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AB284774

Anti-SMC1A (phospho S966) antibody [EP2858Y] - BSA and Azide free

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Rabbit Monoclonal SMC1A phospho S966 antibody. Carrier free. Suitable for ICC/IF, WB, Dot and reacts with Human, Synthetic peptide samples.

View Alternative Names

DXS423E, KIAA0178, SB1.8, SMC1, SMC1L1, SMC1A, Structural maintenance of chromosomes protein 1A, SMC protein 1A, SMC-1-alpha, SMC-1A, Sb1.8

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-SMC1A (phospho S966) antibody [EP2858Y] - BSA and Azide free (AB284774)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SMC1A (phospho S966) antibody [EP2858Y] - BSA and Azide free (AB284774)

This data was developed using ab81306, the same antibody clone in a different buffer.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling SMC1A with ab81306 at 1/50 (2 µg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) at 1/1000 dilution. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889) was used to counterstain tubulin at 1/200 dilution. Confocal image showing nuclear staining in HeLa cells treated with hydroxyurea (3mM) for 20h. The nuclear counterstain was DAPI (Blue).

Western blot - Anti-SMC1A (phospho S966) antibody [EP2858Y] - BSA and Azide free (AB284774)
  • WB

Supplier Data

Western blot - Anti-SMC1A (phospho S966) antibody [EP2858Y] - BSA and Azide free (AB284774)

This data was developed using ab81306, the same antibody clone in a different buffer.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SMC1A (phospho S966) antibody [EP2858Y] (<a href='/en-us/products/primary-antibodies/smc1a-phospho-s966-antibody-ep2858y-ab81306'>ab81306</a>) at 1/5000 dilution

Lane 1:

Untreated HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 15 µg

Lane 2:

HEK-293 treated with 5mM Hydroxyurea for 18h whole cell lysate at 15 µg

Lane 3:

HEK-293 treated with 5mM Hydroxyurea for 18h whole cell lysate, then the membrane was incubated with phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 160 kDa

Observed band size: 160 kDa

false

Exposure time: 40s

Dot Blot - Anti-SMC1A (phospho S966) antibody [EP2858Y] - BSA and Azide free (AB284774)
  • Dot

Supplier Data

Dot Blot - Anti-SMC1A (phospho S966) antibody [EP2858Y] - BSA and Azide free (AB284774)

This data was developed using ab81306, the same antibody clone in a different buffer.

Dot blot analysis of SMC1A (phospho S966) using ab76584 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated (ab97051) at 1/100,000 dilution. Blocking and dilution buffer : 5% NFDM/TBST. Exposure time : 180s.

All lanes:

Dot Blot - Anti-SMC1A (phospho S966) antibody [EP2858Y] (<a href='/en-us/products/primary-antibodies/smc1a-phospho-s966-antibody-ep2858y-ab81306'>ab81306</a>) at 1/1000 dilution

Lane 1:

SMC1A (phospho S966) peptide

Lane 2:

SMC1A non-phospho peptide

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2858Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Synthetic peptide": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>" } } }
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Product details

ab284774 is the carrier-free version of ab81306.

This product has switched from a hybridoma to recombinant production method on 23rd February 2024.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMC1A also known as structural maintenance of chromosomes 1A is an essential component of the cohesin complex. This complex regulates the cohesion of sister chromatids ensuring accurate chromosome segregation during cell division. With a molecular mass of around 143 kDa SMC1A is expressed ubiquitously with higher levels in proliferating cells. It is a member of the SMC protein family possessing distinct N-terminal and C-terminal domains connected by a flexible coiled-coil region.
Biological function summary

SMC1A forms a complex with other proteins most notably SMC3 RAD21 and STAG2. This complex is necessary for maintaining chromatid cohesion during the cell cycle's S and G2 phases. Furthermore SMC1A influences DNA repair specifically homologous recombination by maintaining genome stability. Through its associations SMC1A contributes to regulation of gene expression further illustrating its multifunctional role in cellular processes.

Pathways

SMC1A integrates into DNA damage response and repair pathways. The ATR and ATM signaling pathways interact with SMC1A modulating its function during DNA repair mechanisms. SMC1A associates with proteins like PDS5 and WAPL which influence cohesin dynamics. These interactions stress SMC1A’s role in preserving genomic integrity and controlling cell cycle progression.

Mutations or deregulation of SMC1A links to Cornelia de Lange Syndrome and certain cancers. This role is particularly significant in Cornelia de Lange Syndrome where SMC1A mutations disrupt normal chromosomal condensation and separation. Within oncogenesis SMC1A's interaction with TP53 indicates a broader influence on tumorigenesis emphasizing its involvement in maintaining cellular homeostasis and preventing cancer progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.
See full target information SMC1A phospho S966
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Product promise

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For full details, please see our Terms & Conditions

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