AB10412

Anti-SMC2 antibody

Name: Anti-SMC2 antibody (ab10412) | Abcam
Brand: Abcam Limited
SKU: AB10412
Rating: 4 (1 reviews)

(1 Review)

(26 Publications)

Rabbit Polyclonal SMC2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 26 publications. Immunogen corresponding to Synthetic Peptide within Human SMC2.

View Alternative Names

CAPE, SMC2L1, PRO0324, SMC2, Structural maintenance of chromosomes protein 2, SMC protein 2, SMC-2, Chromosome-associated protein E, XCAP-E homolog, hCAP-E

2 Images

Supplier Data

Immunoprecipitation - Anti-SMC2 antibody (AB10412)

Immunoprecipitation of Human Aurora A with ab10412 at 1 μg/mL.

Lane 1 : ab10412 + Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.

Lane 2 : IgG control + Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.

Detection : Chemiluminescence

All lanes:

Immunoprecipitation - Anti-SMC2 antibody (ab10412)

Predicted band size: 135 kDa

false

Exposure time: 3s

Supplier Data

Western blot - Anti-SMC2 antibody (AB10412)

All lanes:

Western blot - Anti-SMC2 antibody (ab10412) at 0.1 µg/mL

Lane 1:

HeLa cell lysate at 50 µg

Lane 2:

HEK293 cell lysate at 50 µg

Predicted band size: 135 kDa

false

Exposure time: 3s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human SMC2. The exact immunogen used to generate this antibody is proprietary information.

O95347

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1 µg/mL", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1 µg/mL", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antibodies were affinity purified using the peptide immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMC2 also known as Structural Maintenance of Chromosomes Protein 2 is a critical component of the Condensin complex with a mass of approximately 135 kDa. This protein primarily functions within the nucleus of the cell where it plays an important role in chromosome condensation during cell division. SMC2 belongs to the SMC family of proteins and is widely expressed in various tissues including highly proliferative ones like bone marrow and thymus. The protein structure includes coiled-coil and hinge regions that are vital for its function in maintaining chromosome structure.

Biological function summary

SMC2 plays an essential role in ensuring proper chromosome segregation during mitosis. It acts as part of the Condensin complex a multi-protein assembly that is also composed of SMC4 CAP-D2 and CAP-G among others. This complex is important for the restructuring of chromosomes into compact forms allowing them to be easily separated during cell division. SMC2 binds to DNA and aids in the resolution of sister chromatids facilitating the equal distribution of genetic material to daughter cells.

Pathways

SMC2 operates within the cell cycle control pathway where it is involved in mitotic processes. It interacts directly with other components of the cell cycle machinery including Cyclin-dependent kinase 1 (CDK1). SMC2 also plays a role in the DNA repair pathway partnering with proteins like ATM (Ataxia-Telangiectasia Mutated kinase) which senses DNA damage and coordinates repair processes. These pathways highlight the interconnected nature of SMC2's functions in maintaining genetic stability.

SMC2 has been linked to certain cancers due to its pivotal role in ensuring proper mitosis. Disruptions in its function can lead to aneuploidy and genomic instability common features of tumorigenesis. Furthermore SMC2's involvement in DNA repair pathways implies a connection to disorders related to DNA damage like Ataxia-Telangiectasia. This disorder is marked by increased sensitivity to DNA damage and proteins like ATM are key partners of SMC2 in these repair processes. Understanding these links provides insights into potential therapeutic targets for addressing such diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.

See full target information SMC2

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Publications (26)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:492 PubMed40133469

2025

Condensin II interacts with BLM helicase in S phase to maintain genome stability.

Applications

Unspecified application

Species

Unspecified reactive species

Brian Rodemoyer,Ganesha Kariyawasam,Veena Subramanian,Kristina Schmidt

Nature communications 16:1675 PubMed39955279

2025

SRBD1 facilitates chromosome segregation by promoting topoisomerase IIα localization to mitotic chromosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Courtney A Lovejoy,Sarah R Wessel,Rahul Bhowmick,Yuki Hatoyama,Masato T Kanemaki,Runxiang Zhao,David Cortez

The Journal of cell biology 224: PubMed39786339

2025

Quantitative imaging of loop extruders rebuilding interphase genome architecture after mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Brunner,Natalia Rosalía Morero,Wanlu Zhang,M Julius Hossain,Marko Lampe,Hannah Pflaumer,Aliaksandr Halavatyi,Jan-Michael Peters,Kai S Beckwith,Jan Ellenberg

Nature communications 15:7152 PubMed39169041

2024

Single-nucleosome imaging unveils that condensins and nucleosome-nucleosome interactions differentially constrain chromatin to organize mitotic chromosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Kayo Hibino,Yuji Sakai,Sachiko Tamura,Masatoshi Takagi,Katsuhiko Minami,Toyoaki Natsume,Masa A Shimazoe,Masato T Kanemaki,Naoko Imamoto,Kazuhiro Maeshima

Genes & development 34:1619-1636 PubMed33122293

2020

Replication stress conferred by POT1 dysfunction promotes telomere relocalization to the nuclear pore.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra M Pinzaru,Mike Kareh,Noa Lamm,Eros Lazzerini-Denchi,Anthony J Cesare,Agnel Sfeir

Cell reports 31:107533 PubMed32320646

2020

Inducible Degradation of the Human SMC5/6 Complex Reveals an Essential Role Only during Interphase.

Applications

Unspecified application

Species

Unspecified reactive species

Andrés Bueno Venegas,Toyoaki Natsume,Masato Kanemaki,Ian D Hickson

Nucleus (Austin, Tex.) 11:1-18 PubMed31924112

2020

Hyperosmotic stress: chromatin phase separation.

Applications

Unspecified application

Species

Unspecified reactive species

Ada L Olins,Travis J Gould,Logan Boyd,Bettina Sarg,Donald E Olins

Journal of cell science 132: PubMed31653782

2019

Condensin II protein dysfunction impacts mitochondrial respiration and mitochondrial oxidative stress responses.

Applications

Unspecified application

Species

Unspecified reactive species

Emily Deutschman,Jacqueline R Ward,Avinash Kumar,Greeshma Ray,Nicole Welch,Madeleine E Lemieux,Srinivisan Dasarathy,Michelle S Longworth

Nature cell biology 21:1393-1402 PubMed31685986

2019

A chromosome folding intermediate at the condensin-to-cohesin transition during telophase.

Applications

Unspecified application

Species

Unspecified reactive species

Kristin Abramo,Anne-Laure Valton,Sergey V Venev,Hakan Ozadam,A Nicole Fox,Job Dekker

The Journal of cell biology 218:1531-1552 PubMed30858191

2019

Live imaging of marked chromosome regions reveals their dynamic resolution and compaction in mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

John K Eykelenboom,Marek Gierliński,Zuojun Yue,Nadia Hegarat,Hilary Pollard,Tatsuo Fukagawa,Helfrid Hochegger,Tomoyuki U Tanaka

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-SMC2 antibody

Immunoprecipitation - Anti-SMC2 antibody (AB10412)

Western blot - Anti-SMC2 antibody (AB10412)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Complementary Products

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (26)

Product promise