JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB256304

Anti-SMARCD2 antibody [EPR20860-251] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal SMARCD2 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human, Rat samples. Cited in 1 publication.

View Alternative Names

BAF60B, PRO2451, SMARCD2, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 2, 60 kDa BRG-1/Brm-associated factor subunit B, BRG1-associated factor 60B

5 Images
Flow Cytometry (Intracellular) - Anti-SMARCD2 antibody [EPR20860-251] - BSA and Azide free (AB256304)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-SMARCD2 antibody [EPR20860-251] - BSA and Azide free (AB256304)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cell line labeling SMARCD2 with ab220164 at 1/600 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue).

Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220164).

Immunoprecipitation - Anti-SMARCD2 antibody [EPR20860-251] - BSA and Azide free (AB256304)
  • IP

Unknown

Immunoprecipitation - Anti-SMARCD2 antibody [EPR20860-251] - BSA and Azide free (AB256304)

SMARCD2 was immunoprecipitated from 0.35 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab220164 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220164 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.

Lane 1 : HeLa whole cell lysate 10 μg (Input).
Lane 2 : ab220164 IP in HeLa whole cell lysate .
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab220164 in HeLa whole cell lysate.

Blocking/Dilution buffer : 5% NFDM/TBST.
Exposure time : 40 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220164).

All lanes:

Immunoprecipitation - Anti-SMARCD2 antibody [EPR20860-251] (<a href='/en-us/products/primary-antibodies/smarcd2-antibody-epr20860-251-ab220164'>ab220164</a>)

Predicted band size: 52 kDa

Observed band size: 59 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCD2 antibody [EPR20860-251] - BSA and Azide free (AB256304)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCD2 antibody [EPR20860-251] - BSA and Azide free (AB256304)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling SMARCD2 with ab220164 at 1/200 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse colon (PMID : 20148946, 28303890, 25622893). The section was incubated with ab220164 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220164).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCD2 antibody [EPR20860-251] - BSA and Azide free (AB256304)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCD2 antibody [EPR20860-251] - BSA and Azide free (AB256304)

Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling SMARCD2 with ab220164 at 1/200 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on rat testis (PMID : 20148946, 28303890, 25622893). The section was incubated with ab220164 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220164).

Immunoprecipitation - Anti-SMARCD2 antibody [EPR20860-251] - BSA and Azide free (AB256304)
  • IP

Supplier Data

Immunoprecipitation - Anti-SMARCD2 antibody [EPR20860-251] - BSA and Azide free (AB256304)

SMARCD2 was immunoprecipitated from 0.35 mg mouse P70 thymus lysate with ab220164 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220164 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.

Lane 1 : Mouse P70 thymus lysate 10 μg (Input).
Lane 2 : ab220164 IP in mouse P70 thymus lysate .
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab220164 in mouse P70 thymus lysate.

Blocking/Dilution buffer : 5% NFDM/TBST.
Exposure time : 3 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220164).

All lanes:

Immunoprecipitation - Anti-SMARCD2 antibody [EPR20860-251] (<a href='/en-us/products/primary-antibodies/smarcd2-antibody-epr20860-251-ab220164'>ab220164</a>) at 1/1000 dilution

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Predicted band size: 52 kDa

Observed band size: 59 kDa

false

Exposure time: 3s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20860-251

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IP, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

IHC is recommended only for mouse and rat.

style
left-column

Alternative Products

Primary Antibodies

AB221168

Anti-SMARCD2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-SMARCD2 antibody (AB221168)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB260043

Anti-Collagen I antibody [EPR22894-89]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-Collagen I antibody [EPR22894-89] (AB260043)

  • 4
  • 3
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
Primary Antibodies

AB172638

Anti-SMARCC1/BAF155 antibody [EPR12395] - ChIP Grade

20ul selling size|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-SMARCC1/BAF155 antibody [EPR12395] - ChIP Grade (AB172638)

  • 5
  • 2
Primary Antibodies

AB209845

Anti-GSDMD antibody [EPR19828]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-GSDMD antibody [EPR19828] (AB209845)

  • 4
  • 3
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
style
left-column

Product details

ab256304 is the carrier-free version of ab220164.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SMARCD2 protein also known as BAF60B functions as a subunit of the SWI/SNF chromatin remodeling complex. It possesses a molecular mass of approximately 60 kDa. The protein is widely expressed with notable levels observed in hematopoietic tissues and the central nervous system. SMARCD2 contributes to the modulation of chromatin structure thereby facilitating accessibility of transcriptional machinery to DNA.
Biological function summary

SMARCD2 plays a significant role in the regulation of transcription. As a component of the chromatin remodeling complex it influences gene expression linked to differentiation and development. The SWI/SNF complex which includes SMARCD2 modulates nucleosome positioning on DNA therefore impacting cellular responses to various signaling pathways. The regulatory effects of SMARCD2 within the complex are important for proper lineage commitment and cell fate decisions.

Pathways

SMARCD2 interacts within the context of transcriptional regulation. It is notably involved in the Wnt signaling pathway and the Notch signaling pathway. In these pathways SMARCD2 interacts with proteins like beta-catenin and RBPJ directly influencing the transcription of downstream target genes. SMARCD2's activity within these signaling modules contributes to the regulation of proliferation and differentiation processes.

SMARCD2 is linked to acute myeloid leukemia and neurodevelopmental disorders. Aberrations in the SWI/SNF complex including altered SMARCD2 function can lead to disrupted gene regulation and contribute to leukemogenesis. Similarly mutations affecting SMARCD2 are associated with neurodevelopmental syndromes such as Coffin-Siris syndrome. In these contexts the interplay between SMARCD2 and other complex members such as SMARCA4 influences disease pathogenesis and progression.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner (PubMed : 22952240, PubMed : 26601204). Critical regulator of myeloid differentiation, controlling granulocytopoiesis and the expression of genes involved in neutrophil granule formation (PubMed : 28369036).
See full target information SMARCD2
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cardiovascular medicine 9:1013262 PubMed36684586

2023

SARS-CoV-2 Spike protein activates TMEM16F-mediated platelet procoagulant activity.

Applications

Unspecified application

Species

Unspecified reactive species

Ambra Cappelletto,Harriet E Allan,Marilena Crescente,Edoardo Schneider,Rossana Bussani,Hashim Ali,Ilaria Secco,Simone Vodret,Roberto Simeone,Luca Mascaretti,Serena Zacchigna,Timothy D Warner,Mauro Giacca
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com