AB72503

Anti-SMARCC1/BAF155 antibody

Name: Anti-SMARCC1/BAF155 antibody (ab72503) | Abcam
Brand: Abcam Limited
SKU: AB72503
Price: 535 USD
Availability: InStock

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Polyclonal SMARCC1/BAF155 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SMARCC1.

View Alternative Names

BAF155, SMARCC1, SWI/SNF complex subunit SMARCC1, BRG1-associated factor 155, SWI/SNF complex 155 kDa subunit, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-SMARCC1/BAF155 antibody (AB72503)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SMARCC1/BAF155 antibody (AB72503)

ICC/IF image of ab72503 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72503, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Unknown

Immunoprecipitation - Anti-SMARCC1/BAF155 antibody (AB72503)

Detection of SMARCC1/BAF155 by Western Blot of Immunprecipitate.
ab72503 at 1μg/ml staining SMARCC1/BAF155 in HeLa whole cell lysate immunoprecipitated using ab72503 at 3μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 1 second.

All lanes:

Immunoprecipitation - Anti-SMARCC1/BAF155 antibody (ab72503)

Predicted band size: 123 kDa

false

Unknown

Western blot - Anti-SMARCC1/BAF155 antibody (AB72503)

All lanes:

Western blot - Anti-SMARCC1/BAF155 antibody (ab72503) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 123 kDa

Observed band size: 160 kDa

false

Exposure time: 1s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP, ICC/IF

applications

Immunogen

Synthetic Peptide within Human SMARCC1. The exact immunogen used to generate this antibody is proprietary information.

Q92922

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab72503 was affinity purified using an epitope specific to SMARCC1/BAF155 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMARCC1 also known as BAF155 is a component of the SWI/SNF chromatin remodeling complex with a molecular weight of approximately 155 kDa. It plays a role in modifying the structure of chromatin which affects the accessibility of DNA to transcription factors and other proteins. SMARCC1 expresses widely in various tissues particularly where regulation of gene transcription is essential. This protein acts as a scaffold facilitating the assembly of the SWI/SNF complex which is vital for the regulation of transcriptional processes.

Biological function summary

SMARCC1 participates in remodeling chromatin architecture to facilitate or repress gene transcription. It forms an integral part of the SWI/SNF complex which is critical for maintaining proper chromatin structure and function. This complex influences gene expression during developmental processes cell cycle regulation and DNA repair. SMARCC1 interacts with other core components of the SWI/SNF complex such as BAF47 and BRG1 ensuring correct and responsive chromatin modification.

Pathways

SMARCC1 participates in the ATP-dependent chromatin remodeling pathway an important player in gene expression control. This remodeling is connected to the cell cycle pathway as it regulates genes required for cell proliferation. Within these pathways SMARCC1 closely interacts with transcription factors such as c-Myc and other chromatin regulators coordinating the expression of target genes necessary for cell growth and differentiation.

SMARCC1 is linked to cancer and neurodevelopmental disorders. Alterations in SMARCC1 function or expression can lead to dysregulated gene expression contributing to tumorigenesis by affecting pathways involved in cell growth and apoptosis. For instance its association with BRG1 influences pathways implicated in various cancers including solid tumors. Additionally SMARCC1 dysfunction connects to certain neurodevelopmental disorders through the modification of genes critical for brain development and function.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. May stimulate the ATPase activity of the catalytic subunit of the complex (PubMed : 10078207, PubMed : 29374058). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity).

See full target information SMARCC1

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Oncogene 40:3593-3609 PubMed33931740

2021

Multiple interactions of the oncoprotein transcription factor MYC with the SWI/SNF chromatin remodeler.

Applications

Unspecified application

Species

Unspecified reactive species

Chase M Woodley,Alexander S Romer,Jing Wang,Alissa D Guarnaccia,David L Elion,Jack N Maxwell,Kiana Guerrazzi,Tyler S McCann,Tessa M Popay,Brittany K Matlock,David K Flaherty,Shelly L Lorey,Qi Liu,William P Tansey,April M Weissmiller

PLoS genetics 8:e1002571 PubMed22438823

2012

Transcriptional control in cardiac progenitors: Tbx1 interacts with the BAF chromatin remodeling complex and regulates Wnt5a.

Applications

Species

Mouse

Li Chen,Filomena Gabriella Fulcoli,Rosa Ferrentino,Stefania Martucciello,Elizabeth A Illingworth,Antonio Baldini

View all publications

style

left-column

style

left-column

style

right-column