Rabbit Polyclonal SMAP antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SMAP aa 1-50.
View Alternative Names
C11orf58, SMAP, Small acidic protein
- IP
Supplier Data
Immunoprecipitation - Anti-SMAP antibody (AB202282)
Lane 1 : Detection of Human SMAP by Immunoprecipitation from 293T whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using ab202282 at 6 μg per reaction for IP.
Lane 2 : Immunoprecipitation from 293T whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using an antibody which recognized an epitope of SMAP.
Lane 3 : Immunoprecipitation from 293T whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using Control IgG.
Subsequent Western blot detection used ab202282 at 1 μg/ml.
Detection : Chemiluminescence with an exposure time of 30
seconds.
All lanes:
Immunoprecipitation - Anti-SMAP antibody (ab202282)
Predicted band size: 20 kDa
true
Exposure time: 30s
- WB
Supplier Data
Western blot - Anti-SMAP antibody (AB202282)
All lanes:
Western blot - Anti-SMAP antibody (ab202282) at 0.1 µg/mL
Lane 1:
Whole cell lysate from HeLa cells at 50 µg
Lane 2:
Whole cell lysate from 293T cells at 50 µg
Lane 3:
Whole cell lysate from Jurkat cells at 50 µg
Lane 4:
Whole cell lysate from mouse TCMK-1 cells at 50 µg
Lane 5:
Whole cell lysate from mouse NIH 3T3 cells at 50 µg
Predicted band size: 20 kDa
true
Exposure time: 3min
Reactivity data
Properties and storage information
Form
Purification technique
Purification notes
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SMAP influences cellular processes by modulating membrane trafficking dynamics. SMAP operates as part of a complex involved in the regulation of vesicular transport. By assisting the hydrolysis of GTP bound to Arf proteins SMAP can control vesicle budding and transport at the Golgi apparatus. This function enables SMAP to participate actively in managing intracellular transport and membrane curvature events impacting cell signaling and other cellular activities.
Pathways
SMAP functions in the clathrin-mediated endocytosis pathway and the Golgi transport pathway. Through its activity SMAP links to the Arf family proteins which are components in these pathways. In clathrin-mediated endocytosis SMAP facilitates cargo selection and vesicle formation interacting with molecules like AP-2. SMAP's GAP activity is important in Golgi-associated vesicular trafficking where it regulates the cycle of Arf GTPases contributing to vesicle docking and fusion events.
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Target data
Publications (1)
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iScience 26:106380 PubMed37009223
2023
Applications
Unspecified application
Species
Unspecified reactive species
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