AB243875

Anti-SMC1A antibody [BL-205-2G8]

Recombinant
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(3 Publications)

Rabbit Recombinant Monoclonal SMC1A antibody. Suitable for IHC-P, ICC, IP, WB, Flow Cyt and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human SMC1A aa 1200 to C-terminus.

View Alternative Names

DXS423E, KIAA0178, SB1.8, SMC1, SMC1L1, SMC1A, Structural maintenance of chromosomes protein 1A, SMC protein 1A, SMC-1-alpha, SMC-1A, Sb1.8

10 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A antibody [BL-205-2G8] (AB243875)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A antibody [BL-205-2G8] (AB243875)

Immunohistochemical analysis of Formalin-Fixed Paraffin-Embedded mouse renal cell carcinoma labeling mouse SMC1 with ab243875, followed by a HRP-conjugated goat anti-rabbit IgG as secondary. Substrate : DAB. Nuclear staining on mouse renal cell carcinoma is observed. Counter stained with hematoxylin. Epitope retrieval with citrate buffer pH 6.0 for 20 minutes is recommended.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A antibody [BL-205-2G8] (AB243875)

Formalin-fixed, paraffin-embedded human Breast carcinoma tissue stained for SMC1A using ab243875 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A antibody [BL-205-2G8] (AB243875)

Immunohistochemical analysis of Formalin-Fixed Paraffin-Embedded human breast carcinoma tissue labeling human SMC1 (Red) with ab243875 followed by a HRP-conjugated goat anti-rabbit IgG as secondary. Substrate : Opal™. Nuclear staining on human breast carcinoma tissue is observed. Counter stained with DAPI (Blue). Epitope retrieval with citrate buffer pH 6.0 for 20 minutes is recommended.

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Immunocytochemistry - Anti-SMC1A antibody [BL-205-2G8] (AB243875)

Immunocytochemical analysis of Formalin-Fixed Paraffin-Embedded NCI-H226 cells labeling human SMC1 with ab243875 followed by a HRP-conjugated goat anti-rabbit IgG as secondary. Substrate : DAB. Nuclear staining on NCI-H226 cells is observed. Counter stained with hematoxylin. Epitope retrieval with citrate buffer pH 6.0 for 20 minutes is recommended.

Flow Cytometry - Anti-SMC1A antibody [BL-205-2G8] (AB243875)

Flow Cyt

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Flow Cytometry - Anti-SMC1A antibody [BL-205-2G8] (AB243875)

Flow cytometry overlay histogram of 4% formaldehyde fixed HT-29 cells permeabilized with 90% methanol labeling human SMC1 with ab243875 at 1 µl per 1 x 10^6 cells. (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

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Immunocytochemistry - Anti-SMC1A antibody [BL-205-2G8] (AB243875)

Formaldehyde-fixed HCT-116 (human colorectal carcinoma cell line) cells labeling SMC1A using ab243875 at 1/250 dilution in ICC analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody. DAB staining

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Immunoprecipitation - Anti-SMC1A antibody [BL-205-2G8] (AB243875)

SMC1A was immunoprecipitated from 1 mg HEK-293T whole cell lysate with ab243875 at 20 μl per reaction Western blot was performed on the immunoprecipitate using ab243875 at 1/1000 dilution.

Lane 1 : ab243875 IP in HEK-293T whole cell lysate.

Lane 2 : Contol IgG in HEK-293T whole cell lysate.

Detection : Chemiluminescence with an exposure time of 10 seconds.

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Immunoprecipitation - Anti-SMC1A antibody [BL-205-2G8] (ab243875)

Predicted band size: 143 kDa

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IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A antibody [BL-205-2G8] (AB243875)

Immunohistochemical analysis of Formalin-Fixed Paraffin-Embedded human ovarian carcinoma tissue labeling mouse SMC1 with ab243875, followed by a HRP-conjugated goat anti-rabbit IgG as secondary. Substrate : DAB. Nuclear staining on human ovarian carcinoma is observed. Counter stained with hematoxylin. Epitope retrieval with citrate buffer pH 6.0 for 20 minutes is recommended.

Flow Cyt

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Flow Cytometry - Anti-SMC1A antibody [BL-205-2G8] (AB243875)

Flow cytometry overlay histogram of 4% formaldehyde fixed NIH3T3 cells permeabilized with 90% methanol labeling human SMC1 with ab243875 at 1 µl per 1 x 10^6 cells etoposide treated (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

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Western blot - Anti-SMC1A antibody [BL-205-2G8] (AB243875)

Western blot analysis using ab243875 at 1/1000 dilution.

Lane 1 : HeLa whole cell lysate (15 µg).

Lane 2 : HEK-293T whole cell lysate (15 µg).

Lane3 : MCF7 whole cell lysate (15 µg).

Lane 4 : HepG2 whole cell lysate (15 µg).

Lane 5 : A549 whole cell lysate (15 µg).

Lane 6 : SW620 whole cell lysate (15 µg).

Lane 7 : SK whole cell lysate (15µg).

Lane 8 : Jurkat whole cell lysate (15 µg).

Lane 9 : TCMK-1 whole cell lysate (15 µg).

Lane 10 : NIH/3T3 whole cell lysate (15 µg).

Lane 11 : CT26 whole cell lysate (15 µg)

A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection : chemiluminescence with an exposure time of 10 seconds

All lanes:

Western blot - Anti-SMC1A antibody [BL-205-2G8] (ab243875)

Predicted band size: 143 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BL-205-2G8

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P, Flow Cyt, IP, ICC

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100 - 1/500", "ICC-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "Use 20μl/mg lysate.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µL for 10^6 Cells", "FlowCyt-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µL for 10^6 Cells", "FlowCyt-species-notes": "" } } }

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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

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Properties and storage information

Form

Liquid

Purification notes

Recombinant antibody was purified from cell culture supernatant.

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMC1A also known as structural maintenance of chromosomes 1A is an essential component of the cohesin complex. This complex regulates the cohesion of sister chromatids ensuring accurate chromosome segregation during cell division. With a molecular mass of around 143 kDa SMC1A is expressed ubiquitously with higher levels in proliferating cells. It is a member of the SMC protein family possessing distinct N-terminal and C-terminal domains connected by a flexible coiled-coil region.

Biological function summary

SMC1A forms a complex with other proteins most notably SMC3 RAD21 and STAG2. This complex is necessary for maintaining chromatid cohesion during the cell cycle's S and G2 phases. Furthermore SMC1A influences DNA repair specifically homologous recombination by maintaining genome stability. Through its associations SMC1A contributes to regulation of gene expression further illustrating its multifunctional role in cellular processes.

Pathways

SMC1A integrates into DNA damage response and repair pathways. The ATR and ATM signaling pathways interact with SMC1A modulating its function during DNA repair mechanisms. SMC1A associates with proteins like PDS5 and WAPL which influence cohesin dynamics. These interactions stress SMC1A’s role in preserving genomic integrity and controlling cell cycle progression.

Mutations or deregulation of SMC1A links to Cornelia de Lange Syndrome and certain cancers. This role is particularly significant in Cornelia de Lange Syndrome where SMC1A mutations disrupt normal chromosomal condensation and separation. Within oncogenesis SMC1A's interaction with TP53 indicates a broader influence on tumorigenesis emphasizing its involvement in maintaining cellular homeostasis and preventing cancer progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.

See full target information SMC1A

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112266 PubMed40241752

2025

CRISPR/Cas9-mediated SHP-1-knockout T cells combined with simvastatin enhances anti-tumor activity in humanized-PDX HCC model.

Applications

Unspecified application

Species

Unspecified reactive species

Huaping Liu,Wu Ge,Xiaoping Yu,Jianwei Luo,Juan Zhang,Min Yang,Lu Cao,Yangnan Zhang,Ruike Wang,Cejun Yang,Pei Li,Mengyu Tian,XiaoPei Peng,Lei Peng,Di Wu,Muqi Liu,Qi Liang,Shengwang Zhang,Wei Li,Pengfei Rong,Hailan Li,Xiaoqian Ma,Wei Wang

Molecular therapy. Nucleic acids 27:133-146 PubMed34976433

2022

SMC1A regulated by KIAA1429 in m6A-independent manner promotes EMT progress in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Zhang,Xin-Yuan Dai,Jia-Yi Qian,Feng Xu,Zhang-Wei Wang,Tian Xia,Xu-Jie Zhou,Xiao-Xia Li,Liang Shi,Ji-Fu Wei,Qiang Ding

Reproductive biology and endocrinology : RB&E 19:35 PubMed33653363

2021

Label-free proteomics uncovers SMC1A expression is Down-regulated in AUB-E.

Applications

Unspecified application

Species

Unspecified reactive species

Yingxian Jia,Jie Luo,Yibing Lan,Chunming Li,Linjuan Ma,Xiaoming Zhu,Fei Ruan,Jianhong Zhou

View all publications

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