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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit Polyclonal SMARCD1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SMARCD1 aa 1-150.
IgG
Rabbit
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
IHC-P | WB | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Expected | Tested | Expected |
Rat | Expected | Tested | Expected |
Cow | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Rat | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow | Dilution info - | Notes - |
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner (PubMed:8804307, PubMed:29374058). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Has a strong influence on vitamin D-mediated transcriptional activity from an enhancer vitamin D receptor element (VDRE). May be a link between mammalian SWI-SNF-like chromatin remodeling complexes and the vitamin D receptor (VDR) heterodimer (PubMed:14698202). Mediates critical interactions between nuclear receptors and the BRG1/SMARCA4 chromatin-remodeling complex for transactivation (PubMed:12917342).
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1, 60 kDa BRG-1/Brm-associated factor subunit A, BRG1-associated factor 60A, SWI/SNF complex 60 kDa subunit, BAF60A, BAF60A, SMARCD1
Rabbit Polyclonal SMARCD1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SMARCD1 aa 1-150.
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1, 60 kDa BRG-1/Brm-associated factor subunit A, BRG1-associated factor 60A, SWI/SNF complex 60 kDa subunit, BAF60A, BAF60A, SMARCD1
IgG
Rabbit
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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SMARCD1 plays a significant role in altering chromatin structure to regulate gene expression. It functions as part of the SWI/SNF complex which modulates access of transcription factors to DNA. This protein contributes to the regulation of various genes involved in cellular processes like proliferation differentiation and DNA repair. The SWI/SNF complex including SMARCD1 alters nucleosome positioning affecting the transcriptional landscape.
SMARCD1 also known as SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1 is a component of the SWI/SNF chromatin remodeling complex. The protein has a mass of approximately 50 kDa. It shows expression in various tissues including the brain liver and heart. Alternative names for SMARCD1 include BAF60A indicating its role within the BAF (BRG1/BRM-associated factors) complex.
SMARCD1 influences the expression of genes involved in critical cellular activities. It participates actively in the Wnt signaling and the cell cycle regulation pathways. The Wnt pathway includes critical interactions with proteins like β-catenin which contributes to the regulation of gene transcription related to cell fate and proliferation. SMARCD1's involvement in cell cycle regulation also implicates interactions with proteins such as cyclins and p53.
The altered expression or mutation of SMARCD1 relates to conditions like cancer and developmental disorders. Overexpression of SMARCD1 has associations with certain types of cancers including breast cancer where SMARCD1 modulates interactions with key proteins like BRG1 and BRM. Developmental disorders may involve interactions affecting the SWI/SNF complex leading to issues in tissue differentiation and growth.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
Terms & Conditions.
All lanes: ab224229 at 0.4 µg/mL
All lanes: Human CACO-2 cell line
Predicted band size: 58 kDa
Paraffin-embedded human testis tissue stained for SMARCD1 with ab224229 (1/50) in immunohistochemical analysis.
All lanes: Western blot - Anti-SMARCD1 antibody (AB224229) at 1/100 dilution
Lane 1: NIH/3T3 (mouse embyro fibroblast cell line) cell lysate
Lane 2: NBT-II (rat Wistar bladder tumor cell line) cell lysate
Predicted band size: 58 kDa
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for SMARCD1 (green) using ab224229 (4 μg/ml) in ICC/IF.
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