Rabbit Polyclonal SMC1A antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse samples. Cited in 41 publications. Immunogen corresponding to Synthetic Peptide within Human SMC1A aa 1100 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
Images
Publications
Filter by
- Genome biology 23:702022Meiosis-specific cohesin complexes display essential and distinct roles in mitotic embryonic stem cell chromosomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEui-Hwan Choi,Seobin Yoon,Young Eun Koh,Tae Kyung Hong,Jeong Tae Do,Bum-Kyu Lee,Yoonsoo Hahn,Keun P KimPubMed 35241136
- Nature communications 13:1872022The three-dimensional structure of Epstein-Barr virus genome varies by latency type and is regulated by PARP1 enzymatic activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah M Morgan,Hideki Tanizawa,Lisa Beatrice Caruso,Michael Hulse,Andrew Kossenkov,Jozef Madzo,Kelsey Keith,Yinfei Tan,Sarah Boyle,Paul M Lieberman,Italo TemperaPubMed 35039491
- Cell death and differentiation 29:687-6962021RNA-interference screen for p53 regulators unveils a role of WDR75 in ribosome biogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPavel Moudry,Katarina Chroma,Sladana Bursac,Sinisa Volarevic,Jiri BartekPubMed 34611297
- Life science alliance 4:2021Paralogous synthetic lethality underlies genetic dependencies of the cancer-mutated gene .Applications:Unspecified applicationReactive species:Unspecified reactive speciesMelanie L Bailey,David Tieu,Andrea Habsid,Amy Hin Yan Tong,Katherine Chan,Jason Moffat,Philip HieterPubMed 34462321
- Journal of atherosclerosis and thrombosis 29:200-2202021MicroRNA-320b Modulates Cholesterol Efflux and Atherosclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaomei Lu,Bin Yang,Huijun Yang,Laiyuan Wang,Hongfan Li,Shufeng Chen,Xiangfeng Lu,Dongfeng GuPubMed 33536383
- Virology journal 18:262021Cohesin promotes HSV-1 lytic transcription by facilitating the binding of RNA Pol II on viral genes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Li et. al.PubMed 33485391
- Nucleic acids research 48:12135-121502020Generation of onco-enhancer enhances chromosomal remodeling and accelerates tumorigenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeiwei Chai,Jie Yu,Ruobing Jia,Xuyang Wen,Tianyi Ding,Xiaoyu Zhang,Hongyan Ni,Renbing Jia,Shengfang Ge,He Zhang,Xianqun FanPubMed 33196849
- The Journal of cell biology 219:2020Tex19.1 inhibits the N-end rule pathway and maintains acetylated SMC3 cohesin and sister chromatid cohesion in oocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJudith Reichmann,Karen Dobie,Lisa M Lister,James H Crichton,Diana Best,Marie MacLennan,David Read,Eleanor S Raymond,Chao-Chun Hung,Shelagh Boyle,Katsuhiko Shirahige,Howard J Cooke,Mary Herbert,Ian R AdamsPubMed 32232464
- Nucleic acids research 48:3935-39482020Oplr16 serves as a novel chromatin factor to control stem cell fate by modulating pluripotency-specific chromosomal looping and TET2-mediated DNA demethylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLin Jia,Yichen Wang,Cong Wang,Zhonghua Du,Shilin Zhang,Xue Wen,Lei Zhou,Hui Li,Huiling Chen,Dan Li,Songling Zhang,Wei Li,Wei Xu,Andrew R Hoffman,Jiuwei Cui,Ji-Fan HuPubMed 32055844
- Oncology reports :2019SMC1 promotes proliferation and inhibits apoptosis through the NF‑κB signaling pathway in colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJin Li et. al.PubMed 31524239
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SMC1A aa 1100 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q14683
Consider this alternative
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested |
| Rat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/2000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-4 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.
Alternative names
DXS423E, KIAA0178, SB1.8, SMC1, SMC1L1, SMC1A, Structural maintenance of chromosomes protein 1A, SMC protein 1A, SMC-1-alpha, SMC-1A, Sb1.8
Recommended products
Rabbit Polyclonal SMC1A antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse samples. Cited in 41 publications. Immunogen corresponding to Synthetic Peptide within Human SMC1A aa 1100 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SMC1A aa 1100 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q14683
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280nm of 1.4 equals 1.0 mg of IgG.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SMC1A also known as structural maintenance of chromosomes 1A is an essential component of the cohesin complex. This complex regulates the cohesion of sister chromatids ensuring accurate chromosome segregation during cell division. With a molecular mass of around 143 kDa SMC1A is expressed ubiquitously with higher levels in proliferating cells. It is a member of the SMC protein family possessing distinct N-terminal and C-terminal domains connected by a flexible coiled-coil region.
- Biological function summary
SMC1A forms a complex with other proteins most notably SMC3 RAD21 and STAG2. This complex is necessary for maintaining chromatid cohesion during the cell cycle's S and G2 phases. Furthermore SMC1A influences DNA repair specifically homologous recombination by maintaining genome stability. Through its associations SMC1A contributes to regulation of gene expression further illustrating its multifunctional role in cellular processes.
- Pathways
SMC1A integrates into DNA damage response and repair pathways. The ATR and ATM signaling pathways interact with SMC1A modulating its function during DNA repair mechanisms. SMC1A associates with proteins like PDS5 and WAPL which influence cohesin dynamics. These interactions stress SMC1A’s role in preserving genomic integrity and controlling cell cycle progression.
- Associated diseases and disorders
Mutations or deregulation of SMC1A links to Cornelia de Lange Syndrome and certain cancers. This role is particularly significant in Cornelia de Lange Syndrome where SMC1A mutations disrupt normal chromosomal condensation and separation. Within oncogenesis SMC1A's interaction with TP53 indicates a broader influence on tumorigenesis emphasizing its involvement in maintaining cellular homeostasis and preventing cancer progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Western blot - Anti-SMC1A antibody (ab9262)
Detection: Chemiluminescence.
All lanes: Western blot - Anti-SMC1A antibody (ab9262) at 0.1 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: 293T whole cell lysate at 50 µg
Lane 3: NIH3T3 whole cell lysate at 50 µg
Predicted band size: 143 kDa
Exposure time: 3s
Immunoprecipitation - Anti-SMC1A antibody (ab9262)
SMC1A was immunoprecipitated from 1 mg HeLa whole cell lysate with ab9262 at 6 μg per reaction. Western blot was performed on the immunoprecipitate using Anti-SMC1A antibody [BL-205-2G8] ab243875 at 1/1000 dilution.
Lysates prepared using NETN lysis buffer.
Lane 1: ab9262 IP in HeLa whole cell lysate.
Lane 2: Contol IgG in HeLa whole cell lysate.
Detection: Chemiluminescence.
Exposure time: 10 seconds.
All lanes: Immunoprecipitation - Anti-SMC1A antibody (ab9262)
Predicted band size: 143 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A antibody (ab9262)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian cancer tissue, labeling SMC1 with ab9262 at 0.2 μg/mL. Detection: DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A antibody (ab9262)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse colon cancer tissue CT26, labeling SMC1 with ab9262 at 0.2 μg/mL. Detection: DAB.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com