Rabbit Recombinant Monoclonal SMAD4 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), ChIC/CUT&RUN-seq and reacts with Mouse, Human, Rat samples.
Images
![Immunoprecipitation - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (AB254290), expandable thumbnail](https://content.abcam.com/products/images/smad4-antibody-epr22589-112-bsa-and-azide-free-ab254290--immunoprecipitation-img8699.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (AB254290)")
![Western blot - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (AB254290), expandable thumbnail](https://content.abcam.com/products/images/smad4-antibody-epr22589-112-bsa-and-azide-free-ab254290--western-blot-img61563.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (AB254290)")
![Immunocytochemistry/ Immunofluorescence - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (AB254290), expandable thumbnail](https://content.abcam.com/products/images/smad4-antibody-epr22589-112-bsa-and-azide-free-ab254290--immunocytochemistry-immunofluorescence-img63475.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (AB254290)")
![Flow Cytometry (Intracellular) - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (AB254290), expandable thumbnail](https://content.abcam.com/products/images/smad4-antibody-epr22589-112-bsa-and-azide-free-ab254290--flow-cytometry-intracellular-img37286.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (AB254290)")
![Immunoprecipitation - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (AB254290), expandable thumbnail](https://content.abcam.com/products/images/smad4-antibody-epr22589-112-bsa-and-azide-free-ab254290--immunoprecipitation-img8775.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (AB254290)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | ChIP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | ChIC/CUT&RUN-seq | |
|---|---|---|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Not recommended | Tested | Tested | Tested |
| Mouse | Tested | Not recommended | Tested | Not recommended | Expected | Expected | Expected |
| Rat | Expected | Not recommended | Tested | Not recommended | Expected | Expected | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ChIP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ChIC/CUT&RUN-seq
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (By similarity). Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling (PubMed:25514493). Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Alternative names
DPC4, MADH4, SMAD4, Mothers against decapentaplegic homolog 4, MAD homolog 4, Mothers against DPP homolog 4, Deletion target in pancreatic carcinoma 4, SMAD family member 4, SMAD 4, Smad4, hSMAD4
Recommended products
Rabbit Recombinant Monoclonal SMAD4 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), ChIC/CUT&RUN-seq and reacts with Mouse, Human, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR22589-112
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab254290 is the carrier-free version of Anti-Smad4 antibody [EPR22589-112] ab230815.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Smad4 also known as DPC4 or MADH4 is a central protein in the TGF-beta signaling pathway with a molecular mass of approximately 60 kDa. It acts as a signal transducer that facilitates communication from the cell surface to the nucleus. Smad4 is broadly expressed in various tissues playing an important role in the regulation of cellular processes. It forms a complex with receptor-regulated Smads (R-Smads) to translocate to the nucleus where it influences gene transcription.
- Biological function summary
Smad4 influences cell proliferation differentiation and apoptosis by mediating signals from TGF-beta cytokines. It is part of the Smad protein family acting as a transcriptional controller. Upon TGF-beta receptor activation Smad4 forms complexes with Smad2 and Smad3 translocating to the nucleus to regulate genes imperative for cellular homeostasis. Its role in cell cycle regulation underlines its contribution to normal cellular functions and its potential involvement in disorders.
- Pathways
Smad4 operates within the TGF-beta pathway linking extracellular signals to nuclear transcription alterations. It participates in the regulation of epithelial-mesenchymal transition (EMT) a process important for development and tumor progression. In these pathways Smad4 interacts closely with Smad2 and Smad3 orchestrating various cellular responses to external stimuli through transcriptional management.
- Associated diseases and disorders
Smad4 is highly related to cancer and juvenile polyposis syndrome. Mutations or deletions in Smad4 disrupt its function contributing to the progression of pancreatic cancer and colorectal cancer among others. Within these contexts Smad4 connects strongly to other proteins like p21 and cyclin-dependent kinase inhibitors which are important in cell cycle arrest and impede tumor growth.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunoprecipitation - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (ab254290), expandable thumbnail](https://content.abcam.com/products/images/smad4-antibody-epr22589-112-bsa-and-azide-free-ab254290--immunoprecipitation-img8699.jpg "Immunoprecipitation - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (ab254290)")
Immunoprecipitation - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (ab254290)
Smad4 was immunoprecipitated from 0.35mg of HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with Anti-Smad4 antibody [EPR22589-112] ab230815 at 1/30 dilution. Western Blot was performed from the immunoprecipitate using Anti-Smad4 antibody [EPR22589-112] ab230815 at 1/1000 dilution (0.5 μg/ml).
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used as secondary antibody at 1/5000 dilution.
Lane 1: HeLa whole cell lysate 10μg (Input).
Lane 2: Anti-Smad4 antibody [EPR22589-112] ab230815 IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Smad4 antibody [EPR22589-112] ab230815 in HeLa whole cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 30 seconds
Lysate was freshly prepared and IP test was done immediately to avoid protein degradation. Lysate incubation time was shortened from overnight to 2h.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Smad4 antibody [EPR22589-112] ab230815).
All lanes: Immunoprecipitation - Anti-Smad4 antibody [EPR22589-112] (Anti-Smad4 antibody [EPR22589-112] ab230815)
Predicted band size: 60 kDa
![Western blot - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (ab254290), expandable thumbnail](https://content.abcam.com/products/images/smad4-antibody-epr22589-112-bsa-and-azide-free-ab254290--western-blot-img61563.jpg "Western blot - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (ab254290)")
Western blot - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (ab254290)
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure Time: Lanes 1-6: 15 secs; Lanes 7-8: 114 secs; Lane 9: 3 mins.
Anti-Smad4 antibody [EPR22589-112] ab230815 was shown to specifically react with Smad4 in wild-type HAP1 cells as signal was lost in Smad4 knockout cells. Wild-type and Smad4 knockout samples were subjected to SDS-PAGE. Anti-Smad4 antibody [EPR22589-112] ab230815 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Smad4 antibody [EPR22589-112] ab230815).
All lanes: Western blot - Anti-Smad4 antibody [EPR22589-112] (Anti-Smad4 antibody [EPR22589-112] ab230815) at 1/1000 dilution
Lane 1: Smad4 knockout HAP1 whole cell lysate at 20 µg
Lane 2: Wild-type HAP1 whole cell lysate at 20 µg
Lane 3: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 4: HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 5: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 6: Human fetal lung tissue lysate at 20 µg
Lane 7: Mouse embryo tissue lysate at 20 µg
Lane 8: Mouse lung tissue lysate at 20 µg
Lane 9: Rat lung tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (ab254290), expandable thumbnail](https://content.abcam.com/products/images/smad4-antibody-epr22589-112-bsa-and-azide-free-ab254290--immunocytochemistry-immunofluorescence-img63475.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (ab254290)")
Immunocytochemistry/ Immunofluorescence - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (ab254290)
Immunofluorescent analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling Smad4 with Anti-Smad4 antibody [EPR22589-112] ab230815 at a 1/100 dilution (green). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Confocal image showing mainly nuclear staining in HeLa cell line treated with TGF-beta (10ng/ml) for 1 h. AlexaFluor®488 Goat anti-Rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used secondary antibody at 1/1000 dilution, this was also used on its own as a control. DAPI was used as a nuclear counterstain (blue). Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594, Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) was used as a counterstain at 1/200 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Smad4 antibody [EPR22589-112] ab230815).
![Flow Cytometry (Intracellular) - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (ab254290), expandable thumbnail](https://content.abcam.com/products/images/smad4-antibody-epr22589-112-bsa-and-azide-free-ab254290--flow-cytometry-intracellular-img37286.jpg "Flow Cytometry (Intracellular) - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (ab254290)")
Flow Cytometry (Intracellular) - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (ab254290)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed Smad4 KO HAP1 (Smad4 knockout human chronic myelogenous leukemia near-haploid cell line, Left) / WT HAP1 (human chronic myelogenous leukemia near-haploid cell line, Right) cell line labeling Smad4 with Anti-Smad4 antibody [EPR22589-112] ab230815 at 1/500 (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as secondary antibody at 1/2000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Smad4 antibody [EPR22589-112] ab230815).
![Immunoprecipitation - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (ab254290), expandable thumbnail](https://content.abcam.com/products/images/smad4-antibody-epr22589-112-bsa-and-azide-free-ab254290--immunoprecipitation-img8775.jpg "Immunoprecipitation - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (ab254290)")
Immunoprecipitation - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (ab254290)
Smad4 was immunoprecipitated from 0.35mg of NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with Anti-Smad4 antibody [EPR22589-112] ab230815 at 1/30 dilution. Western Blot was performed from the immunoprecipitate using Anti-Smad4 antibody [EPR22589-112] ab230815 at 1/1000 dilution (0.5 μg/ml).
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used as secondary antibody at 1/5000 dilution.
Lane 1: NIH/3T3 whole cell lysate 10μg (Input).
Lane 2: Anti-Smad4 antibody [EPR22589-112] ab230815 IP in NIH/3T3 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Smad4 antibody [EPR22589-112] ab230815 in NIH/3T3 whole cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 10 seconds
Lysate was freshly prepared and IP test was done immediately to avoid protein degradation. Lysate incubation time was shortened from overnight to 2h.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Smad4 antibody [EPR22589-112] ab230815).
All lanes: Immunoprecipitation - Anti-Smad4 antibody [EPR22589-112] (Anti-Smad4 antibody [EPR22589-112] ab230815)
Predicted band size: 60 kDa
![ChIC/CUT&RUN sequencing - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (ab254290), expandable thumbnail](https://content.abcam.com/products/images/smad4-antibody-epr22589-112-bsa-and-azide-free-ab254290--chic-cut-run-sequencing-img439410.jpg "ChIC/CUT&RUN sequencing - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (ab254290)")
ChIC/CUT&RUN sequencing - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (ab254290)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Smad4 antibody [EPR22589-112] ab230815).
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 105 HeLa (Human epithelial cell line from cervix adenocarcinoma) cells treated with hTGF-β1 (10 ng/mL 1 h) and 5 µg of Anti-Smad4 antibody [EPR22589-112] ab230815 [EPR22589-112]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
![ChIC/CUT&RUN sequencing - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (ab254290), expandable thumbnail](https://content.abcam.com/products/images/smad4-antibody-epr22589-112-bsa-and-azide-free-ab254290--chic-cut-run-sequencing-img439411.jpg "ChIC/CUT&RUN sequencing - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (ab254290)")
ChIC/CUT&RUN sequencing - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (ab254290)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Smad4 antibody [EPR22589-112] ab230815).
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 105 HeLa (Human epithelial cell line from cervix adenocarcinoma) cells treated with hTGF-β1 (10 ng/mL 1 h) and 5 µg of Anti-Smad4 antibody [EPR22589-112] ab230815 [EPR22589-112]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
![ChIC/CUT&RUN sequencing - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (ab254290), expandable thumbnail](https://content.abcam.com/products/images/smad4-antibody-epr22589-112-bsa-and-azide-free-ab254290--chic-cut-run-sequencing-img439412.jpg "ChIC/CUT&RUN sequencing - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (ab254290)")
ChIC/CUT&RUN sequencing - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (ab254290)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Smad4 antibody [EPR22589-112] ab230815).
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 105 HeLa (Human epithelial cell line from cervix adenocarcinoma) cells treated with hTGF-β1 (10 ng/mL 1 h) and 5 µg of Anti-Smad4 antibody [EPR22589-112] ab230815 [EPR22589-112]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
![ChIC/CUT&RUN sequencing - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (ab254290), expandable thumbnail](https://content.abcam.com/products/images/smad4-antibody-epr22589-112-bsa-and-azide-free-ab254290--chic-cut-run-sequencing-img439410.jpg/_jcr_content/renditions/webp-475.webp "ChIC/CUT&RUN sequencing - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (ab254290)")
![ChIC/CUT&RUN sequencing - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (ab254290), expandable thumbnail](https://content.abcam.com/products/images/smad4-antibody-epr22589-112-bsa-and-azide-free-ab254290--chic-cut-run-sequencing-img439411.jpg/_jcr_content/renditions/webp-475.webp "ChIC/CUT&RUN sequencing - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (ab254290)")
![ChIC/CUT&RUN sequencing - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (ab254290), expandable thumbnail](https://content.abcam.com/products/images/smad4-antibody-epr22589-112-bsa-and-azide-free-ab254290--chic-cut-run-sequencing-img439412.jpg/_jcr_content/renditions/webp-475.webp "ChIC/CUT&RUN sequencing - Anti-Smad4 antibody [EPR22589-112] - BSA and Azide free (ab254290)")
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