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Rabbit Polyclonal SMC1A antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human samples. Cited in 17 publications.


Images

Immunoprecipitation - Anti-SMC1A antibody (AB21583), expandable thumbnail
  • Western blot - Anti-SMC1A antibody (AB21583), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A antibody (AB21583), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SMC1A antibody (AB21583), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IF
Human
Tested
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Predicted
Chicken
Predicted
Predicted
Predicted
Predicted
Xenopus laevis
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1 µg/mL

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse, Rat, Chicken, Xenopus laevis

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Chicken, Xenopus laevis

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1 µg/mL

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Chicken, Xenopus laevis

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1 µg/mL

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Chicken, Xenopus laevis

Dilution info

-

Notes

-

Associated Products

Select an associated product type

9 products for Alternative Product

Target data

Function

Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.

Alternative names

Recommended products

Rabbit Polyclonal SMC1A antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human samples. Cited in 17 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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4 product images

  • Immunoprecipitation - Anti-SMC1A antibody (ab21583), expandable thumbnail

    Immunoprecipitation - Anti-SMC1A antibody (ab21583)

    SMC1A was immunoprecipitated using 0.5mg Hela whole cell extract, 5μg of Rabbit polyclonal to SMC1A and 50μl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40μl SDS loading buffer and incubated for 10min at 70oC; 10μl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab21583.
    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
    Band: 150kDa: SMC1A; Non specific - 41 and 42kDa: We are unsure as to the identity of this extra band.

    All lanes: Immunoprecipitation - Anti-SMC1A antibody (ab21583)

    Predicted band size: 143 kDa

  • Western blot - Anti-SMC1A antibody (ab21583), expandable thumbnail

    Western blot - Anti-SMC1A antibody (ab21583)

    ab21583 detects a band of 150 kDa, corresponding to the size of SMC1A. This band is competed away by the addition of the immunizing peptide, showing that it is a specific interaction.

    All lanes: Western blot - Anti-SMC1A antibody (ab21583) at 1 µg/mL

    Lanes 1 and 3: 20ug HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lanes 2 and 4: Western blot - Jurkat whole cell lysate (Jurkat whole cell lysate ab7899) at 20 µg

    Secondary

    All lanes: Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) (ab28446) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 143 kDa

    Observed band size: 150 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A antibody (ab21583), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A antibody (ab21583)

    IHC image of SMC1A staining in human skin FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab21583, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • Immunocytochemistry/ Immunofluorescence - Anti-SMC1A antibody (ab21583), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SMC1A antibody (ab21583)

    ab21583 staining SMC1A in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab21583 at 1µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Also suitable in cells fixed with 4% paraformaldehyde (10 min).Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

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Product protocols

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