Rabbit Polyclonal SmarcAL1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SMARCAL1 aa 100-500.
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Publications
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- Molecular cell 81:2989-3006.e92021EXO5-DNA structure and BLM interactions direct DNA resection critical for ATR-dependent replication restart.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShashank Hambarde,Chi-Lin Tsai,Raj K Pandita,Albino Bacolla,Anirban Maitra,Vijay Charaka,Clayton R Hunt,Rakesh Kumar,Oliver Limbo,Remy Le Meur,Walter J Chazin,Susan E Tsutakawa,Paul Russell,Katharina Schlacher,Tej K Pandita,John A TainerPubMed 34197737
- Disease models & mechanisms 12:2019Inducible SMARCAL1 knockdown in iPSC reveals a link between replication stress and altered expression of master differentiation genes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGiusj Monia Pugliese et. al.PubMed 31515241
- Nucleic acids research 43:6359-722015Smarcal1 promotes double-strand-break repair by nonhomologous end-joining.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SMARCAL1 aa 100-500. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NZC9
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
ATP-dependent annealing helicase that binds selectively to fork DNA relative to ssDNA or dsDNA and catalyzes the rewinding of the stably unwound DNA. Rewinds single-stranded DNA bubbles that are stably bound by replication protein A (RPA). Acts throughout the genome to reanneal stably unwound DNA, performing the opposite reaction of many enzymes, such as helicases and polymerases, that unwind DNA. May play an important role in DNA damage response by acting at stalled replication forks.
Alternative names
HARP, SMARCAL1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1, HepA-related protein, Sucrose nonfermenting protein 2-like 1, hHARP
Recommended products
Rabbit Polyclonal SmarcAL1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SMARCAL1 aa 100-500.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SMARCAL1 aa 100-500. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NZC9
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SmarcAL1 also known as SWI/SNF-related ATPase family member A1 is a DNA-dependent ATPase that unwinds DNA during replication and repair. It also plays a role in chromatin remodeling. The molecular mass of SmarcAL1 is approximately 105 kDa. SmarcAL1 expression is particularly noticeable in tissues with high replicative demands like testis and ovary among others.
- Biological function summary
SmarcAL1 facilitates proper replication fork stability and functioning by associating with other proteins and complexes. It is a part of the SWI/SNF chromatin remodeling complex which alters the structure of chromatin to regulate access to DNA. This protein works with other chromatin remodeling factors to ensure the DNA replication and repair processes run smoothly.
- Pathways
SmarcAL1 is a significant player in DNA damage response and repair pathways. It is actively involved in the homologous recombination repair pathway. Associated proteins within this pathway include BRCA1 and RAD51 which are essential for repairing double-strand breaks in DNA. Together these proteins promote genomic stability by correcting DNA damage and supporting normal cell cycle progression.
- Associated diseases and disorders
Mutations or dysfunctions in SmarcAL1 have connections to Schimke immuno-osseous dysplasia (SIOD) and various cancer types. These associations arise because SmarcAL1 influences genome stability and cell cycle progression. The connection of SmarcAL1 with BRCA1 highlights its relevance in breast cancer where malfunction in DNA repair mechanisms contribute to cancer development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-SmarcAL1 antibody (ab154226)
5% SDS PAGE
All lanes: Western blot - Anti-SmarcAL1 antibody (ab154226) at 1/500 dilution
All lanes: A549 whole cell lysate at 30 µg
Predicted band size: 106 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SmarcAL1 antibody (ab154226)
Immunofluorescence analysis of paraformaldehyde-fixed A549 cells, labeling SmarcAL1 with ab154226 at a 1/500 dilution (top image), and costained with Hoescht 33342 (bottom image).
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