AB137707

Anti-SMC1A antibody

Name: Anti-SMC1A antibody (ab137707) | Abcam
Brand: Abcam Limited
SKU: AB137707
Price: 535 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal SMC1A antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SMC1A aa 700-1050.

View Alternative Names

DXS423E, KIAA0178, SB1.8, SMC1, SMC1L1, SMC1A, Structural maintenance of chromosomes protein 1A, SMC protein 1A, SMC-1-alpha, SMC-1A, Sb1.8

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A antibody (AB137707)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A antibody (AB137707)

Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft labelling SMC1A with ab137707 at 1/500.

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Western blot - Anti-SMC1A antibody (AB137707)

All lanes:

Western blot - Anti-SMC1A antibody (ab137707) at 1/1000 dilution

Lane 1:

A431 whole cell lysate at 30 µg

Lane 2:

H1299 whole cell lysate at 30 µg

Predicted band size: 143 kDa

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CiteAb

Western blot - Anti-SMC1A antibody (AB137707)

SMC1A western blot using anti-SMC1A antibody ab137707. Publication image and figure legend from Huang, X., Park, K. M., et al., 2021, Nat Commun, PubMed 34446700.

ab137707 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab137707 please see the product overview.

An OCT4 interactome in human embryonic stem cells (hESCs).a Affinity purification mass spectrometry (AP-MS) strategies for identification of OCT4-associated proteins (interactome) in hESCs. Three strategies are used : (i) streptavidin immunoprecipitation (SA-IP) of WIBR2-OCT43xFLBio ESCs or BirA control; (ii) FLAG-IP of WIBR2-OCT43xFLBio ESCs or BirA control; (iii) native OCT4 and IgG antibody (Ab-IP) pulldown in WIBR2 and WIBR3 hESC lines. b OCT4-associated proteins identified in naïve and primed hESCs. Proteins are listed as official gene symbols. c Proteins in the naïve human OCT4 interactome are compared to OCT4-associated proteins identified in three independent studies in mouse ESCs29–31. Components of SNF2-family chromatin remodeling complexes are underlined. d OCT4 co-immunoprecipitation (co-IP) followed by western blot of selected OCT4-associated proteins in naïve and primed hESCs. e BRM (SMARCA2) and BRG1 (SMARCA4) co-IP followed by western blot analysis of BAF155 (SMARCC1) and OCT4. d, e Experiment is repeated independently twice with similar results.

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human SMC1A aa 700-1050. The exact immunogen used to generate this antibody is proprietary information.

Q14683

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMC1A also known as structural maintenance of chromosomes 1A is an essential component of the cohesin complex. This complex regulates the cohesion of sister chromatids ensuring accurate chromosome segregation during cell division. With a molecular mass of around 143 kDa SMC1A is expressed ubiquitously with higher levels in proliferating cells. It is a member of the SMC protein family possessing distinct N-terminal and C-terminal domains connected by a flexible coiled-coil region.

Biological function summary

SMC1A forms a complex with other proteins most notably SMC3 RAD21 and STAG2. This complex is necessary for maintaining chromatid cohesion during the cell cycle's S and G2 phases. Furthermore SMC1A influences DNA repair specifically homologous recombination by maintaining genome stability. Through its associations SMC1A contributes to regulation of gene expression further illustrating its multifunctional role in cellular processes.

Pathways

SMC1A integrates into DNA damage response and repair pathways. The ATR and ATM signaling pathways interact with SMC1A modulating its function during DNA repair mechanisms. SMC1A associates with proteins like PDS5 and WAPL which influence cohesin dynamics. These interactions stress SMC1A’s role in preserving genomic integrity and controlling cell cycle progression.

Mutations or deregulation of SMC1A links to Cornelia de Lange Syndrome and certain cancers. This role is particularly significant in Cornelia de Lange Syndrome where SMC1A mutations disrupt normal chromosomal condensation and separation. Within oncogenesis SMC1A's interaction with TP53 indicates a broader influence on tumorigenesis emphasizing its involvement in maintaining cellular homeostasis and preventing cancer progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.

See full target information SMC1A

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:5261 PubMed40480980

2025

TANGO2 binds crystallin alpha B and its loss causes desminopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Maike Stentenbach,Laetitia A Hughes,Samuel V Fagan,Blake Payne,Danielle L Rudler,Stefan J Siira,Tim McCubbin,Anaëlle Chopin,Kara L Perks,Judith A Ermer,James Hendry,Teagan S Er,Shanti Balasubramaniam,Joel A Eliades,Livia C Hool,Nicolle H Packer,Edward S X Moh,Benjamin S Padman,Oliver Rackham,Aleksandra Filipovska

Nature communications 12:5123 PubMed34446700

2021

OCT4 cooperates with distinct ATP-dependent chromatin remodelers in naïve and primed pluripotent states in human.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Huang,Kyoung-Mi Park,Paul Gontarz,Bo Zhang,Joshua Pan,Zachary McKenzie,Laura A Fischer,Chen Dong,Sabine Dietmann,Xiaoyun Xing,Pavel V Shliaha,Jihong Yang,Dan Li,Junjun Ding,Tenzin Lungjangwa,Maya Mitalipova,Shafqat A Khan,Sumeth Imsoonthornruksa,Nick Jensen,Ting Wang,Cigall Kadoch,Rudolf Jaenisch,Jianlong Wang,Thorold W Theunissen

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-SMC1A antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A antibody (AB137707)

Western blot - Anti-SMC1A antibody (AB137707)

Western blot - Anti-SMC1A antibody (AB137707)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise