AB3851

Anti-SMARCA6 antibody

Name: Anti-SMARCA6 antibody (ab3851) | Abcam
Brand: Abcam Limited
SKU: AB3851
Price: 925 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(5 Publications)

Rabbit Polyclonal SMARCA6 antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human HELLS.

View Alternative Names

PASG, SMARCA6, Nbla10143, HELLS, Lymphoid-specific helicase, LSH, Proliferation-associated SNF2-like protein, SWI/SNF2-related matrix-associated actin-dependent regulator of chromatin subfamily A member 6

2 Images

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Western blot - Anti-SMARCA6 antibody (AB3851)

All lanes:

Western blot - Anti-SMARCA6 antibody (ab3851) at 1 µg/mL

All lanes:

Human thymus tissue lysate - total protein (<a href='/en-us/products/unavailable/human-thymus-tissue-lysate-total-protein-ab30146'>ab30146</a>) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 97 kDa

Observed band size: 102 kDa,120 kDa,47 kDa,97 kDa

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Exposure time: 30s

Supplier Data

Western blot - Anti-SMARCA6 antibody (AB3851)

All lanes:

Western blot - Anti-SMARCA6 antibody (ab3851) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

K562 whole cell lysate at 50 µg

Predicted band size: 97 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human HELLS. The exact immunogen used to generate this antibody is proprietary information.

Q9NRZ9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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Product details

DNA helicases (Hel) play a role in a number of processes involving DNA strand separation, including replication, repair, recombination, and transcription. Jarvis et al. (1996) identified a mouse gene encoding a novel putative helicase. They designated the gene LSH, for lymphoid-specific Hel, because Northern blot analysis revealed that it was expressed specifically in mouse early thymocytes. Geiman et al. (1998) determined that the mouse LSH, or HELLS (helicase lymphoid-specific), gene contains at least 18 exons and spans more than 26.6 kb. Using Northern blot analysis, these authors found that human HELLS is expressed in testis and thymus, two tissues that are highly active in DNA recombination. Geiman and Muegge (2000) demonstrated that LSH is not obligatory for normal lymphoid development but is essential for normal proliferation of peripheral T lymphocytes.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMARCA6 also known as SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 6 is a protein with a molecular weight of approximately 170 kDa. It functions as an ATPase and is an important component of chromatin remodeling complexes. SMARCA6 plays a role in modifying chromatin structure through ATP hydrolysis which influences gene expression. This protein is expressed in various tissues and its expression can be detected across different cell types including those involved in blood functions.

Biological function summary

SMARCA6 influences gene expression by modifying the chromatin structure which allows transcription factors to access DNA. It is a part of the SWI/SNF chromatin remodeling complex critical for regulating a wide range of genes involved in development and cell cycle. Through the remodeling of chromatin SMARCA6 controls access to specific genomic regions and modulates transcriptional activation or repression therefore influencing cellular differentiation and proliferation.

Pathways

SMARCA6 participates in pathways involved in chromatin organization and gene regulation. It engages in the Wnt signaling pathway interacting with proteins such as BAF155 and BAF170 to modulate transcriptional outcomes. SMARCA6's activity in these pathways is essential for cellular processes impacting how cells respond to growth signals and maintain homeostasis.

Aberrations in SMARCA6 function or expression can lead to complications such as cancer and neurodevelopmental disorders. It has been linked to leukemia where chromatin remodeling anomalies disrupt normal gene expression patterns. In such contexts SMARCA6 interacts with proteins like BAF57 affecting the regulation of oncogenes and tumor suppressors. Altered SMARCA6 activity in these diseases highlights its role in maintaining genomic stability and proper cell function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

ATP-dependent chromatin remodeler that regulates chromatin accessibility, DNA methylation, and histone modifications. It facilitates de novo DNA methylation at repetitive sequences and promotes transcriptional silencing via recruitment of DNA methyltransferases (DNMTs) and histone deacetylases (HDACs), contributing to heterochromatin formation and repression of transposable elements (PubMed : 30307408). Also involved in DNA repair by recruiting DNA damage response mediators to double-strand breaks in heterochromatin, promoting homologous recombination via RBBP8/CtIP-dependent end resection (PubMed : 22946062, PubMed : 31802118). During meiosis, it is recruited by PRDM9 to recombination hotspots, aiding chromatin opening (PubMed : 32001511). Through these diverse roles, is crucial for processes such as development, differentiation, and genomic stability (PubMed : 22946062, PubMed : 31802118). Involved in regulation of the expansion or survival of lymphoid cells (By similarity).

See full target information HELLS

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

PeerJ 11:e16451 PubMed38047020

2023

Lymphoid-specific helicase inhibits cervical cancer cells ferroptosis by promoting Nrf2 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Tie,Fenfen Ge

Cell death & disease 14:402 PubMed37414755

2023

USP11-mediated LSH deubiquitination inhibits ferroptosis in colorectal cancer through epigenetic activation of CYP24A1.

Applications

Unspecified application

Species

Unspecified reactive species

Junyi Duan,Daoyuan Huang,Cheng Liu,Yangbo Lv,Lei Zhang,Fen Chang,Xiangyu Zeng,Li Li,Weiping Wang,Genze Shao

Bioengineered 13:8311-8322 PubMed35311456

2022

Hsa_circ_0072008 regulates cell proliferation, migration, and invasion in cervical squamous cell carcinoma via miR-1305/helicase, lymphoid specific (HELLS) axis.

Applications

Unspecified application

Species

Unspecified reactive species

Chunhua He,Leng Liu

Proceedings of the National Academy of Sciences of 115:E876-E885 PubMed29339483

2018

HELLS and CDCA7 comprise a bipartite nucleosome remodeling complex defective in ICF syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher Jenness,Simona Giunta,Manuel M Müller,Hiroshi Kimura,Tom W Muir,Hironori Funabiki

Epigenomes 1: PubMed31867127

2017

Helicase Lymphoid-Specific Enzyme Contributes to the Maintenance of Methylation of SST1 Pericentromeric Repeats That Are Frequently Demethylated in Colon Cancer and Associate with Genomic Damage.

Applications

Unspecified application

Species

Unspecified reactive species

Johanna K Samuelsson,Gabrijela Dumbovic,Cristian Polo,Cristina Moreta,Andreu Alibés,Tatiana Ruiz-Larroya,Pepita Giménez-Bonafé,Sergio Alonso,Sonia-V Forcales,Perucho Manuel

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SMARCA6 antibody

Western blot - Anti-SMARCA6 antibody (AB3851)

Western blot - Anti-SMARCA6 antibody (AB3851)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise