentiation and apoptosis by mediating signals from TGF-beta cytokines. It is part of the Smad protein family acting as a transcriptional controller. Upon TGF-beta receptor activation Smad4 forms complexes with Smad2 and Smad3 translocating to the nucleus to regulate genes imperative for cellular homeostasis. Its role in cell cycle regulation underlines its contribution to normal cellular functions and its potential involvement in disorders.
- Pathways
Smad4 operates within the TGF-beta pathway linking extracellular signals to nuclear transcription alterations. It participates in the regulation of epithelial-mesenchymal transition (EMT) a process important for development and tumor progression. In these pathways Smad4 interacts closely with Smad2 and Smad3 orchestrating various cellular responses to external stimuli through transcriptional management.
- Associated diseases and disorders
Smad4 is highly related to cancer and juvenile polyposis syndrome. Mutations or deletions in Smad4 disrupt its function contributing to the progression of pancreatic cancer and colorectal cancer among others. Within these contexts Smad4 connects strongly to other proteins like p21 and cyclin-dependent kinase inhibitors which are important in cell cycle arrest and impede tumor growth.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-Smad4 antibody [EP618Y] - BSA and Azide free (ab215968), expandable thumbnail](https://content.abcam.com/products/images/smad4-antibody-ep618y-bsa-and-azide-free-ab215968--western-blot-img137610.jpg "Western blot - Anti-Smad4 antibody [EP618Y] - BSA and Azide free (ab215968)")
Western blot - Anti-Smad4 antibody [EP618Y] - BSA and Azide free (ab215968)
This WB data was generated using the same anti-Smad4 antibody clone, EP618Y, in a different buffer formulation (cat# Anti-Smad4 antibody [EP618Y] ab40759).
Lane 1: Wild type HAP1 whole cell lysate (20 μg)
Lane 2: SMAD4 knockout HAP1 whole cell lysate (20 μg)
Lane 3: HepG2 whole cell lysate (20 μg)
Lane 4: Jurkat whole cell lysate (20 μg)Lanes 1 - 4: Merged signal (red and green). Green - Anti-Smad4 antibody [EP618Y] ab40759 observed at 60 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.
Anti-Smad4 antibody [EP618Y] ab40759 was shown to specifically react with SMAD4 in wild type HAP1 cells. No band was observed when SMAD4 knockout HAP1 samples were used. Wild-type and SMAD4 knockout samples were subjected to SDS-PAGE. Anti-Smad4 antibody [EP618Y] ab40759 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Smad4 antibody [EP618Y] (Anti-Smad4 antibody [EP618Y] ab40759)
Predicted band size: 60 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [EP618Y] - BSA and Azide free (ab215968), expandable thumbnail](https://content.abcam.com/products/images/smad4-antibody-ep618y-bsa-and-azide-free-ab215968--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131673.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [EP618Y] - BSA and Azide free (ab215968)")
This image is courtesy of an anonymous customer review.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [EP618Y] - BSA and Azide free (ab215968)
4% PFA-fixed, paraffin-embedded postnatal day 21 mouse ovary tissue stained for Smad4 using Anti-Smad4 antibody [EP618Y] ab40759 at 1/500 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Smad4 antibody [EP618Y] ab40759).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [EP618Y] - BSA and Azide free (ab215968), expandable thumbnail](https://content.abcam.com/products/images/smad4-antibody-ep618y-bsa-and-azide-free-ab215968--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img45115.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [EP618Y] - BSA and Azide free (ab215968)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [EP618Y] - BSA and Azide free (ab215968)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling Smad4 with unpurified Anti-Smad4 antibody [EP618Y] ab40759 at a 1/100 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Smad4 antibody [EP618Y] ab40759).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Western blot - Anti-Smad4 antibody [EP618Y] - BSA and Azide free (ab215968), expandable thumbnail](https://content.abcam.com/products/images/smad4-antibody-ep618y-bsa-and-azide-free-ab215968--western-blot-img167290.jpg "Western blot - Anti-Smad4 antibody [EP618Y] - BSA and Azide free (ab215968)")
Western blot - Anti-Smad4 antibody [EP618Y] - BSA and Azide free (ab215968)
This data was developed using Anti-Smad4 antibody [EP618Y] ab40759, the same antibody clone in a different buffer formulation. Different batches of Anti-Smad4 antibody [EP618Y] ab40759 were tested on Ramos (Human Burkitt's lymphoma B lymphocyte) lysate at 0.7 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 60 kDa.
All lanes: Western blot - Anti-Smad4 antibody [EP618Y] (Anti-Smad4 antibody [EP618Y] ab40759)
Predicted band size: 60 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [EP618Y] - BSA and Azide free (ab215968), expandable thumbnail](https://content.abcam.com/products/images/smad4-antibody-ep618y-bsa-and-azide-free-ab215968--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img139907.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [EP618Y] - BSA and Azide free (ab215968)")
This image is courtesy of an anonymous customer review.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [EP618Y] - BSA and Azide free (ab215968)
Unpurified Anti-Smad4 antibody [EP618Y] ab40759 staining Smad4 in rat femur tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 20 minutes at 22°C; antigen retrieval was by heat mediation in a citrate buffer pH6.0. Samples were incubated with primary antibody (1/200 in blocking buffer) for 2 hours at 20°C. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Smad4 antibody [EP618Y] ab40759).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [EP618Y] - BSA and Azide free (ab215968), expandable thumbnail](https://content.abcam.com/products/images/smad4-antibody-ep618y-bsa-and-azide-free-ab215968--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132709.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [EP618Y] - BSA and Azide free (ab215968)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [EP618Y] - BSA and Azide free (ab215968)
This IHC data was generated using the same anti-Smad4 antibody clone, EP618Y, in a different buffer formulation (cat# Anti-Smad4 antibody [EP618Y] ab40759).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Smad4 with purified Anti-Smad4 antibody [EP618Y] ab40759 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [EP618Y] - BSA and Azide free (ab215968), expandable thumbnail](https://content.abcam.com/products/images/smad4-antibody-ep618y-bsa-and-azide-free-ab215968--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132709.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [EP618Y] - BSA and Azide free (ab215968)")
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