AB283282

Anti-SMAD6 antibody [EPR24687-21] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal SMAD6 antibody. Carrier free. Suitable for WB and reacts with Mouse, Human samples.

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Lab

Western blot - Anti-SMAD6 antibody [EPR24687-21] - BSA and Azide free (AB283282)

This data was developed using ab273106, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST. The observed MW & expression profile are consistent with what has been described in the literature (PMID : 19047146; PMID : 9256479).

NCI-H460 was reported for low-abundance for the dominant 62kDa isoform of SMAD6(PMID : 19047146).

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-SMAD6 antibody [EPR24687-21] (<a href='/en-us/products/primary-antibodies/smad6-antibody-epr24687-21-ab273106'>ab273106</a>) at 1/1000 dilution

Lane 1:

NCI-H1299 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

A549 (human lu carcinoma epithelial cell) whole cell lysate 20 at 20 µg

Lane 3:

NCI-H460 (human Large Cell Lu Cancer epithelial cell) whole cell lysate 20 at 20 µg

Lane 4:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 5:

U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

Lane 6:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 53 kDa

Observed band size: 30 kDa

false

Unconjugated

Anti-SMAD6 antibody [EPR24687-21]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24687-21

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab283282 is the carrier-free version of ab273106.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMAD6 also known as MADH6 or SMAD family member 6 is an inhibitory regulator in the TGF-beta signaling pathway. It has a molecular mass of approximately 53 kDa. SMAD6 specifically binds to and inhibits the BMP (Bone Morphogenetic Protein) receptor-activated SMAD proteins mainly SMAD1 SMAD5 and SMAD8 preventing their phosphorylation and subsequent translocation into the nucleus. This protein is expressed in various tissues including the heart skeletal muscle and brain.

Biological function summary

SMAD6 plays a critical role in modulating BMP signaling. It functions not as part of a larger protein complex but as a direct antagonist to BMP-induced cellular responses. By binding to SMAD1 and SMAD5 SMAD6 impedes the transcriptional activity that promotes various cellular processes such as proliferation differentiation and apoptosis. This regulation is essential for maintaining cellular homeostasis and ensuring proper embryonic development.

Pathways

SMAD6 serves as an important checkpoint within the TGF-beta/BMP signaling pathway. It relates to other proteins such as SMAD7 which also functions as an inhibitory SMAD although SMAD6 is more selective in antagonizing BMP receptors. Through its actions SMAD6 aids in the regulation of processes like bone formation and vascular development. By throttling the BMP pathway SMAD6 maintains a balance between proliferative signals and inhibitory cues important for organogenesis.

Disrupted SMAD6 function associates with cardiovascular and skeletal abnormalities. Mutations or altered expression levels in SMAD6 have links to congenital heart defects where it fails to adequately regulate BMP signaling. This dysregulation can also involve proteins such as SMAD1 leading to improper cardiac development. Additionally SMAD6 might affect conditions like fibrodysplasia ossificans progressiva that feature abnormal bone growth further implicating the need for balanced BMP signaling in maintaining tissue integrity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transforming growth factor-beta superfamily receptors signaling occurs through the Smad family of intracellular mediators. SMAD6 is an inhibitory Smad (i-Smad) that negatively regulates signaling downstream of type I transforming growth factor-beta (PubMed : 10647776, PubMed : 10708948, PubMed : 10708949, PubMed : 16951688, PubMed : 22275001, PubMed : 30848080, PubMed : 9436979, PubMed : 9759503). Acts as a mediator of TGF-beta and BMP anti-inflammatory activities. Suppresses IL1R-TLR signaling through its direct interaction with PEL1, preventing NF-kappa-B activation, nuclear transport and NF-kappa-B-mediated expression of pro-inflammatory genes (PubMed : 16951688). Blocks the BMP-SMAD1 signaling pathway by competing with SMAD4 for receptor-activated SMAD1-binding (PubMed : 30848080, PubMed : 9436979). Binds to regulatory elements in target promoter regions (PubMed : 16491121).

See full target information SMAD6

Alternative Names

MADH6, SMAD6, SMAD family member 6, SMAD 6, hSMAD6, Mothers against decapentaplegic homolog 6, MAD homolog 6, Mothers against DPP homolog 6

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SMAD6 antibody [EPR24687-21] - BSA and Azide free

Western blot - Anti-SMAD6 antibody [EPR24687-21] - BSA and Azide free (AB283282)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Product promise