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Rabbit Polyclonal SMARCD1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SMARCD1 aa 1-150.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human SMARCD1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q96GM5
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Rat
Expected
Tested
Expected
Cow
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/50.00000 - 1/200.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

0.04000-0.40000 µg/mL

Notes

-

Species

Rat

Dilution info

0.04000-0.40000 µg/mL

Notes

-

Species

Human

Dilution info

0.04000-0.40000 µg/mL

Notes

-

Predicted
Predicted

Species

Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

0.25000-2.00000 µg/mL

Notes

Fixation/Permeabilization: PFA/Triton X-100.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Cow

Dilution info

-

Notes

-

Target data

Function

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner (PubMed:8804307, PubMed:29374058). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Has a strong influence on vitamin D-mediated transcriptional activity from an enhancer vitamin D receptor element (VDRE). May be a link between mammalian SWI-SNF-like chromatin remodeling complexes and the vitamin D receptor (VDR) heterodimer (PubMed:14698202). Mediates critical interactions between nuclear receptors and the BRG1/SMARCA4 chromatin-remodeling complex for transactivation (PubMed:12917342).

Alternative names

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Rabbit Polyclonal SMARCD1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SMARCD1 aa 1-150.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human SMARCD1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q96GM5
Purification technique

Affinity purification Immunogen

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

SMARCD1 plays a significant role in altering chromatin structure to regulate gene expression. It functions as part of the SWI/SNF complex which modulates access of transcription factors to DNA. This protein contributes to the regulation of various genes involved in cellular processes like proliferation differentiation and DNA repair. The SWI/SNF complex including SMARCD1 alters nucleosome positioning affecting the transcriptional landscape.

Activity summary

SMARCD1 also known as SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1 is a component of the SWI/SNF chromatin remodeling complex. The protein has a mass of approximately 50 kDa. It shows expression in various tissues including the brain liver and heart. Alternative names for SMARCD1 include BAF60A indicating its role within the BAF (BRG1/BRM-associated factors) complex.

Pathways

SMARCD1 influences the expression of genes involved in critical cellular activities. It participates actively in the Wnt signaling and the cell cycle regulation pathways. The Wnt pathway includes critical interactions with proteins like β-catenin which contributes to the regulation of gene transcription related to cell fate and proliferation. SMARCD1's involvement in cell cycle regulation also implicates interactions with proteins such as cyclins and p53.

Associated diseases and disorders

The altered expression or mutation of SMARCD1 relates to conditions like cancer and developmental disorders. Overexpression of SMARCD1 has associations with certain types of cancers including breast cancer where SMARCD1 modulates interactions with key proteins like BRG1 and BRM. Developmental disorders may involve interactions affecting the SWI/SNF complex leading to issues in tissue differentiation and growth.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-SMARCD1 antibody (ab224229), expandable thumbnail

    Western blot - Anti-SMARCD1 antibody (ab224229)

    All lanes: ab224229 at 0.4 µg/mL

    All lanes: Human CACO-2 cell line

    Predicted band size: 58 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCD1 antibody (ab224229), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCD1 antibody (ab224229)

    Paraffin-embedded human testis tissue stained for SMARCD1 with ab224229 (1/50) in immunohistochemical analysis.

  • Western blot - Anti-SMARCD1 antibody (ab224229), expandable thumbnail

    Western blot - Anti-SMARCD1 antibody (ab224229)

    All lanes: Western blot - Anti-SMARCD1 antibody (AB224229) at 1/100 dilution

    Lane 1: NIH/3T3 (mouse embyro fibroblast cell line) cell lysate

    Lane 2: NBT-II (rat Wistar bladder tumor cell line) cell lysate

    Predicted band size: 58 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-SMARCD1 antibody (ab224229), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SMARCD1 antibody (ab224229)

    PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for SMARCD1 (green) using ab224229 (4 μg/ml) in ICC/IF.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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