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AB327181

Anti-SMARCE1 Antibody

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Rabbit Polyclonal BAF57/SMARCE1 antibody. Suitable for WB, IHC-P, ICC/IF, ChIP and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human SMARCE1.

View Alternative Names

BAF57, SMARCE1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1, BRG1-associated factor 57, Baf57, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1, BRG1-associated factor 57, BAF57, Baf57, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1, BRG1-associated factor 57, BAF57

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCE1 Antibody (AB327181)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCE1 Antibody (AB327181)

Immunohistochemical staining of human endometrium using ab327181 at 1 : 200–1 : 500 dilution, showing high expression.

Immunocytochemistry/ Immunofluorescence - Anti-SMARCE1 Antibody (AB327181)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SMARCE1 Antibody (AB327181)

Immunofluorescent staining of human cell line U-2 OS using ab327181 at 0.25–2 µg/ml, showing localization to nucleoplasm.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCE1 Antibody (AB327181)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCE1 Antibody (AB327181)

Immunohistochemical staining of human liver using ab327181 at 1 : 200–1 : 500 dilution, showing low expression as expected.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCE1 Antibody (AB327181)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCE1 Antibody (AB327181)

Immunohistochemistry analysis in human endometrium and liver tissues using ab327181 at 1 : 200–1 : 500 dilution. Corresponding SMARCE1 RNA-seq data are presented for the same tissues.

ChIP - Anti-SMARCE1 Antibody (AB327181)
  • ChIP

Supplier Data

ChIP - Anti-SMARCE1 Antibody (AB327181)

ChIP-Exo-Seq composite graph for ab327181 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Western blot - Anti-SMARCE1 Antibody (AB327181)
  • WB

Supplier Data

Western blot - Anti-SMARCE1 Antibody (AB327181)

Western blot analysis in human cell line MOLT-4 using ab327181 at 0.04–0.4 µg/ml.

All lanes:

Western blot - Anti-SMARCE1 Antibody (ab327181)

All lanes:

Human cell line MOLT-4

Predicted band size: 46.65 kDa

false

Western blot - Anti-SMARCE1 Antibody (AB327181)
  • WB

Supplier Data

Western blot - Anti-SMARCE1 Antibody (AB327181)

Western blot analysis in mouse cell line NIH-3T3 and rat cell line NBT-II using ab327181 at 0.04–0.4 µg/ml.

All lanes:

Western blot - Anti-SMARCE1 Antibody (ab327181)

Lane 2:

Mouse cell line NIH-3T3

Lane 3:

Rat cell line NBT-II

Predicted band size: 46.65 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

ChIP, WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SMARCE1.

Q969G3

Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Required for the coactivation of estrogen responsive promoters by SWI/SNF complexes and the SRC/p160 family of histone acetyltransferases (HATs). Also specifically interacts with the CoREST corepressor resulting in repression of neuronal specific gene promoters in non-neuronal cells.
See full target information SMARCE1

Additional targets

,

Product promise

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