Anti-SMARCC1/BAF155 antibody [EPR25109-77] KO Tested (ab305037)

Knockout Tested Rabbit Recombinant Monoclonal SMARCC1/BAF155 antibody. Suitable for WB, IHC-P, ICC/IF, IP and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

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Images

Immunoprecipitation - Anti-SMARCC1/BAF155 antibody [EPR25109-77] (AB305037), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	ICC/IF	Flow Cyt (Intra)	IP	ChIP
Human	Tested	Tested	Tested	Not recommended	Tested	Not recommended
Mouse	Tested	Tested	Tested	Not recommended	Tested	Not recommended
Rat	Tested	Tested	Expected	Not recommended	Expected	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/100	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/100	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes -
Species Human	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/30	Notes -
Species Human	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Human, Rat	Dilution info -	Notes -

Target data

See full target information SMARCC1

Function

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. May stimulate the ATPase activity of the catalytic subunit of the complex (PubMed:10078207, PubMed:29374058). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity).

Alternative names

BAF155, SMARCC1, SWI/SNF complex subunit SMARCC1, BRG1-associated factor 155, SWI/SNF complex 155 kDa subunit, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal SMARCC1/BAF155 antibody. Suitable for WB, IHC-P, ICC/IF, IP and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR25109-77
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

SMARCC1 also known as BAF155 is a component of the SWI/SNF chromatin remodeling complex with a molecular weight of approximately 155 kDa. It plays a role in modifying the structure of chromatin which affects the accessibility of DNA to transcription factors and other proteins. SMARCC1 expresses widely in various tissues particularly where regulation of gene transcription is essential. This protein acts as a scaffold facilitating the assembly of the SWI/SNF complex which is vital for the regulation of transcriptional processes.

Biological function summary

SMARCC1 participates in remodeling chromatin architecture to facilitate or repress gene transcription. It forms an integral part of the SWI/SNF complex which is critical for maintaining proper chromatin structure and function. This complex influences gene expression during developmental processes cell cycle regulation and DNA repair. SMARCC1 interacts with other core components of the SWI/SNF complex such as BAF47 and BRG1 ensuring correct and responsive chromatin modification.

Pathways

SMARCC1 participates in the ATP-dependent chromatin remodeling pathway an important player in gene expression control. This remodeling is connected to the cell cycle pathway as it regulates genes required for cell proliferation. Within these pathways SMARCC1 closely interacts with transcription factors such as c-Myc and other chromatin regulators coordinating the expression of target genes necessary for cell growth and differentiation.

Associated diseases and disorders

SMARCC1 is linked to cancer and neurodevelopmental disorders. Alterations in SMARCC1 function or expression can lead to dysregulated gene expression contributing to tumorigenesis by affecting pathways involved in cell growth and apoptosis. For instance its association with BRG1 influences pathways implicated in various cancers including solid tumors. Additionally SMARCC1 dysfunction connects to certain neurodevelopmental disorders through the modification of genes critical for brain development and function.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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15 product images

Immunoprecipitation - Anti-SMARCC1/BAF155 antibody [EPR25109-77] (ab305037)
SMARCC1/BAF155 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 10 ug with ab305037 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab305037 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 10 ug

Lane 2: ab305037 IP in HeLa whole cell lysate

Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab305037 in HeLa whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 76 seconds

The band beneath the target band (150 kDa) is likely to be degraded target fragments.
All lanes: Immunoprecipitation - Anti-SMARCC1/BAF155 antibody [EPR25109-77] (ab305037) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 10 ug
Lane 2: ab305037 IP in HeLa whole cell lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Predicted band size: 150 kDa
Observed band size: 150 kDa
Exposure time: 76s
Immunoprecipitation - Anti-SMARCC1/BAF155 antibody [EPR25109-77] (ab305037)
SMARCC1/BAF155 was immunoprecipitated from 0.35 mg ES-D3 (mouse embryonic pluripotent stem Cell), whole cell lysate 10 ug with ab305037 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab305037 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: ES-D3 (mouse embryonic pluripotent stem Cell), whole cell lysate 10 ug

Lane 2: ab305037 IP in ES-D3 whole cell lysate

Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab305037 in ES-D3 whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 15 seconds

The band beneath the target band (150 kDa) is likely to be degraded target fragments.
All lanes: Immunoprecipitation - Anti-SMARCC1/BAF155 antibody [EPR25109-77] (ab305037) at 1/1000 dilution
Lane 1: ES-D3 (mouse embryonic pluripotent stem Cell), whole cell lysate 10 ug
Lane 2: ab305037 IP in ES-D3 whole cell lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Predicted band size: 150 kDa
Observed band size: 150 kDa
Exposure time: 15s
Immunocytochemistry/ Immunofluorescence - Anti-SMARCC1/BAF155 antibody [EPR25109-77] (ab305037)
Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized ES-D3 [D3] (mouse blastocyst-derived embryonic stem cell) cells labelling SMARCC1/BAF155 with ab305037 at 1/100 (4.75 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing nuclear staining in ES-D3 [D3] cell line.The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8) is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
Western blot - Anti-SMARCC1/BAF155 antibody [EPR25109-77] (ab305037)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
In Western blot, ab305037 was shown to bind specifically to SMARCC1/BAF155. A band was observed at 150 kDa in wild-type HeLa cell lysate whereas no signal observed at this size in SMARCC1/BAF155 knockout cell line.

The band beneath the target band (150 kDa) is likely to be degraded target fragments.

ES-D3 cell Lysate was freshly made and used immediately to minimize protein degradation.
Exposure time: Lanes 1-2: 26 seconds
Lane 3: 6 seconds
All lanes: Western blot - Anti-SMARCC1/BAF155 antibody [EPR25109-77] (ab305037) at 1/1000 dilution
Lane 1: Wild-type HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2: SWI/SNF complex subunit SMARCC1 knockout HeLa, whole cell lysate at 20 µg
Lane 3: ES-D3 (mouse embryonic pluripotent stem Cell), whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 123 kDa
Observed band size: 150 kDa
Western blot - Anti-SMARCC1/BAF155 antibody [EPR25109-77] (ab305037)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Lysates were freshly made and used immediately to minimize protein degradation.

The band beneath the target band (150 kDa) is likely to be degraded target fragments.

Exposure time: 26 seconds
All lanes: Western blot - Anti-SMARCC1/BAF155 antibody [EPR25109-77] (ab305037) at 1/1000 dilution
All lanes: Mouse spleen tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 123 kDa
Observed band size: 150 kDa
Exposure time: 26s
Western blot - Anti-SMARCC1/BAF155 antibody [EPR25109-77] (ab305037)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody does not cross-react with mouse SMARCC2/BAF170.
Exposure time: 37 seconds
All lanes: Western blot - Anti-SMARCC1/BAF155 antibody [EPR25109-77] (ab305037) at 1/1000 dilution
Lane 1: His-tagged mouse SMARCC1/BAF155 recombinant protein at 10 ng
Lane 2: His-tagged mouse SMARCC2/BAF170 recombinant protein at 15 ng
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 123 kDa
Observed band size: 50 kDa
Exposure time: 37s
Western blot - Anti-SMARCC1/BAF155 antibody [EPR25109-77] (ab305037)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Lysates were freshly made and used immediately to minimize protein degradation.

Samples are non-boiled as boiling may cause protein aggregation.

The band beneath the target band (150 kDa) is likely to be degraded target fragments.
Exposure time: 26 seconds
All lanes: Western blot - Anti-SMARCC1/BAF155 antibody [EPR25109-77] (ab305037) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2: 293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg
Lane 3: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg
Lane 4: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 123 kDa
Observed band size: 150 kDa
Exposure time: 26s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCC1/BAF155 antibody [EPR25109-77] (ab305037)
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling SMARCC1/BAF155 with ab305037 at 1/100 (4.75 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Nuclear staining on rat spleen.The section was incubated with ab305037 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCC1/BAF155 antibody [EPR25109-77] (ab305037)
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling SMARCC1/BAF155 with ab305037 at 1/100 (4.75 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Nuclear staining on rat colon.The section was incubated with ab305037 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCC1/BAF155 antibody [EPR25109-77] (ab305037)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling SMARCC1/BAF155 with ab305037 at 1/100 (4.75 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Nuclear staining on mouse spleen.The section was incubated with ab305037 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCC1/BAF155 antibody [EPR25109-77] (ab305037)
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling SMARCC1/BAF155 with ab305037 at 1/100 (4.75 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Nuclear staining on mouse colon.The section was incubated with ab305037 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCC1/BAF155 antibody [EPR25109-77] (ab305037)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SMARCC1/BAF155 with ab305037 at 1/100 (4.75 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Nuclear staining on human kidney (PMID: 33532313).The section was incubated with ab305037 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCC1/BAF155 antibody [EPR25109-77] (ab305037)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SMARCC1/BAF155 with ab305037 at 1/100 (4.75 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Nuclear staining on human colon (PMID:30144500).The section was incubated with ab305037 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCC1/BAF155 antibody [EPR25109-77] (ab305037)
Immunohistochemical analysis of paraffin-embedded (A) Wild-type Hela ( tissue labeling SMARCC1/BAF155 with ab305037 at 1/100 (4.75 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Nuclear staining on (A) Wild-type Hela (human cervix adenocarcinoma epithelial cell) cell pellet, and no staining on (B) SMARCC1 knockout Hela (Human SMARCC1 (BAF155) knockout HeLa cell line ab264859) cell pellet.The section was incubated with ab305037 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunocytochemistry/ Immunofluorescence - Anti-SMARCC1/BAF155 antibody [EPR25109-77] (ab305037)
Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized SMARCC1 KO HeLa (SMARCC1 knockout human cervical adenocarcinoma epithelial cell) (Human SMARCC1 (BAF155) knockout HeLa cell line ab264859) cells labelling SMARCC1/BAF155 with ab305037 at 1/100 (4.75 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing no staining in SMARCC1 knockout Hela cells and showing nuclear staining in wildtype Hela cells.The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8) is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.