AB10399

Anti-SMC2 antibody

Name: Anti-SMC2 antibody (ab10399) | Abcam
Brand: Abcam Limited
SKU: AB10399
Rating: 4 (3 reviews)

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What is this?

(3 Reviews)

(9 Publications)

Rabbit Polyclonal SMC2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human SMC2 aa 50-100.

View Alternative Names

CAPE, SMC2L1, PRO0324, SMC2, Structural maintenance of chromosomes protein 2, SMC protein 2, SMC-2, Chromosome-associated protein E, XCAP-E homolog, hCAP-E

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC2 antibody (AB10399)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC2 antibody (AB10399)

IHC image of ab10399 staining in normal human cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10399, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Supplier Data

Western blot - Anti-SMC2 antibody (AB10399)

All lanes:

Western blot - Anti-SMC2 antibody (ab10399) at 0.1 µg/mL

Lane 1:

E: Mock transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 90 µg

Lane 2:

T: SMC2 transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 90 µg

Predicted band size: 135 kDa

false

Exposure time: 10min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human SMC2 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

O95347

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Ferret": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antibodies were affinity purified using the peptide immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMC2 also known as Structural Maintenance of Chromosomes Protein 2 is a critical component of the Condensin complex with a mass of approximately 135 kDa. This protein primarily functions within the nucleus of the cell where it plays an important role in chromosome condensation during cell division. SMC2 belongs to the SMC family of proteins and is widely expressed in various tissues including highly proliferative ones like bone marrow and thymus. The protein structure includes coiled-coil and hinge regions that are vital for its function in maintaining chromosome structure.

Biological function summary

SMC2 plays an essential role in ensuring proper chromosome segregation during mitosis. It acts as part of the Condensin complex a multi-protein assembly that is also composed of SMC4 CAP-D2 and CAP-G among others. This complex is important for the restructuring of chromosomes into compact forms allowing them to be easily separated during cell division. SMC2 binds to DNA and aids in the resolution of sister chromatids facilitating the equal distribution of genetic material to daughter cells.

Pathways

SMC2 operates within the cell cycle control pathway where it is involved in mitotic processes. It interacts directly with other components of the cell cycle machinery including Cyclin-dependent kinase 1 (CDK1). SMC2 also plays a role in the DNA repair pathway partnering with proteins like ATM (Ataxia-Telangiectasia Mutated kinase) which senses DNA damage and coordinates repair processes. These pathways highlight the interconnected nature of SMC2's functions in maintaining genetic stability.

SMC2 has been linked to certain cancers due to its pivotal role in ensuring proper mitosis. Disruptions in its function can lead to aneuploidy and genomic instability common features of tumorigenesis. Furthermore SMC2's involvement in DNA repair pathways implies a connection to disorders related to DNA damage like Ataxia-Telangiectasia. This disorder is marked by increased sensitivity to DNA damage and proteins like ATM are key partners of SMC2 in these repair processes. Understanding these links provides insights into potential therapeutic targets for addressing such diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.

See full target information SMC2

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Discover oncology 16:1835 PubMed41060497

2025

Development of a novel 4-protein signature for prognostic prediction in hepatitis B virus-induced hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

HaiFeng Xie,Yuhong Zhao,Xianzhong Luo,Heran Zhou,Pengcheng Tong

The Journal of cell biology 219: PubMed31881080

2019

Aurora A promotes chromosome congression by activating the condensin-dependent pool of KIF4A.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Poser,Renaud Caous,Ulrike Gruneberg,Francis A Barr

Cancers 11: PubMed31357676

2019

Diminished Condensin Gene Expression Drives Chromosome Instability That May Contribute to Colorectal Cancer Pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Allison K Baergen,Lucile M Jeusset,Zelda Lichtensztejn,Kirk J McManus

Biomedicine & pharmacotherapy = Biomedecine & phar 104:788-797 PubMed29852353

2018

Hydrogen gas inhibits lung cancer progression through targeting SMC3.

Applications

Species

Unspecified reactive species

Dongchang Wang,Lifei Wang,Yu Zhang,Yunxia Zhao,Gang Chen

Scientific reports 8:5430 PubMed29615716

2018

A multi-step transcriptional cascade underlies vascular regeneration in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Aditya S Shirali,Milagros C Romay,Austin I McDonald,Trent Su,Michelle E Steel,M Luisa Iruela-Arispe

Genes & development 29:1683-95 PubMed26302788

2015

A structural hierarchy mediated by multiple nuclear factors establishes IgH locus conformation.

Applications

ChIP

Species

Mouse

Tatiana Gerasimova,Changying Guo,Amalendu Ghosh,Xiang Qiu,Lindsey Montefiori,Jiyoti Verma-Gaur,Nancy M Choi,Ann J Feeney,Ranjan Sen

The EMBO journal 32:1168-82 PubMed23531880

2013

YY1 controls Igκ repertoire and B-cell development, and localizes with condensin on the Igκ locus.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Pan,Madhusudhan Papasani,Yi Hao,Marco Calamito,Fang Wei,William J Quinn Iii,Arindam Basu,Junwen Wang,Suchita Hodawadekar,Kristina Zaprazna,Huifei Liu,Yang Shi,David Allman,Michael Cancro,Michael L Atchison

PloS one 7:e36045 PubMed22540018

2012

Structure of metaphase chromosomes: a role for effects of macromolecular crowding.

Applications

ICC/IF

Species

Unspecified reactive species

Ronald Hancock

The Journal of cell biology 188:491-503 PubMed20176923

2010

Condensin complexes regulate mitotic progression and interphase chromatin structure in embryonic stem cells.

Applications

Species

Mouse

Thomas G Fazzio,Barbara Panning

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SMC2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC2 antibody (AB10399)

Western blot - Anti-SMC2 antibody (AB10399)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Product promise