Anti-SMARCC1/BAF155 antibody [EPR12389] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SMARCC1/BAF155 antibody [EPR12389] - BSA and Azide free (AB249725)

This data was developed using ab172636, the same antibody clone in a different buffer formulation.

Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling SMARCC1/BAF155 with ab172636 at a 1/100 dilution.

• WB

Lab

Western blot - Anti-SMARCC1/BAF155 antibody [EPR12389] - BSA and Azide free (AB249725)

This data was developed using ab172636, the same antibody clone in a different buffer formulation.

Lanes 1 - 4 : Merged signal (red and green). Green - ab172636 observed at 123 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab172636 was shown to specifically react with SMARCC1 in wild-type HEK-293 cells as signal was lost in SMARCC1 knockout cells. Wild-type and SMARCC1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab172636 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-SMARCC1/BAF155 antibody [EPR12389] (<a href='/en-us/products/primary-antibodies/smarcc1-baf155-antibody-epr12389-ab172636'>ab172636</a>) at 1/5000 dilution

Lane 1:

Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

SMARCC1 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

Western blot - Human SMARCC1 (BAF155) knockout HEK-293 cell line (<a href='/en-us/products/cell-lines/human-smarcc1-baf155-knockout-hek-293-cell-line-ab261854'>ab261854</a>)

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 4:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Predicted band size: 123 kDa

Observed band size: 123 kDa

false

• WB

Supplier Data

Western blot - Anti-SMARCC1/BAF155 antibody [EPR12389] - BSA and Azide free (AB249725)

This data was developed using ab172636, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SMARCC1/BAF155 antibody [EPR12389] (<a href='/en-us/products/primary-antibodies/smarcc1-baf155-antibody-epr12389-ab172636'>ab172636</a>) at 1/1000 dilution

Lane 1:

NTera2/D1 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Predicted band size: 123 kDa

false