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AB227000

Anti-SMARCA2 / BRM antibody

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(1 Publication)

Rabbit Polyclonal SMARCA2 / BRM antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SMARCA2.

View Alternative Names

BAF190B, BRM, SNF2A, SNF2L2, SMARCA2, Probable global transcription activator SNF2L2, ATP-dependent helicase SMARCA2, BRG1-associated factor 190B, Protein brahma homolog, SNF2-alpha, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2, hBRM

7 Images
Immunocytochemistry/ Immunofluorescence - Anti-SMARCA2 / BRM antibody (AB227000)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SMARCA2 / BRM antibody (AB227000)

4% paraformaldehyde fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SMARCA2 / BRM using ab227000 at 1/500 dilution in ICC/IF.

Red : Phalloidin.

Immunocytochemistry/ Immunofluorescence - Anti-SMARCA2 / BRM antibody (AB227000)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SMARCA2 / BRM antibody (AB227000)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling SMARCA2 protein using ab227000 at a 1/500 dilution (Green). phalloidin, a cytoskeleton marker was stained at a 1/200 dilution (Red). Scale bar= 10µm.

Immunocytochemistry/ Immunofluorescence - Anti-SMARCA2 / BRM antibody (AB227000)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SMARCA2 / BRM antibody (AB227000)

Immunofluorescence analysis of DIV10 rat hippocampal neuron cells fixed in 4% paraformaldehyde at RT for 15 min labelling SMARCA2 protein using ab227000 at a 1/500 dilution (Green). Tau, a marker, stained by Tau antibody at a 1/500 dilution (Red).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCA2 / BRM antibody (AB227000)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCA2 / BRM antibody (AB227000)

Paraffin embedded mouse testis tissue stained for SMARCA2 / BRM using ab227000 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCA2 / BRM antibody (AB227000)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCA2 / BRM antibody (AB227000)

Paraffin embedded mouse brain tissue stained for SMARCA2 / BRM using ab227000 at 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-SMARCA2 / BRM antibody (AB227000)
  • WB

Supplier Data

Western blot - Anti-SMARCA2 / BRM antibody (AB227000)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-SMARCA2 / BRM antibody (ab227000) at 1/1000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Lane 3:

HeLa whole cell lysate at 30 µg

Lane 4:

HepG2 whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 181 kDa

false

Western blot - Anti-SMARCA2 / BRM antibody (AB227000)
  • WB

Supplier Data

Western blot - Anti-SMARCA2 / BRM antibody (AB227000)

Samples were separated by 5% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-SMARCA2 / BRM antibody (ab227000) at 1/1000 dilution

Lane 1:

A431 whole cell lysate at 30 µg

Lane 2:

HeLa whole cell lysate at 30 µg

Lane 3:

HepG2 whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 181 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human, Rat

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human SMARCA2. The exact immunogen used to generate this antibody is proprietary information.

P51531

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SMARCA2 protein also known as BRM (Brahma-related gene 1) is a component of the SWI/SNF chromatin remodeling complex. This protein has a molecular mass of approximately 185 kDa. Scientists observe that SMARCA2/BRM is expressed in various tissues including the brain and liver. It acts as an ATPase which moves histone octamers in chromatin and facilitates access to DNA. SMARCA2/BRM plays an important role in transcriptional activation by modifying chromatin structure.
Biological function summary

SMARCA2/BRM interacts with other subunits to form the SWI/SNF complex important for regulating gene expression. This complex helps control the transcription of specific genes by repositioning nucleosomes. SMARCA2/BRM modulates cell cycle progression and differentiation as it influences transcription factor access to DNA. Its activity is essential for normal cellular responses maintaining cell homeostasis and development.

Pathways

SMARCA2/BRM participates significantly in the cell cycle and Wnt signaling pathways. In the cell cycle it cooperates with proteins like p53 to regulate genes involved in cell growth and apoptosis. In the Wnt pathway SMARCA2/BRM interacts with beta-catenin to influence cellular responses to Wnt signals. These pathways are critical for cell proliferation and maintaining cellular integrity and SMARCA2/BRM serves as a pivotal modulator within these systems.

SMARCA2/BRM has a connection to cancer and Coffin-Siris syndrome. Altered expression of SMARCA2/BRM has been observed in several cancers where it frequently interacts with other oncogenic proteins disrupting normal regulatory functions and promoting tumorigenesis. Meanwhile mutations in SMARCA2 can lead to Coffin-Siris syndrome a genetic disorder affecting development and it is connected with other proteins like SOX2 which are also involved in developmental pathways. Understanding SMARCA2's role may uncover insights into therapeutic targets for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Binds DNA non-specifically (PubMed : 22952240, PubMed : 26601204). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity).
See full target information SMARCA2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 29:155 PubMed39911153

2025

Construction of a lung adenocarcinoma prognostic model based on KEAP1/NRF2/HO‑1 mutation‑mediated upregulated genes and bioinformatic analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhu,Ye Zhang,Lingyun Yang,Lu Chen,Chaobo Chen,Qifeng Shi,Zipeng Xu
View all publications
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