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AB221168

Anti-SMARCD2 antibody

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(1 Publication)

Rabbit Polyclonal SMARCD2 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SMARCD2 aa 50-150.

View Alternative Names

BAF60B, PRO2451, SMARCD2, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 2, 60 kDa BRG-1/Brm-associated factor subunit B, BRG1-associated factor 60B

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-SMARCD2 antibody (AB221168)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SMARCD2 antibody (AB221168)

Immunofluorescent analysis of PFA-fixed/Triton X-100 permeabilized MCF7 cells labeling SMARCD2 with ab221168 at 4 μg/ml (green).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SMARCD2 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

Q92925

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SMARCD2 protein also known as BAF60B functions as a subunit of the SWI/SNF chromatin remodeling complex. It possesses a molecular mass of approximately 60 kDa. The protein is widely expressed with notable levels observed in hematopoietic tissues and the central nervous system. SMARCD2 contributes to the modulation of chromatin structure thereby facilitating accessibility of transcriptional machinery to DNA.
Biological function summary

SMARCD2 plays a significant role in the regulation of transcription. As a component of the chromatin remodeling complex it influences gene expression linked to differentiation and development. The SWI/SNF complex which includes SMARCD2 modulates nucleosome positioning on DNA therefore impacting cellular responses to various signaling pathways. The regulatory effects of SMARCD2 within the complex are important for proper lineage commitment and cell fate decisions.

Pathways

SMARCD2 interacts within the context of transcriptional regulation. It is notably involved in the Wnt signaling pathway and the Notch signaling pathway. In these pathways SMARCD2 interacts with proteins like beta-catenin and RBPJ directly influencing the transcription of downstream target genes. SMARCD2's activity within these signaling modules contributes to the regulation of proliferation and differentiation processes.

SMARCD2 is linked to acute myeloid leukemia and neurodevelopmental disorders. Aberrations in the SWI/SNF complex including altered SMARCD2 function can lead to disrupted gene regulation and contribute to leukemogenesis. Similarly mutations affecting SMARCD2 are associated with neurodevelopmental syndromes such as Coffin-Siris syndrome. In these contexts the interplay between SMARCD2 and other complex members such as SMARCA4 influences disease pathogenesis and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner (PubMed : 22952240, PubMed : 26601204). Critical regulator of myeloid differentiation, controlling granulocytopoiesis and the expression of genes involved in neutrophil granule formation (PubMed : 28369036).
See full target information SMARCD2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 222: PubMed36880596

2023

The p97/VCP segregase is essential for arsenic-induced degradation of PML and PML-RARA.

Applications

Unspecified application

Species

Unspecified reactive species

Ellis G Jaffray,Michael H Tatham,Barbara Mojsa,Magda Liczmanska,Alejandro Rojas-Fernandez,Yili Yin,Graeme Ball,Ronald T Hay
View all publications
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Product promise

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