AB75819

Anti-SMC1A antibody [EP2879Y]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal SMC1A antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications.

View Alternative Names

DXS423E, KIAA0178, SB1.8, SMC1, SMC1L1, SMC1A, Structural maintenance of chromosomes protein 1A, SMC protein 1A, SMC-1-alpha, SMC-1A, Sb1.8

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-SMC1A antibody [EP2879Y] (AB75819)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SMC1A antibody [EP2879Y] (AB75819)

ab75819 at 1/100 dilution staining SMC1A in HeLa cells by Immunofluorescence.

Unknown

Western blot - Anti-SMC1A antibody [EP2879Y] (AB75819)

All lanes:

Western blot - Anti-SMC1A antibody [EP2879Y] (ab75819) at 1/500 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

NIH3T3 cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 143 kDa

Observed band size: 143 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2879Y

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMC1A also known as structural maintenance of chromosomes 1A is an essential component of the cohesin complex. This complex regulates the cohesion of sister chromatids ensuring accurate chromosome segregation during cell division. With a molecular mass of around 143 kDa SMC1A is expressed ubiquitously with higher levels in proliferating cells. It is a member of the SMC protein family possessing distinct N-terminal and C-terminal domains connected by a flexible coiled-coil region.

Biological function summary

SMC1A forms a complex with other proteins most notably SMC3 RAD21 and STAG2. This complex is necessary for maintaining chromatid cohesion during the cell cycle's S and G2 phases. Furthermore SMC1A influences DNA repair specifically homologous recombination by maintaining genome stability. Through its associations SMC1A contributes to regulation of gene expression further illustrating its multifunctional role in cellular processes.

Pathways

SMC1A integrates into DNA damage response and repair pathways. The ATR and ATM signaling pathways interact with SMC1A modulating its function during DNA repair mechanisms. SMC1A associates with proteins like PDS5 and WAPL which influence cohesin dynamics. These interactions stress SMC1A’s role in preserving genomic integrity and controlling cell cycle progression.

Mutations or deregulation of SMC1A links to Cornelia de Lange Syndrome and certain cancers. This role is particularly significant in Cornelia de Lange Syndrome where SMC1A mutations disrupt normal chromosomal condensation and separation. Within oncogenesis SMC1A's interaction with TP53 indicates a broader influence on tumorigenesis emphasizing its involvement in maintaining cellular homeostasis and preventing cancer progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.

See full target information SMC1A

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature 623:183-192 PubMed37853125

2023

Chromatin compartmentalization regulates the response to DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Coline Arnould,Vincent Rocher,Florian Saur,Aldo S Bader,Fernando Muzzopappa,Sarah Collins,Emma Lesage,Benjamin Le Bozec,Nadine Puget,Thomas Clouaire,Thomas Mangeat,Raphael Mourad,Nadav Ahituv,Daan Noordermeer,Fabian Erdel,Martin Bushell,Aline Marnef,Gaëlle Legube

Cell cycle (Georgetown, Tex.) 16:536-544 PubMed28118058

2017

Smc1β is required for activation of SAC during mouse oocyte meiosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yilong Miao,Changyin Zhou,Zhaokang Cui,Xiaoxin Dai,Mianqun Zhang,Yajuan Lu,Bo Xiong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SMC1A antibody [EP2879Y]

Immunocytochemistry/ Immunofluorescence - Anti-SMC1A antibody [EP2879Y] (AB75819)

Western blot - Anti-SMC1A antibody [EP2879Y] (AB75819)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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