JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB224229

Anti-SMARCD1 antibody

Be the first to review this product! Submit a review

|

(3 Publications)

Rabbit Polyclonal SMARCD1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SMARCD1 aa 1-150.

View Alternative Names

BAF60A, SMARCD1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1, 60 kDa BRG-1/Brm-associated factor subunit A, BRG1-associated factor 60A, SWI/SNF complex 60 kDa subunit

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-SMARCD1 antibody (AB224229)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SMARCD1 antibody (AB224229)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for SMARCD1 (green) using ab224229 (4 μg/ml) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCD1 antibody (AB224229)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCD1 antibody (AB224229)

Paraffin-embedded human testis tissue stained for SMARCD1 with ab224229 (1/50) in immunohistochemical analysis.

Western blot - Anti-SMARCD1 antibody (AB224229)
  • WB

Unknown

Western blot - Anti-SMARCD1 antibody (AB224229)

All lanes:

ab224229 at 0.4 µg/mL

All lanes:

Human CACO-2 cell line

Predicted band size: 58 kDa

false

Western blot - Anti-SMARCD1 antibody (AB224229)
  • WB

Supplier Data

Western blot - Anti-SMARCD1 antibody (AB224229)

All lanes:

Western blot - Anti-SMARCD1 antibody (ab224229) at 1/100 dilution

Lane 1:

NIH/3T3 (mouse embyro fibroblast cell line) cell lysate

Lane 2:

NBT-II (rat Wistar bladder tumor cell line) cell lysate

Predicted band size: 58 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SMARCD1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q96GM5

style
left-column

Alternative Products

Primary Antibodies

AB245222

Anti-SMARCD1 antibody [EPR23170-71]

Advanced Validation|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-SMARCD1 antibody [EPR23170-71] (AB245222)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB175933

Anti-53BP1 antibody [EPR2172(2)]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-53BP1 antibody [EPR2172(2)] (AB175933)

  • 5
  • 3
Primary Antibodies

AB133534

Anti-Rad51 antibody [EPR4030(3)]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [EPR4030(3)] (AB133534)

  • 4
  • 15
Primary Antibodies

AB182560

Anti-ARID1A antibody [EPR13501]

Recombinant|KO Validated|Advanced Validation|RabMAb|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-ARID1A antibody [EPR13501] (AB182560)

  • 4
  • 1
Primary Antibodies

AB259839

Anti-BRD9 antibody [EPR23888-5]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD9 antibody [EPR23888-5] (AB259839)

  • 4
  • 1
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMARCD1 also known as SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1 is a component of the SWI/SNF chromatin remodeling complex. The protein has a mass of approximately 50 kDa. It shows expression in various tissues including the brain liver and heart. Alternative names for SMARCD1 include BAF60A indicating its role within the BAF (BRG1/BRM-associated factors) complex.
Biological function summary

SMARCD1 plays a significant role in altering chromatin structure to regulate gene expression. It functions as part of the SWI/SNF complex which modulates access of transcription factors to DNA. This protein contributes to the regulation of various genes involved in cellular processes like proliferation differentiation and DNA repair. The SWI/SNF complex including SMARCD1 alters nucleosome positioning affecting the transcriptional landscape.

Pathways

SMARCD1 influences the expression of genes involved in critical cellular activities. It participates actively in the Wnt signaling and the cell cycle regulation pathways. The Wnt pathway includes critical interactions with proteins like β-catenin which contributes to the regulation of gene transcription related to cell fate and proliferation. SMARCD1's involvement in cell cycle regulation also implicates interactions with proteins such as cyclins and p53.

The altered expression or mutation of SMARCD1 relates to conditions like cancer and developmental disorders. Overexpression of SMARCD1 has associations with certain types of cancers including breast cancer where SMARCD1 modulates interactions with key proteins like BRG1 and BRM. Developmental disorders may involve interactions affecting the SWI/SNF complex leading to issues in tissue differentiation and growth.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner (PubMed : 29374058, PubMed : 8804307). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Has a strong influence on vitamin D-mediated transcriptional activity from an enhancer vitamin D receptor element (VDRE). May be a link between mammalian SWI-SNF-like chromatin remodeling complexes and the vitamin D receptor (VDR) heterodimer (PubMed : 14698202). Mediates critical interactions between nuclear receptors and the BRG1/SMARCA4 chromatin-remodeling complex for transactivation (PubMed : 12917342). Interacts with AKIRIN2 (By similarity).
See full target information SMARCD1
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadx1568 PubMed40712016

2025

Nonchromatin regulatory functions of the histone variant H2A.B in SWI/SNF genomic deposition.

Applications

Unspecified application

Species

Unspecified reactive species

Xuanzhao Jiang,Jiayu Wen,Mary L Nelson,Yasmin Dijkwel,Bradley Cairns,Uta-Maria Bauer,Gene Hart-Smith,Tatiana A Soboleva,David J Tremethick

Cancer discovery 11:2620-2637 PubMed34078620

2021

A Role for SMARCB1 in Synovial Sarcomagenesis Reveals That SS18-SSX Induces Canonical BAF Destruction.

Applications

Unspecified application

Species

Unspecified reactive species

Jinxiu Li,Timothy S Mulvihill,Li Li,Jared J Barrott,Mary L Nelson,Lena Wagner,Ian C Lock,Amir Pozner,Sydney Lynn Lambert,Benjamin B Ozenberger,Michael B Ward,Allie H Grossmann,Ting Liu,Ana Banito,Bradley R Cairns,Kevin B Jones

Cell 173:634-648.e12 PubMed29606356

2018

Tumor-Induced Generation of Splenic Erythroblast-like Ter-Cells Promotes Tumor Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yanmei Han,Qiuyan Liu,Jin Hou,Yan Gu,Yi Zhang,Zhubo Chen,Jia Fan,Weiping Zhou,Shuangjian Qiu,Yonghong Zhang,Tao Dong,Ning Li,Zhengping Jiang,Ha Zhu,Qian Zhang,Yuanwu Ma,Lianfeng Zhang,Qingqing Wang,Yizhi Yu,Nan Li,Xuetao Cao
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com