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AB262940

Anti-SMAD9 antibody

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Rabbit Polyclonal SMAD9 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SMAD9 aa 100 to C-terminus.

View Alternative Names

MADH6, MADH9, SMAD8, SMAD9, SMAD family member 9, SMAD 9, Madh6, Mothers against decapentaplegic homolog 9, MAD homolog 9, Mothers against DPP homolog 9

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMAD9 antibody (AB262940)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMAD9 antibody (AB262940)

Paraffin-embedded Human colon tissue stained for SMAD9 using ab262940 at 1/1000 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-SMAD9 antibody (AB262940)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SMAD9 antibody (AB262940)

PFA-fixed, Triton X-100 permeabilized human RH-30 cells stained for SMAD9 (green) using ab262940 at 4 µg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SMAD9 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O15198

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMAD9 also known as Mothers against decapentaplegic homolog 9 or MADH9 is a transcription factor involved in signal transduction and transcriptional activity. It has a molecular mass of approximately 50 kDa. SMAD9 is widely expressed in various tissues including the heart brain and skeletal muscle. In its mechanical function SMAD9 becomes activated through phosphorylation by type I receptor serine/threonine kinases and forms part of the SMAD protein family that transmits extracellular signals to the nucleus affecting gene expression.
Biological function summary

SMAD9 plays a role in the cellular processes of growth and differentiation particularly within the bone morphogenetic protein (BMP) signaling pathway. It operates as part of a complex with other SMAD proteins such as SMAD1 and SMAD5 forming heteromeric complexes that regulate the transcription of BMP-responsive genes. This gene transcription is vital for the development and repair of tissues as well as the maintenance of cellular homeostasis.

Pathways

SMAD9 participates significantly in the BMP and transforming growth factor-beta (TGF-β) signaling pathways. These pathways are important for a variety of cellular responses including proliferation apoptosis and differentiation. SMAD9 associates with BMP receptors to mediate the BMP signaling cascade acting synergistically with other SMAD family members like SMAD4 which is a common mediator in these pathways. This interrelation places SMAD9 at a pivotal point within the regulation of multiple cellular responses.

Research indicates that SMAD9 mutations or dysregulation can associate with pulmonary arterial hypertension (PAH) and certain forms of cancer. In PAH altered SMAD9 signaling disrupts normal vascular remodeling resulting in elevated pulmonary artery pressure. Additionally it can interact with other proteins like BMPR2 a receptor for BMP which also plays a significant role in the pathogenesis of PAH. Understanding the involvement of SMAD9 in these conditions can provide insights into potential therapeutic targets for treatment.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD9 is a receptor-regulated SMAD (R-SMAD).
See full target information SMAD9
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Product promise

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