JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB229353

Anti-SMC2 antibody

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Polyclonal SMC2 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SMC2.

View Alternative Names

CAPE, SMC2L1, PRO0324, SMC2, Structural maintenance of chromosomes protein 2, SMC protein 2, SMC-2, Chromosome-associated protein E, XCAP-E homolog, hCAP-E

2 Images
Western blot - Anti-SMC2 antibody (AB229353)
  • WB

Supplier Data

Western blot - Anti-SMC2 antibody (AB229353)

5% SDS-PAGE

All lanes:

Western blot - Anti-SMC2 antibody (ab229353) at 1/1000 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts at 30 µg

Lane 2:

A431 (Human epidermoid carcinoma cell line) whole cell extracts at 30 µg

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extracts at 30 µg

Lane 4:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell extracts at 30 µg

Predicted band size: 135 kDa

true

Immunoprecipitation - Anti-SMC2 antibody (AB229353)
  • IP

Supplier Data

Immunoprecipitation - Anti-SMC2 antibody (AB229353)

SMC2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5 μg ab229353. Western blot was performed from the immunoprecipitate using ab229353.

Lane 1 : HeLa whole cell extract.

Lane 2 : Control IgG IP in HeLa whole cell extract.

Lane 3 : ab229353 IP in HeLa whole cell extract.

All lanes:

Immunoprecipitation - Anti-SMC2 antibody (ab229353)

Predicted band size: 135 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Recombinant Fragment Protein within Human SMC2. The exact immunogen used to generate this antibody is proprietary information.

O95347

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMC2 also known as Structural Maintenance of Chromosomes Protein 2 is a critical component of the Condensin complex with a mass of approximately 135 kDa. This protein primarily functions within the nucleus of the cell where it plays an important role in chromosome condensation during cell division. SMC2 belongs to the SMC family of proteins and is widely expressed in various tissues including highly proliferative ones like bone marrow and thymus. The protein structure includes coiled-coil and hinge regions that are vital for its function in maintaining chromosome structure.
Biological function summary

SMC2 plays an essential role in ensuring proper chromosome segregation during mitosis. It acts as part of the Condensin complex a multi-protein assembly that is also composed of SMC4 CAP-D2 and CAP-G among others. This complex is important for the restructuring of chromosomes into compact forms allowing them to be easily separated during cell division. SMC2 binds to DNA and aids in the resolution of sister chromatids facilitating the equal distribution of genetic material to daughter cells.

Pathways

SMC2 operates within the cell cycle control pathway where it is involved in mitotic processes. It interacts directly with other components of the cell cycle machinery including Cyclin-dependent kinase 1 (CDK1). SMC2 also plays a role in the DNA repair pathway partnering with proteins like ATM (Ataxia-Telangiectasia Mutated kinase) which senses DNA damage and coordinates repair processes. These pathways highlight the interconnected nature of SMC2's functions in maintaining genetic stability.

SMC2 has been linked to certain cancers due to its pivotal role in ensuring proper mitosis. Disruptions in its function can lead to aneuploidy and genomic instability common features of tumorigenesis. Furthermore SMC2's involvement in DNA repair pathways implies a connection to disorders related to DNA damage like Ataxia-Telangiectasia. This disorder is marked by increased sensitivity to DNA damage and proteins like ATM are key partners of SMC2 in these repair processes. Understanding these links provides insights into potential therapeutic targets for addressing such diseases.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.
See full target information SMC2
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column

Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB10399

Anti-SMC2 antibody

primary-antibodies

smc2-antibody-ab10399

4

(3 reviews)

Alternative Product
Primary Antibodies

AB10412

Anti-SMC2 antibody

primary-antibodies

smc2-antibody-ab10412

4

(1 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com