AB154226

Anti-SmarcAL1 antibody

Name: Anti-SmarcAL1 antibody (ab154226) | Abcam
Brand: Abcam Limited
SKU: AB154226
Price: 495 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(4 Publications)

Rabbit Polyclonal SmarcAL1 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SMARCAL1 aa 100-500.

View Alternative Names

HARP, SMARCAL1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1, HepA-related protein, Sucrose nonfermenting protein 2-like 1, hHARP

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-SmarcAL1 antibody (AB154226)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SmarcAL1 antibody (AB154226)

Immunofluorescence analysis of paraformaldehyde-fixed A549 cells, labeling SmarcAL1 with ab154226 at a 1/500 dilution (top image), and costained with Hoescht 33342 (bottom image).

Supplier Data

Immunoprecipitation - Anti-SmarcAL1 antibody (AB154226)

SmarcAL1 antibody immunoprecipitates SMARCAL1 protein in IP experiments. Samples were separated by 5% SDS PAGE. The immunoprecipitated SMARCAL1 protein was detected by SmarcAL1 antibody diluted at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-SmarcAL1 antibody (ab154226) at 4 µg

Lane 1:

A549 whole cell extract with preimmune Rabbit IgG control

Lane 2:

Immunoprecipitated A549 whole cell extract using ab154226

Secondary

All lanes:

EasyBlot anti-Rabbit IgG

Predicted band size: 106 kDa

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Supplier Data

Western blot - Anti-SmarcAL1 antibody (AB154226)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-SmarcAL1 antibody (ab154226) at 1/1000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Lane 3:

HeLa whole cell lysates at 30 µg

Lane 4:

HepG2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 106 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SMARCAL1 aa 100-500. The exact immunogen used to generate this antibody is proprietary information.

Q9NZC9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SmarcAL1 also known as SWI/SNF-related ATPase family member A1 is a DNA-dependent ATPase that unwinds DNA during replication and repair. It also plays a role in chromatin remodeling. The molecular mass of SmarcAL1 is approximately 105 kDa. SmarcAL1 expression is particularly noticeable in tissues with high replicative demands like testis and ovary among others.

Biological function summary

SmarcAL1 facilitates proper replication fork stability and functioning by associating with other proteins and complexes. It is a part of the SWI/SNF chromatin remodeling complex which alters the structure of chromatin to regulate access to DNA. This protein works with other chromatin remodeling factors to ensure the DNA replication and repair processes run smoothly.

Pathways

SmarcAL1 is a significant player in DNA damage response and repair pathways. It is actively involved in the homologous recombination repair pathway. Associated proteins within this pathway include BRCA1 and RAD51 which are essential for repairing double-strand breaks in DNA. Together these proteins promote genomic stability by correcting DNA damage and supporting normal cell cycle progression.

Mutations or dysfunctions in SmarcAL1 have connections to Schimke immuno-osseous dysplasia (SIOD) and various cancer types. These associations arise because SmarcAL1 influences genome stability and cell cycle progression. The connection of SmarcAL1 with BRCA1 highlights its relevance in breast cancer where malfunction in DNA repair mechanisms contribute to cancer development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

ATP-dependent annealing helicase that binds selectively to fork DNA relative to ssDNA or dsDNA and catalyzes the rewinding of the stably unwound DNA. Rewinds single-stranded DNA bubbles that are stably bound by replication protein A (RPA). Acts throughout the genome to reanneal stably unwound DNA, performing the opposite reaction of many enzymes, such as helicases and polymerases, that unwind DNA. May play an important role in DNA damage response by acting at stalled replication forks.

See full target information SMARCAL1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell 187:861-881.e32 PubMed38301646

2024

SMARCAL1 is a dual regulator of innate immune signaling and PD-L1 expression that promotes tumor immune evasion.

Applications

Unspecified application

Species

Unspecified reactive species

Giuseppe Leuzzi,Alessandro Vasciaveo,Angelo Taglialatela,Xiao Chen,Tessa M Firestone,Allison R Hickman,Wendy Mao,Tanay Thakar,Alina Vaitsiankova,Jen-Wei Huang,Raquel Cuella-Martin,Samuel B Hayward,Jordan S Kesner,Ali Ghasemzadeh,Tarun S Nambiar,Patricia Ho,Alexander Rialdi,Maxime Hebrard,Yinglu Li,Jinmei Gao,Saarang Gopinath,Oluwatobi A Adeleke,Bryan J Venters,Charles G Drake,Richard Baer,Benjamin Izar,Ernesto Guccione,Michael-Christopher Keogh,Raphael Guerois,Lu Sun,Chao Lu,Andrea Califano,Alberto Ciccia

Molecular cell 81:2989-3006.e9 PubMed34197737

2021

EXO5-DNA structure and BLM interactions direct DNA resection critical for ATR-dependent replication restart.

Applications

Unspecified application

Species

Unspecified reactive species

Shashank Hambarde,Chi-Lin Tsai,Raj K Pandita,Albino Bacolla,Anirban Maitra,Vijay Charaka,Clayton R Hunt,Rakesh Kumar,Oliver Limbo,Remy Le Meur,Walter J Chazin,Susan E Tsutakawa,Paul Russell,Katharina Schlacher,Tej K Pandita,John A Tainer

Disease models & mechanisms 12: PubMed31515241

2019

Inducible SMARCAL1 knockdown in iPSC reveals a link between replication stress and altered expression of master differentiation genes.

Applications

Unspecified application

Species

Unspecified reactive species

Giusj Monia Pugliese,Federico Salaris,Valentina Palermo,Veronica Marabitti,Nicolò Morina,Alessandro Rosa,Annapaola Franchitto,Pietro Pichierri

Nucleic acids research 43:6359-72 PubMed26089390

2015

Smarcal1 promotes double-strand-break repair by nonhomologous end-joining.

Applications

Unspecified application

Species

Human

Islam Shamima Keka, Mohiuddin,Yuko Maede,Md Maminur Rahman,Tetsushi Sakuma,Masamitsu Honma,Takashi Yamamoto,Shunichi Takeda,Hiroyuki Sasanuma

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SmarcAL1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-SmarcAL1 antibody (AB154226)

Immunoprecipitation - Anti-SmarcAL1 antibody (AB154226)

Western blot - Anti-SmarcAL1 antibody (AB154226)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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