Rabbit Polyclonal SMC1A antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SMC1A aa 700-1050.
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Publications
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- Nature communications 12:51232021OCT4 cooperates with distinct ATP-dependent chromatin remodelers in naïve and primed pluripotent states in human.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Huang,Kyoung-Mi Park,Paul Gontarz,Bo Zhang,Joshua Pan,Zachary McKenzie,Laura A Fischer,Chen Dong,Sabine Dietmann,Xiaoyun Xing,Pavel V Shliaha,Jihong Yang,Dan Li,Junjun Ding,Tenzin Lungjangwa,Maya Mitalipova,Shafqat A Khan,Sumeth Imsoonthornruksa,Nick Jensen,Ting Wang,Cigall Kadoch,Rudolf Jaenisch,Jianlong Wang,Thorold W TheunissenPubMed 34446700
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SMC1A aa 700-1050. The exact immunogen used to generate this antibody is proprietary information. Database link Q14683
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Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Xenopus laevis | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Xenopus laevis | Dilution info - | Notes - |
Associated Products
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Target data
Function
Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.
Alternative names
DXS423E, KIAA0178, SB1.8, SMC1, SMC1L1, SMC1A, Structural maintenance of chromosomes protein 1A, SMC protein 1A, SMC-1-alpha, SMC-1A, Sb1.8
Recommended products
Rabbit Polyclonal SMC1A antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SMC1A aa 700-1050.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SMC1A aa 700-1050. The exact immunogen used to generate this antibody is proprietary information. Database link Q14683
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SMC1A also known as structural maintenance of chromosomes 1A is an essential component of the cohesin complex. This complex regulates the cohesion of sister chromatids ensuring accurate chromosome segregation during cell division. With a molecular mass of around 143 kDa SMC1A is expressed ubiquitously with higher levels in proliferating cells. It is a member of the SMC protein family possessing distinct N-terminal and C-terminal domains connected by a flexible coiled-coil region.
- Biological function summary
SMC1A forms a complex with other proteins most notably SMC3 RAD21 and STAG2. This complex is necessary for maintaining chromatid cohesion during the cell cycle's S and G2 phases. Furthermore SMC1A influences DNA repair specifically homologous recombination by maintaining genome stability. Through its associations SMC1A contributes to regulation of gene expression further illustrating its multifunctional role in cellular processes.
- Pathways
SMC1A integrates into DNA damage response and repair pathways. The ATR and ATM signaling pathways interact with SMC1A modulating its function during DNA repair mechanisms. SMC1A associates with proteins like PDS5 and WAPL which influence cohesin dynamics. These interactions stress SMC1A’s role in preserving genomic integrity and controlling cell cycle progression.
- Associated diseases and disorders
Mutations or deregulation of SMC1A links to Cornelia de Lange Syndrome and certain cancers. This role is particularly significant in Cornelia de Lange Syndrome where SMC1A mutations disrupt normal chromosomal condensation and separation. Within oncogenesis SMC1A's interaction with TP53 indicates a broader influence on tumorigenesis emphasizing its involvement in maintaining cellular homeostasis and preventing cancer progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-SMC1A antibody (ab137707)
All lanes: Western blot - Anti-SMC1A antibody (ab137707) at 1/1000 dilution
Lane 1: A431 whole cell lysate at 30 µg
Lane 2: H1299 whole cell lysate at 30 µg
Predicted band size: 143 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A antibody (ab137707)
Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft labelling SMC1A with ab137707 at 1/500.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-SMC1A antibody (ab137707)
SMC1A western blot using anti-SMC1A antibody ab137707. Publication image and figure legend from Huang, X., Park, K. M., et al., 2021, Nat Commun, PubMed 34446700.
ab137707 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab137707 please see the product overview.
An OCT4 interactome in human embryonic stem cells (hESCs).a Affinity purification mass spectrometry (AP-MS) strategies for identification of OCT4-associated proteins (interactome) in hESCs. Three strategies are used: (i) streptavidin immunoprecipitation (SA-IP) of WIBR2-OCT43xFLBio ESCs or BirA control; (ii) FLAG-IP of WIBR2-OCT43xFLBio ESCs or BirA control; (iii) native OCT4 and IgG antibody (Ab-IP) pulldown in WIBR2 and WIBR3 hESC lines. b OCT4-associated proteins identified in naïve and primed hESCs. Proteins are listed as official gene symbols. c Proteins in the naïve human OCT4 interactome are compared to OCT4-associated proteins identified in three independent studies in mouse ESCs29–31. Components of SNF2-family chromatin remodeling complexes are underlined. d OCT4 co-immunoprecipitation (co-IP) followed by western blot of selected OCT4-associated proteins in naïve and primed hESCs. e BRM (SMARCA2) and BRG1 (SMARCA4) co-IP followed by western blot analysis of BAF155 (SMARCC1) and OCT4. d, e Experiment is repeated independently twice with similar results.
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