Rabbit Polyclonal SMC2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human SMC2 aa 50-100.
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Publications
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- The Journal of cell biology 219:2019Aurora A promotes chromosome congression by activating the condensin-dependent pool of KIF4A.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElena Poser,Renaud Caous,Ulrike Gruneberg,Francis A BarrPubMed 31881080
- Cancers 11:2019Diminished Condensin Gene Expression Drives Chromosome Instability That May Contribute to Colorectal Cancer Pathogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAllison K Baergen et. al.PubMed 31357676
- Biomedicine & pharmacotherapy = Biomedecine & phar 104:788-7972018Hydrogen gas inhibits lung cancer progression through targeting SMC3.
- Scientific reports 8:54302018A multi-step transcriptional cascade underlies vascular regeneration in vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAditya S Shirali et. al.PubMed 29615716
- Genes & development 29:1683-952015A structural hierarchy mediated by multiple nuclear factors establishes IgH locus conformation.Applications:ChIPReactive species:MouseTatiana Gerasimova,Changying Guo,Amalendu Ghosh,Xiang Qiu,Lindsey Montefiori,Jiyoti Verma-Gaur,Nancy M Choi,Ann J Feeney,Ranjan SenPubMed 26302788
- The EMBO journal 32:1168-822013YY1 controls Igκ repertoire and B-cell development, and localizes with condensin on the Igκ locus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuan Pan et. al.PubMed 23531880
- PloS one 7:e360452012Structure of metaphase chromosomes: a role for effects of macromolecular crowding.
- The Journal of cell biology 188:491-5032010Condensin complexes regulate mitotic progression and interphase chromatin structure in embryonic stem cells.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SMC2 aa 50-100. The exact immunogen used to generate this antibody is proprietary information. Database link O95347
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Chimpanzee | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Ferret | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Guinea pig | Predicted | Predicted |
| Horse | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000.00000 - 1/20000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan | Dilution info - | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.
Alternative names
CAPE, SMC2L1, PRO0324, SMC2, Structural maintenance of chromosomes protein 2, SMC protein 2, SMC-2, Chromosome-associated protein E, XCAP-E homolog, hCAP-E
Recommended products
Rabbit Polyclonal SMC2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human SMC2 aa 50-100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SMC2 aa 50-100. The exact immunogen used to generate this antibody is proprietary information. Database link O95347
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SMC2 also known as Structural Maintenance of Chromosomes Protein 2 is a critical component of the Condensin complex with a mass of approximately 135 kDa. This protein primarily functions within the nucleus of the cell where it plays an important role in chromosome condensation during cell division. SMC2 belongs to the SMC family of proteins and is widely expressed in various tissues including highly proliferative ones like bone marrow and thymus. The protein structure includes coiled-coil and hinge regions that are vital for its function in maintaining chromosome structure.
- Biological function summary
SMC2 plays an essential role in ensuring proper chromosome segregation during mitosis. It acts as part of the Condensin complex a multi-protein assembly that is also composed of SMC4 CAP-D2 and CAP-G among others. This complex is important for the restructuring of chromosomes into compact forms allowing them to be easily separated during cell division. SMC2 binds to DNA and aids in the resolution of sister chromatids facilitating the equal distribution of genetic material to daughter cells.
- Pathways
SMC2 operates within the cell cycle control pathway where it is involved in mitotic processes. It interacts directly with other components of the cell cycle machinery including Cyclin-dependent kinase 1 (CDK1). SMC2 also plays a role in the DNA repair pathway partnering with proteins like ATM (Ataxia-Telangiectasia Mutated kinase) which senses DNA damage and coordinates repair processes. These pathways highlight the interconnected nature of SMC2's functions in maintaining genetic stability.
- Associated diseases and disorders
SMC2 has been linked to certain cancers due to its pivotal role in ensuring proper mitosis. Disruptions in its function can lead to aneuploidy and genomic instability common features of tumorigenesis. Furthermore SMC2's involvement in DNA repair pathways implies a connection to disorders related to DNA damage like Ataxia-Telangiectasia. This disorder is marked by increased sensitivity to DNA damage and proteins like ATM are key partners of SMC2 in these repair processes. Understanding these links provides insights into potential therapeutic targets for addressing such diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-SMC2 antibody (ab10399)
All lanes: Western blot - Anti-SMC2 antibody (ab10399) at 0.1 µg/mL
Lane 1: E: Mock transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 90 µg
Lane 2: T: SMC2 transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 90 µg
Predicted band size: 135 kDa
Exposure time: 10min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC2 antibody (ab10399)
IHC image of ab10399 staining in normal human cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10399, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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