AB80049

Anti-SMAD6 antibody

Name: Anti-SMAD6 antibody (ab80049) | Abcam
Brand: Abcam Limited
SKU: AB80049
Price: 515 USD
Availability: InStock

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(10 Publications)

Rabbit Polyclonal SMAD6 antibody. Suitable for WB and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human SMAD6.

View Alternative Names

MADH6, SMAD6, Mothers against decapentaplegic homolog 6, MAD homolog 6, Mothers against DPP homolog 6, SMAD family member 6, SMAD 6, Smad6, hSMAD6

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human SMAD6. The exact immunogen used to generate this antibody is proprietary information.

O43541

Specificity

This reacts specifically with human 54 kDa SMAD6 isoform D protein. It is not expected to cross react with other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Constituents: Whole serum

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMAD6 also known as MADH6 or SMAD family member 6 is an inhibitory regulator in the TGF-beta signaling pathway. It has a molecular mass of approximately 53 kDa. SMAD6 specifically binds to and inhibits the BMP (Bone Morphogenetic Protein) receptor-activated SMAD proteins mainly SMAD1 SMAD5 and SMAD8 preventing their phosphorylation and subsequent translocation into the nucleus. This protein is expressed in various tissues including the heart skeletal muscle and brain.

Biological function summary

SMAD6 plays a critical role in modulating BMP signaling. It functions not as part of a larger protein complex but as a direct antagonist to BMP-induced cellular responses. By binding to SMAD1 and SMAD5 SMAD6 impedes the transcriptional activity that promotes various cellular processes such as proliferation differentiation and apoptosis. This regulation is essential for maintaining cellular homeostasis and ensuring proper embryonic development.

Pathways

SMAD6 serves as an important checkpoint within the TGF-beta/BMP signaling pathway. It relates to other proteins such as SMAD7 which also functions as an inhibitory SMAD although SMAD6 is more selective in antagonizing BMP receptors. Through its actions SMAD6 aids in the regulation of processes like bone formation and vascular development. By throttling the BMP pathway SMAD6 maintains a balance between proliferative signals and inhibitory cues important for organogenesis.

Disrupted SMAD6 function associates with cardiovascular and skeletal abnormalities. Mutations or altered expression levels in SMAD6 have links to congenital heart defects where it fails to adequately regulate BMP signaling. This dysregulation can also involve proteins such as SMAD1 leading to improper cardiac development. Additionally SMAD6 might affect conditions like fibrodysplasia ossificans progressiva that feature abnormal bone growth further implicating the need for balanced BMP signaling in maintaining tissue integrity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transforming growth factor-beta superfamily receptors signaling occurs through the Smad family of intracellular mediators. SMAD6 is an inhibitory Smad (i-Smad) that negatively regulates signaling downstream of type I transforming growth factor-beta (PubMed : 10647776, PubMed : 10708948, PubMed : 10708949, PubMed : 16951688, PubMed : 22275001, PubMed : 30848080, PubMed : 9436979, PubMed : 9759503). Acts as a mediator of TGF-beta and BMP anti-inflammatory activities. Suppresses IL1R-TLR signaling through its direct interaction with PEL1, preventing NF-kappa-B activation, nuclear transport and NF-kappa-B-mediated expression of pro-inflammatory genes (PubMed : 16951688). Blocks the BMP-SMAD1 signaling pathway by competing with SMAD4 for receptor-activated SMAD1-binding (PubMed : 30848080, PubMed : 9436979). Binds to regulatory elements in target promoter regions (PubMed : 16491121).

See full target information SMAD6

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:670145 PubMed34079802

2021

The Inhibition of B7H3 by 2-HG Accumulation Is Associated With Downregulation of VEGFA in IDH Mutated Gliomas.

Applications

Unspecified application

Species

Unspecified reactive species

Mengli Zhang,Huaichao Zhang,Minjie Fu,Jingwen Zhang,Cheng Zhang,Yingying Lv,Fengfeng Fan,Jinsen Zhang,Hao Xu,Dan Ye,Hui Yang,Wei Hua,Ying Mao

eLife 9: PubMed33006314

2020

GAS5 protects against osteoporosis by targeting UPF1/SMAD7 axis in osteoblast differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Li,Zhongyu Xie,Jinteng Li,Jiajie Lin,Guan Zheng,Wenjie Liu,Su'an Tang,Shuizhong Cen,Guiwen Ye,Zhaofeng Li,Wenhui Yu,Peng Wang,Yanfeng Wu,Huiyong Shen

Cancer cell international 20:360 PubMed32774154

2020

Circular RNA hsa_circ_0000517 modulates hepatocellular carcinoma advancement via the miR-326/SMAD6 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Shuwei He,Zhengwu Guo,Qian Kang,Xu Wang,Xingmin Han

Biochemistry research international 2020:6285987 PubMed32802507

2020

Construction and Investigation of MicroRNA-mRNA Regulatory Network of Gastric Cancer with Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Yang,Junjie Liu,Tianci Yang,Lei Zhang,Peiyou Gong,Boqing Li,Xiuzhi Zhou

Journal of nanobiotechnology 18:68 PubMed32375794

2020

Saliva exosomes-derived UBE2O mRNA promotes angiogenesis in cutaneous wounds by targeting SMAD6.

Applications

Unspecified application

Species

Unspecified reactive species

Bobin Mi,Lang Chen,Yuan Xiong,Chenchen Yan,Hang Xue,Adriana C Panayi,Jing Liu,Liangcong Hu,Yiqiang Hu,Faqi Cao,Yun Sun,Wu Zhou,Guohui Liu

Molecular therapy. Nucleic acids 19:827-840 PubMed31981860

2020

Overexpressed lncRNA GATA6-AS1 Inhibits LNM and EMT via FZD4 through the Wnt/β-Catenin Signaling Pathway in GC.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng-Tian Li,Xu Zhang,Da-Wei Wang,Jun Xu,Ke-Jian Kou,Zhi-Wei Wang,Gong Yong,De-Sen Liang,Xue-Ying Sun

Cancer cell international 20:23 PubMed31992960

2020

SMAD6, positively regulated by the DNM3OS-miR-134-5p axis, confers promoting effects to cell proliferation, migration and EMT process in retinoblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Wang,Xiang Ji

International journal of molecular sciences 20: PubMed31690042

2019

RON Receptor Tyrosine Kinase Regulates Epithelial Mesenchymal Transition and the Expression of Pro-Fibrotic Markers via Src/Smad Signaling in HK-2 and NRK49F Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jung Sun Park,Hoon-In Choi,Dong-Hyun Kim,Chang Seong Kim,Eun Hui Bae,Seong Kwon Ma,Soo Wan Kim

Bioscience reports 39: PubMed30135141

2019

miR-134 inhibits chondrogenic differentiation of bone marrow mesenchymal stem cells by targetting SMAD6.

Applications

Unspecified application

Species

Unspecified reactive species

Shaogang Xu,Xuejian Wu

PLoS biology 16:e2004425 PubMed29949573

2018

Intracellular attenuation of BMP signaling via CKIP-1/Smurf1 is essential during neural crest induction.

Applications

Unspecified application

Species

Unspecified reactive species

Michael L Piacentino,Marianne E Bronner

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SMAD6 antibody

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

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