Anti-SMARCC1/BAF155 antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SMARCC1/BAF155 antibody (AB126180)

Immunofluorescence analysis of 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell labeling SMARCC1/BAF155 protein (green) with ab126180 at 1/500 dilution. Red : phalloidin, a cytoskeleton marker, at 1/200 dilution. Scale bar= 10 μm.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SMARCC1/BAF155 antibody (AB126180)

Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell labeling SMARCC1/BAF155 protein (green) with ab126180 at 1/500 dilution. Red : Alpha-tubulin filaments, at 1/2000 dilution.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCC1/BAF155 antibody (AB126180)

Immunohistochemical analysis of paraffin-embedded human BT483 xenograft staining SMARCC1/BAF155 protein using ab126180 at 1/500 dilution.

Antigen Retrieval : EDTA based buffer, pH 8.0, 15min.

• ChIP

Supplier Data

ChIP - Anti-SMARCC1/BAF155 antibody (AB126180)

Cross-linked ChIP was performed with MCF7 (human breast adenocarcinoma cell line) chromatin extract treated with B-estradiol (10 nM for 45 min) and 5 μg of either control rabbit IgG or ab126180. The precipitated DNA was detected by PCR with primer set targeting to ESR1 or Sat2.

• IP

Supplier Data

Immunoprecipitation - Anti-SMARCC1/BAF155 antibody (AB126180)

ab126180 immunoprecipitates SMARCC1 protein using HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate.

IP Sample :

1- HEK-293T whole cell lysate, 30 μg;

2- Control with 2.5 μg of pre-immune rabbit IgG;

3- Immunoprecipitation of SMARCC1 by 2.5 μg of ab126180.

5% SDS-PAGE

The immunoprecipitated SMARCC1 protein was detected byab126180 at 1/1000 dilution, followed by anti-rabbit IgG (HRP) antibody.

All lanes:

Immunoprecipitation - Anti-SMARCC1/BAF155 antibody (ab126180)

Predicted band size: 123 kDa

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• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCC1/BAF155 antibody (AB126180)

Immunohistochemical analysis of paraffin-embedded rat hind brain staining SMARCC1/BAF155 protein at nucleus using ab126180 at 1/500 dilution.

Antigen Retrieval : EDTA based buffer, pH 8.0, 15min.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCC1/BAF155 antibody (AB126180)

Immunohistochemical analysis of paraffin-embedded mouse prostate staining SMARCC1/BAF155 protein at nucleus using ab126180 at 1/500 dilution.

Antigen Retrieval : EDTA based buffer, pH 8.0, 15min.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCC1/BAF155 antibody (AB126180)

Immunohistochemical analysis of paraffin-embedded C2C12 (mouse myoblast cell line) xenograft staining SMARCC1/BAF155 protein using ab126180 at 1/500 dilution.

Antigen Retrieval : EDTA based buffer, pH 8.0, 15min.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCC1/BAF155 antibody (AB126180)

Immunohistochemical analysis of paraffin-embedded mouse duodenum staining SMARCC1/BAF155 protein at nucleus using ab126180 at 1/500 dilution.

Antigen Retrieval : EDTA based buffer, pH 8.0, 15min.

• WB

Lab

Western blot - Anti-SMARCC1/BAF155 antibody (AB126180)

5% SDS-PAGE

All lanes:

Western blot - Anti-SMARCC1/BAF155 antibody (ab126180) at 1/5000 dilution

Lane 1:

Wild-Type HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Lane 2:

SMARCC1/BAF155 Knockout HeLa cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 123 kDa

false

• WB

Supplier Data

Western blot - Anti-SMARCC1/BAF155 antibody (AB126180)

5% SDS-PAGE

All lanes:

Western blot - Anti-SMARCC1/BAF155 antibody (ab126180) at 1/2000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Lane 4:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 123 kDa

false

• WB

Supplier Data

Western blot - Anti-SMARCC1/BAF155 antibody (AB126180)

5% SDS-PAGE

All lanes:

Western blot - Anti-SMARCC1/BAF155 antibody (ab126180) at 1/1000 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 30 µg

Lane 2:

C8-D30 (mouse astrocyte type III cerebellum cell line) whole cell lysate at 30 µg

Lane 3:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

Lane 4:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 30 µg

Lane 5:

C2C12 (mouse myoblast cell line) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 123 kDa

false

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCC1/BAF155 antibody (AB126180)

Immunohistochemical analysis of paraffin-embedded RT2 xenograft staining SMARCC1/BAF155 protein using ab126180 at 1/500 dilution.

Antigen Retrieval : EDTA based buffer, pH 8.0, 15min.