Rabbit Recombinant Monoclonal SMC1A antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human, Mouse samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-SMC1A antibody [EP2879Y] - BSA and Azide free (AB247404), expandable thumbnail](https://content.abcam.com/products/images/smc1a-antibody-ep2879y-bsa-and-azide-free-ab247404--immunocytochemistry-immunofluorescence-img140200.gif "Immunocytochemistry/ Immunofluorescence - Anti-SMC1A antibody [EP2879Y] - BSA and Azide free (AB247404)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Expected | Not recommended | Not recommended | Tested | Not recommended |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Target data
Function
Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.
Alternative names
DXS423E, KIAA0178, SB1.8, SMC1, SMC1L1, SMC1A, Structural maintenance of chromosomes protein 1A, SMC protein 1A, SMC-1-alpha, SMC-1A, Sb1.8
Recommended products
Rabbit Recombinant Monoclonal SMC1A antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP2879Y
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab247404 is the carrier-free version of Anti-SMC1A antibody [EP2879Y] ab75819.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SMC1A also known as structural maintenance of chromosomes 1A is an essential component of the cohesin complex. This complex regulates the cohesion of sister chromatids ensuring accurate chromosome segregation during cell division. With a molecular mass of around 143 kDa SMC1A is expressed ubiquitously with higher levels in proliferating cells. It is a member of the SMC protein family possessing distinct N-terminal and C-terminal domains connected by a flexible coiled-coil region.
- Biological function summary
SMC1A forms a complex with other proteins most notably SMC3 RAD21 and STAG2. This complex is necessary for maintaining chromatid cohesion during the cell cycle's S and G2 phases. Furthermore SMC1A influences DNA repair specifically homologous recombination by maintaining genome stability. Through its associations SMC1A contributes to regulation of gene expression further illustrating its multifunctional role in cellular processes.
- Pathways
SMC1A integrates into DNA damage response and repair pathways. The ATR and ATM signaling pathways interact with SMC1A modulating its function during DNA repair mechanisms. SMC1A associates with proteins like PDS5 and WAPL which influence cohesin dynamics. These interactions stress SMC1A’s role in preserving genomic integrity and controlling cell cycle progression.
- Associated diseases and disorders
Mutations or deregulation of SMC1A links to Cornelia de Lange Syndrome and certain cancers. This role is particularly significant in Cornelia de Lange Syndrome where SMC1A mutations disrupt normal chromosomal condensation and separation. Within oncogenesis SMC1A's interaction with TP53 indicates a broader influence on tumorigenesis emphasizing its involvement in maintaining cellular homeostasis and preventing cancer progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Immunocytochemistry/ Immunofluorescence - Anti-SMC1A antibody [EP2879Y] - BSA and Azide free (ab247404)
This data was developed using Anti-SMC1A antibody [EP2879Y] ab75819, the same antibody clone in a different buffer formulation.Anti-SMC1A antibody [EP2879Y] ab75819 at 1/100 dilution staining SMC1A in HeLa cells by Immunofluorescence.
![Western blot - Anti-SMC1A antibody [EP2879Y] - BSA and Azide free (ab247404), expandable thumbnail](https://content.abcam.com/products/images/smc1a-antibody-ep2879y-bsa-and-azide-free-ab247404--western-blot-img136852.gif "Western blot - Anti-SMC1A antibody [EP2879Y] - BSA and Azide free (ab247404)")
Western blot - Anti-SMC1A antibody [EP2879Y] - BSA and Azide free (ab247404)
This data was developed using Anti-SMC1A antibody [EP2879Y] ab75819, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-SMC1A antibody [EP2879Y] (Anti-SMC1A antibody [EP2879Y] ab75819) at 1/500 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: 293T cell lysate at 10 µg
Lane 3: NIH3T3 cell lysate at 10 µg
SecondaryAll lanes: goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 143 kDa
Observed band size: 143 kDa
Downloads
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