Rabbit Polyclonal SMC1A phospho S966 antibody. Suitable for ICC, IP, WB and reacts with Human samples. Cited in 17 publications. Immunogen corresponding to Synthetic Peptide within Human SMC1A phospho S966 conjugated to Keyhole Limpet Haemocyanin.
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- Science advances 7:2021The deacetylation-phosphorylation regulation of SIRT2-SMC1A axis as a mechanism of antimitotic catastrophe in early tumorigenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFei Yi,Ying Zhang,Zhijun Wang,Zhuo Wang,Ziwei Li,Tingting Zhou,Hongde Xu,Jingwei Liu,Bo Jiang,Xiaoman Li,Liang Wang,Ning Bai,Qiqiang Guo,Yi Guan,Yanling Feng,Zhiyong Mao,Guangjian Fan,Shengping Zhang,Chuangui Wang,Longyue Cao,Brian P O'Rourke,Yang Wang,Yanmei Wu,Boquan Wu,Shilong You,Naijin Zhang,Junlin Guan,Xiaoyu Song,Yingxian Sun,Shi Wei,Liu CaoPubMed 33627431
- Molecular cell 74:571-583.e82019MDC1 Interacts with TOPBP1 to Maintain Chromosomal Stability during Mitosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPia-Amata Leimbacher et. al.PubMed 30898438
- Molecular medicine reports 18:2599-26122018Quercetin‑3‑O‑β‑D‑glucoside decreases the bioavailability of cyclosporin A through regulation of drug metabolizing enzymes, transporters and nuclear receptors in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTingyu Yang et. al.PubMed 30015887
- International journal of biological sciences 14:1081-10892018Phosphorylation of SMC1A promotes hepatocellular carcinoma cell proliferation and migration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Zhang et. al.PubMed 29988990
- Blood cancer journal 6:e4652016ATM function and its relationship with ATM gene mutations in chronic lymphocytic leukemia with the recurrent deletion (11q22.3-23.2).Applications:Unspecified applicationReactive species:Unspecified reactive speciesY Jiang et. al.PubMed 27588518
- Cell cycle (Georgetown, Tex.) 13:2901-122014Bimodal regulation of p21(waf1) protein as function of DNA damage levels.Applications:WBReactive species:Unspecified reactive speciesG Buscemi,C Ricci,L Zannini,E Fontanella,P Plevani,D DeliaPubMed 25486478
- European journal of human genetics : EJHG 21:774-82012Deep-intronic ATM mutation detected by genomic resequencing and corrected in vitro by antisense morpholino oligonucleotide (AMO).Applications:Unspecified applicationReactive species:Unspecified reactive speciesSimona Cavalieri,Elisa Pozzi,Richard A Gatti,Alfredo BruscoPubMed 23211698
- Biochemical pharmacology 81:222-322010The synergistic cytotoxicity of clofarabine, fludarabine and busulfan in AML cells involves ATM pathway activation and chromatin remodeling.Applications:WBReactive species:HumanBenigno C Valdez,Yang Li,David Murray,Richard E Champlin,Borje S AnderssonPubMed 20933509
- Nature 467:343-62010Phosphorylation of MLL by ATR is required for execution of mammalian S-phase checkpoint.
- The Journal of cell biology 190:731-402010The chromatin-remodeling factor CHD4 coordinates signaling and repair after DNA damage.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SMC1A phospho S966 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q14683
Reactivity data
Select an application| ICC | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Orangutan | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rhesus monkey, Orangutan | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rhesus monkey, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rhesus monkey, Orangutan | Dilution info - | Notes - |
Associated Products
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Target data
Function
Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.
Alternative names
DXS423E, KIAA0178, SB1.8, SMC1, SMC1L1, SMC1A, Structural maintenance of chromosomes protein 1A, SMC protein 1A, SMC-1-alpha, SMC-1A, Sb1.8
Recommended products
Rabbit Polyclonal SMC1A phospho S966 antibody. Suitable for ICC, IP, WB and reacts with Human samples. Cited in 17 publications. Immunogen corresponding to Synthetic Peptide within Human SMC1A phospho S966 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SMC1A phospho S966 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q14683
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Antibodies that were not phospho-specific were removed by solid phase absorption.Antibodies specific for SMC1A pSer957 were affinity purified using the phosphopeptide immobilized onsolid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SMC1A also known as structural maintenance of chromosomes 1A is an essential component of the cohesin complex. This complex regulates the cohesion of sister chromatids ensuring accurate chromosome segregation during cell division. With a molecular mass of around 143 kDa SMC1A is expressed ubiquitously with higher levels in proliferating cells. It is a member of the SMC protein family possessing distinct N-terminal and C-terminal domains connected by a flexible coiled-coil region.
- Biological function summary
SMC1A forms a complex with other proteins most notably SMC3 RAD21 and STAG2. This complex is necessary for maintaining chromatid cohesion during the cell cycle's S and G2 phases. Furthermore SMC1A influences DNA repair specifically homologous recombination by maintaining genome stability. Through its associations SMC1A contributes to regulation of gene expression further illustrating its multifunctional role in cellular processes.
- Pathways
SMC1A integrates into DNA damage response and repair pathways. The ATR and ATM signaling pathways interact with SMC1A modulating its function during DNA repair mechanisms. SMC1A associates with proteins like PDS5 and WAPL which influence cohesin dynamics. These interactions stress SMC1A’s role in preserving genomic integrity and controlling cell cycle progression.
- Associated diseases and disorders
Mutations or deregulation of SMC1A links to Cornelia de Lange Syndrome and certain cancers. This role is particularly significant in Cornelia de Lange Syndrome where SMC1A mutations disrupt normal chromosomal condensation and separation. Within oncogenesis SMC1A's interaction with TP53 indicates a broader influence on tumorigenesis emphasizing its involvement in maintaining cellular homeostasis and preventing cancer progression.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Immunoprecipitation - Anti-SMC1A (phospho S966) antibody (ab1276)
SMC1A was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate treated with 100 μM etoposide (+) or mock treated (-) with ab1276 at 1 μg/ml. Western blot was performed from the immunoprecipitate using ab1276 at 1/1000 dilution.
All lanes: Immunoprecipitation - Anti-SMC1A (phospho S966) antibody (ab1276)
Predicted band size: 143 kDa
Exposure time: 3min
Western blot - Anti-SMC1A (phospho S966) antibody (ab1276)
All lanes: Western blot - Anti-SMC1A (phospho S966) antibody (ab1276) at 0.1 µg/mL
Lane 1: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate with 100 µM etoposide (+) at 50 µg
Lane 2: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate with 100 µM etoposide (-) at 50 µg
Predicted band size: 143 kDa
Exposure time: 3min
Immunocytochemistry - Anti-SMC1A (phospho S966) antibody (ab1276)
Immunocytochemistry analysis of neocarzinostatin treated asynchronous HeLa cells labelling SMC1A (phospho S966) with ab1276 at 1/5000 (0.2μg/ml). A DyLight® 594-conjugated anti-rabbit IgG (1/100) was used as the secondary antibody.
This image was submitted as part of a review by Kirk McManus, University of British ColumbiaImmunocytochemistry - Anti-SMC1A (phospho S966) antibody (ab1276)
ICC of SKN cells cultured on coverslips were fixed in 4% paraformaldehyde and then stained with Rabbit polyclonal to SMC1A (phospho S966), ab1276 (green) at a working dilution of 1/200. The DNA stained with DAPI is shown in red. (100x magnification).
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