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AB1276

Anti-SMC1A (phospho S966) antibody

4

(2 Reviews)

|

(17 Publications)

Rabbit Polyclonal SMC1A phospho S966 antibody. Suitable for ICC, IP, WB and reacts with Human samples. Cited in 17 publications. Immunogen corresponding to Synthetic Peptide within Human SMC1A phospho S966 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

DXS423E, KIAA0178, SB1.8, SMC1, SMC1L1, SMC1A, Structural maintenance of chromosomes protein 1A, SMC protein 1A, SMC-1-alpha, SMC-1A, Sb1.8

4 Images
Immunocytochemistry - Anti-SMC1A (phospho S966) antibody (AB1276)
  • ICC

Unknown

Immunocytochemistry - Anti-SMC1A (phospho S966) antibody (AB1276)

Immunocytochemistry analysis of neocarzinostatin treated asynchronous HeLa cells labelling SMC1A (phospho S966) with ab1276 at 1/5000 (0.2μg/ml). A DyLight® 594-conjugated anti-rabbit IgG (1/100) was used as the secondary antibody.

Immunoprecipitation - Anti-SMC1A (phospho S966) antibody (AB1276)
  • IP

Supplier Data

Immunoprecipitation - Anti-SMC1A (phospho S966) antibody (AB1276)

SMC1A was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate treated with 100 μM etoposide (+) or mock treated (-) with ab1276 at 1 μg/ml. Western blot was performed from the immunoprecipitate using ab1276 at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-SMC1A (phospho S966) antibody (ab1276)

Predicted band size: 143 kDa

false

Exposure time: 3min

Immunocytochemistry - Anti-SMC1A (phospho S966) antibody (AB1276)
  • ICC

AbReview3502****

Immunocytochemistry - Anti-SMC1A (phospho S966) antibody (AB1276)

ICC of SKN cells cultured on coverslips were fixed in 4% paraformaldehyde and then stained with Rabbit polyclonal to SMC1A (phospho S966), ab1276 (green) at a working dilution of 1/200. The DNA stained with DAPI is shown in red. (100x magnification).

This image was submitted as part of a review by Kirk McManus, University of British Columbia

Western blot - Anti-SMC1A (phospho S966) antibody (AB1276)
  • WB

Supplier Data

Western blot - Anti-SMC1A (phospho S966) antibody (AB1276)

All lanes:

Western blot - Anti-SMC1A (phospho S966) antibody (ab1276) at 0.1 µg/mL

Lane 1:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate with 100 µM etoposide (+) at 50 µg

Lane 2:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate with 100 µM etoposide (-) at 50 µg

Predicted band size: 143 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP, ICC

applications

Immunogen

Synthetic Peptide within Human SMC1A phospho S966 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q14683

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/500 - 1/5000", "ICC-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>" }, "Orangutan": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antibodies that were not phospho-specific were removed by solid phase absorption.Antibodies specific for SMC1A pSer957 were affinity purified using the phosphopeptide immobilized onsolid support.
Storage buffer
pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMC1A also known as structural maintenance of chromosomes 1A is an essential component of the cohesin complex. This complex regulates the cohesion of sister chromatids ensuring accurate chromosome segregation during cell division. With a molecular mass of around 143 kDa SMC1A is expressed ubiquitously with higher levels in proliferating cells. It is a member of the SMC protein family possessing distinct N-terminal and C-terminal domains connected by a flexible coiled-coil region.
Biological function summary

SMC1A forms a complex with other proteins most notably SMC3 RAD21 and STAG2. This complex is necessary for maintaining chromatid cohesion during the cell cycle's S and G2 phases. Furthermore SMC1A influences DNA repair specifically homologous recombination by maintaining genome stability. Through its associations SMC1A contributes to regulation of gene expression further illustrating its multifunctional role in cellular processes.

Pathways

SMC1A integrates into DNA damage response and repair pathways. The ATR and ATM signaling pathways interact with SMC1A modulating its function during DNA repair mechanisms. SMC1A associates with proteins like PDS5 and WAPL which influence cohesin dynamics. These interactions stress SMC1A’s role in preserving genomic integrity and controlling cell cycle progression.

Mutations or deregulation of SMC1A links to Cornelia de Lange Syndrome and certain cancers. This role is particularly significant in Cornelia de Lange Syndrome where SMC1A mutations disrupt normal chromosomal condensation and separation. Within oncogenesis SMC1A's interaction with TP53 indicates a broader influence on tumorigenesis emphasizing its involvement in maintaining cellular homeostasis and preventing cancer progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.
See full target information SMC1A phospho S966
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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Science advances 7: PubMed33627431

2021

The deacetylation-phosphorylation regulation of SIRT2-SMC1A axis as a mechanism of antimitotic catastrophe in early tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Yi,Ying Zhang,Zhijun Wang,Zhuo Wang,Ziwei Li,Tingting Zhou,Hongde Xu,Jingwei Liu,Bo Jiang,Xiaoman Li,Liang Wang,Ning Bai,Qiqiang Guo,Yi Guan,Yanling Feng,Zhiyong Mao,Guangjian Fan,Shengping Zhang,Chuangui Wang,Longyue Cao,Brian P O'Rourke,Yang Wang,Yanmei Wu,Boquan Wu,Shilong You,Naijin Zhang,Junlin Guan,Xiaoyu Song,Yingxian Sun,Shi Wei,Liu Cao

Molecular cell 74:571-583.e8 PubMed30898438

2019

MDC1 Interacts with TOPBP1 to Maintain Chromosomal Stability during Mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Pia-Amata Leimbacher,Samuel E Jones,Ann-Marie K Shorrocks,Mara de Marco Zompit,Matthew Day,Jordy Blaauwendraad,Diana Bundschuh,Sarah Bonham,Roman Fischer,Daniel Fink,Benedikt M Kessler,Antony W Oliver,Laurence H Pearl,Andrew N Blackford,Manuel Stucki

Molecular medicine reports 18:2599-2612 PubMed30015887

2018

Quercetin‑3‑O‑β‑D‑glucoside decreases the bioavailability of cyclosporin A through regulation of drug metabolizing enzymes, transporters and nuclear receptors in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Tingyu Yang,Yani Liu,Xixi Huang,Rui Zhang,Chunxiao Yang,Jiali Zhou,Yu Zhang,Jing Wan,Shaojun Shi

International journal of biological sciences 14:1081-1089 PubMed29988990

2018

Phosphorylation of SMC1A promotes hepatocellular carcinoma cell proliferation and migration.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Zhang,Fei Yi,Liang Wang,Zhuo Wang,Naijin Zhang,Zhijun Wang,Ziwei Li,Xiaoyu Song,Shi Wei,Liu Cao

Blood cancer journal 6:e465 PubMed27588518

2016

ATM function and its relationship with ATM gene mutations in chronic lymphocytic leukemia with the recurrent deletion (11q22.3-23.2).

Applications

Unspecified application

Species

Unspecified reactive species

Y Jiang,H-C Chen,X Su,P A Thompson,X Liu,K-A Do,W Wierda,M J Keating,W Plunkett

Cell cycle (Georgetown, Tex.) 13:2901-12 PubMed25486478

2014

Bimodal regulation of p21(waf1) protein as function of DNA damage levels.

Applications

WB

Species

Unspecified reactive species

G Buscemi,C Ricci,L Zannini,E Fontanella,P Plevani,D Delia

European journal of human genetics : EJHG 21:774-8 PubMed23211698

2012

Deep-intronic ATM mutation detected by genomic resequencing and corrected in vitro by antisense morpholino oligonucleotide (AMO).

Applications

Unspecified application

Species

Unspecified reactive species

Simona Cavalieri,Elisa Pozzi,Richard A Gatti,Alfredo Brusco

Biochemical pharmacology 81:222-32 PubMed20933509

2010

The synergistic cytotoxicity of clofarabine, fludarabine and busulfan in AML cells involves ATM pathway activation and chromatin remodeling.

Applications

WB

Species

Human

Benigno C Valdez,Yang Li,David Murray,Richard E Champlin,Borje S Andersson

Nature 467:343-6 PubMed20818375

2010

Phosphorylation of MLL by ATR is required for execution of mammalian S-phase checkpoint.

Applications

WB

Species

Human

Han Liu,Shugaku Takeda,Rakesh Kumar,Todd D Westergard,Eric J Brown,Tej K Pandita,Emily H-Y Cheng,James J-D Hsieh

The Journal of cell biology 190:731-40 PubMed20805324

2010

The chromatin-remodeling factor CHD4 coordinates signaling and repair after DNA damage.

Applications

WB

Species

Human

Dorthe Helena Larsen,Catherine Poinsignon,Thorkell Gudjonsson,Christoffel Dinant,Mark R Payne,Flurina J Hari,Jannie M Rendtlew Danielsen,Patrice Menard,Jette Christensen Sand,Manuel Stucki,Claudia Lukas,Jiri Bartek,Jens S Andersen,Jiri Lukas
View all publications
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Product promise

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