JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB327207

Anti-SMARCC1 Antibody

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Polyclonal SMARCC1/BAF155 antibody. Suitable for WB, IHC-P, ICC/IF, ChIP and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SMARCC1.

View Alternative Names

BAF155, SMARCC1, SWI/SNF complex subunit SMARCC1, BRG1-associated factor 155, SWI/SNF complex 155 kDa subunit, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCC1 Antibody (AB327207)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCC1 Antibody (AB327207)

Immunohistochemical staining of human placenta using ab327207 at 1 : 500–1 : 1000 dilution, showing strong nuclear positivity in trophoblastic cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCC1 Antibody (AB327207)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCC1 Antibody (AB327207)

Immunohistochemical staining of human tonsil using ab327207 at 1 : 500–1 : 1000 dilution, showing strong nuclear positivity in germinal center cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCC1 Antibody (AB327207)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCC1 Antibody (AB327207)

Immunohistochemical staining of human placenta, skeletal muscle, testis and tonsil using ab327207 at 1 : 500–1 : 1000 dilution (A) shows similar protein distribution across tissues to an independent Anti-SMARCC1 antibody (B).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCC1 Antibody (AB327207)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCC1 Antibody (AB327207)

Immunohistochemistry analysis in human testis and skeletal muscle tissues using ab327207 at 1 : 500–1 : 1000 dilution. Corresponding SMARCC1 RNA-seq data are presented for the same tissues.

Immunocytochemistry/ Immunofluorescence - Anti-SMARCC1 Antibody (AB327207)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SMARCC1 Antibody (AB327207)

Immunofluorescent staining of human cell line U-2 OS using ab327207 at 0.25–2 µg/ml, showing localization to nucleoplasm.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCC1 Antibody (AB327207)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCC1 Antibody (AB327207)

Immunohistochemical staining of human testis using ab327207 at 1 : 500–1 : 1000 dilution, showing strong nuclear positivity in cells in seminiferous ducts.

ChIP - Anti-SMARCC1 Antibody (AB327207)
  • ChIP

Supplier Data

ChIP - Anti-SMARCC1 Antibody (AB327207)

ChIP-Exo-Seq composite graph for ab327207 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Western blot - Anti-SMARCC1 Antibody (AB327207)
  • WB

Supplier Data

Western blot - Anti-SMARCC1 Antibody (AB327207)

Western blot analysis in human cell line NTERA-2 using ab327207 at 0.04–0.4 µg/ml.

All lanes:

Western blot - Anti-SMARCC1 Antibody (ab327207)

All lanes:

Human cell line NTERA-2

Predicted band size: 122.87 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCC1 Antibody (AB327207)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCC1 Antibody (AB327207)

Immunohistochemical staining of human skeletal muscle using ab327207 at 1 : 500–1 : 1000 dilution, showing no positivity in myocytes as expected.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, ChIP, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SMARCC1.

Q92922

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "1-10 µg", "ChIP-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. May stimulate the ATPase activity of the catalytic subunit of the complex (PubMed : 10078207, PubMed : 29374058). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity).
See full target information SMARCC1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com