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Rabbit Recombinant Monoclonal SMARCA2 / BRM antibody. Suitable for WB, IP, ChIC/CUT&RUN-seq and reacts with Recombinant fragment - Human, Human samples.

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Images

Western blot - Anti-SMARCA2 / BRM antibody [EPR28611-92] (AB323179), expandable thumbnail
  • Western blot - Anti-SMARCA2 / BRM antibody [EPR28611-92] (AB323179), expandable thumbnail
  • Western blot - Anti-SMARCA2 / BRM antibody [EPR28611-92] (AB323179), expandable thumbnail
  • ChIC/CUT&RUN sequencing - Anti-SMARCA2 / BRM antibody [EPR28611-92] (AB323179), expandable thumbnail
  • ChIC/CUT&RUN sequencing - Anti-SMARCA2 / BRM antibody [EPR28611-92] (AB323179), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPChIC/CUT&RUN-seqChIP-seq
Human
Tested
Tested
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Recombinant fragment - Human
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Recombinant fragment - Human
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Mouse, Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
5 µg
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Recombinant fragment - Human, Mouse
Dilution info
-
Notes

-

Target data

Function

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Binds DNA non-specifically (PubMed:22952240, PubMed:26601204). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity).

Alternative names

Rabbit Recombinant Monoclonal SMARCA2 / BRM antibody. Suitable for WB, IP, ChIC/CUT&RUN-seq and reacts with Recombinant fragment - Human, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28611-92
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-SMARCA2 / BRM antibody [EPR28611-92] (ab323179), expandable thumbnail

    Western blot - Anti-SMARCA2 / BRM antibody [EPR28611-92] (ab323179)

    SMARCA2 / BRM Western blot staining using rabbit Anti-SMARCA2 / BRM antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: SW-13, NCCIT (PMID: 16007216).

    To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-SMARCA2 / BRM antibody [EPR28611-92] (ab323179) at 1/1000 dilution

    Lane 1: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 50 µg

    Lane 2: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 50 µg

    Lane 3: NCCIT (human pluripotent embryonic carcinoma epithelial cell) whole cell lysate at 50 µg

    Lane 4: SW-13 (human adrenal gland cortex epithelial cell) whole cell lysate at 50 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 200 kDa, 124 kDa

    Exposure time: 81s

  • Western blot - Anti-SMARCA2 / BRM antibody [EPR28611-92] (ab323179), expandable thumbnail

    Western blot - Anti-SMARCA2 / BRM antibody [EPR28611-92] (ab323179)

    SMARCA2 / BRM Western blot staining using rabbit Anti-SMARCA2 / BRM antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In lane 1, the lysate was stored at -80℃ prior to Western Blotting. The bands beneath the target band (200 kDa) are likely to be degradation products. In lane 2, To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-SMARCA2 / BRM antibody [EPR28611-92] (ab323179) at 1/1000 dilution

    All lanes: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 200 kDa, 124 kDa

    Exposure time: 180s

  • Western blot - Anti-SMARCA2 / BRM antibody [EPR28611-92] (ab323179), expandable thumbnail

    Western blot - Anti-SMARCA2 / BRM antibody [EPR28611-92] (ab323179)

    SMARCA2 / BRM Western blot staining using rabbit Anti-SMARCA2 / BRM antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with human BRG1.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-SMARCA2 / BRM antibody [EPR28611-92] (ab323179) at 1/1000 dilution

    Lane 1: His-tagged human SMARCA2/BRM fragment at 10 ng

    Lane 2: His-tagged human BRG1 fragment at 10 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 28,36 kDa, 20-36 kDa

    Exposure time: 8s

  • ChIC/CUT&RUN sequencing - Anti-SMARCA2 / BRM antibody [EPR28611-92] (ab323179), expandable thumbnail

    ChIC/CUT&RUN sequencing - Anti-SMARCA2 / BRM antibody [EPR28611-92] (ab323179)

    ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 105 HeLa (human cervix adenocarcinoma epithelial cell) cells and 5 µg of ab323179[EPR28611-92]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.

    The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

  • ChIC/CUT&RUN sequencing - Anti-SMARCA2 / BRM antibody [EPR28611-92] (ab323179), expandable thumbnail

    ChIC/CUT&RUN sequencing - Anti-SMARCA2 / BRM antibody [EPR28611-92] (ab323179)

    ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 105 HeLa (human cervix adenocarcinoma epithelial cell) cells and 5 µg of ab323179[EPR28611-92]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.

    The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

  • ChIC/CUT&RUN sequencing - Anti-SMARCA2 / BRM antibody [EPR28611-92] (ab323179), expandable thumbnail

    ChIC/CUT&RUN sequencing - Anti-SMARCA2 / BRM antibody [EPR28611-92] (ab323179)

    ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 105 HeLa (human cervix adenocarcinoma epithelial cell) cells and 5 µg of ab323179[EPR28611-92]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.

    The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

  • Immunoprecipitation - Anti-SMARCA2 / BRM antibody [EPR28611-92] (ab323179), expandable thumbnail

    Immunoprecipitation - Anti-SMARCA2 / BRM antibody [EPR28611-92] (ab323179)

    SMARCA2 / BRM was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab323179 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab323179 at 1000 dilution.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST

    To minimize protein degradation, cells were lysed immediately after harvest and then applied for Immunoprecipitation as soon as possible.

    All lanes: Immunoprecipitation - Anti-SMARCA2 / BRM antibody [EPR28611-92] (ab323179) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 10 µg

    Lane 2: ab323179 at 1/30 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 10 µg

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab323179 in HeLa whole cell lysate at 10 µg

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Observed band size: 200 kDa

    Exposure time: 15s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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