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AB275963

Anti-SMC3 antibody [BLR099G] - BSA free

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(1 Publication)

Rabbit Recombinant Monoclonal SMC3 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SMC3 aa 1150 to C-terminus.

View Alternative Names

BAM, BMH, CSPG6, SMC3L1, SMC3, Structural maintenance of chromosomes protein 3, SMC protein 3, SMC-3, Basement membrane-associated chondroitin proteoglycan, Chondroitin sulfate proteoglycan 6, Chromosome-associated polypeptide, Bamacan, hCAP

3 Images
Immunoprecipitation - Anti-SMC3 antibody [BLR099G] - BSA free (AB275963)
  • IP

Supplier Data

Immunoprecipitation - Anti-SMC3 antibody [BLR099G] - BSA free (AB275963)

Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. ab275963 used for IP at 6 μl per reaction (Lane 1). For blotting immunoprecipitated SMC3, ab275963 was used at 1/1000 dilution. Lane 3 shows a control IgG.

Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-SMC3 antibody [BLR099G] - BSA free (ab275963)

Predicted band size: 141 kDa

false

Western blot - Anti-SMC3 antibody [BLR099G] - BSA free (AB275963)
  • WB

Supplier Data

Western blot - Anti-SMC3 antibody [BLR099G] - BSA free (AB275963)

Chemiluminescence with an exposure time of 10 seconds.

Lower Panel : Rabbit antiCOPB2 antibody

All lanes:

Western blot - Anti-SMC3 antibody [BLR099G] - BSA free (ab275963) at 1/1000 dilution

Lane 1:

HEK-293T whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

LNCaP whole cell lysate at 50 µg

Lane 5:

A549 whole cell lysate at 50 µg

Lane 6:

MCF-7 whole cell lysate at 50 µg

Lane 7:

GaMG whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 141 kDa

false

Western blot - Anti-SMC3 antibody [BLR099G] - BSA free (AB275963)
  • WB

Supplier Data

Western blot - Anti-SMC3 antibody [BLR099G] - BSA free (AB275963)

Chemiluminescence with an exposure time of 10 seconds.

Lower Panel : Rabbit anti-COPB2 antibody

All lanes:

Western blot - Anti-SMC3 antibody [BLR099G] - BSA free (ab275963) at 1/1000 dilution

Lane 1:

C2C12 whole cell lysate at 50 µg

Lane 2:

70Z/3 whole cell lysate at 50 µg

Lane 3:

BW5147.3 whole cell lysate at 50 µg

Lane 4:

CTLL-2 whole cell lysate at 50 µg

Lane 5:

RenCa whole cell lysate at 50 µg

Lane 6:

NIH/3T3 whole cell lysate at 50 µg

Lane 7:

TCMK-1 whole cell lysate at 50 µg

Lane 8:

A20 whole cell lysate at 50 µg

Lane 9:

Ba/F3 whole cell lysate at 50 µg

Lane 10:

CH27 whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 141 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR099G

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human SMC3 aa 1150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UQE7

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Secondary Antibodies

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  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>6 μl/1 mg lysate</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 8.2 Preservative: 0.09% Sodium azide Constituents: 99.01% Borate buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMC3 also known as Structural Maintenance of Chromosomes 3 is a core component of the cohesin complex playing a vital role in chromosomal architecture and stability. It has a molecular mass of approximately 141 kDa. SMC3 is ubiquitously expressed across various tissues and is notably abundant in proliferating cells. Using an ATPase domain SMC3 helps in the ATP-dependent binding and release of cohesin from chromatin contributing to sister chromatid cohesion during cell division.
Biological function summary

Cohesin complexes rely on SMC3 for the maintenance of chromosome cohesion condensation and repair. The protein is essential for the proper segregation of chromosomes during mitosis and meiosis. SMC3 part of the cohesin complex along with SMC1 RAD21 and SCC3 forms a ring-like structure encircling sister chromatids ensuring they remain attached until anaphase. Besides structural purposes SMC3 facilitates DNA damage response contributing to genome integrity by allowing homologous recombination.

Pathways

SMC3 is integral in the cell cycle regulation and DNA repair pathways. During the cell cycle SMC3 cooperates with SMC1 and RAD21 in the establishment of cohesin networks necessary for genome integrity. In terms of DNA repair SMC3 supports homologous recombination an important mechanism of DNA double-strand break repair. The protein interacts with PLK1 and CDK1 influencing progression through the G2/M checkpoint in response to DNA damage.

SMC3 is linked with Cornelia de Lange syndrome a developmental disorder characterized by growth and cognitive delays. Mutations in SMC3 disrupt its ability to maintain chromosome cohesion leading to defects in DNA replication and repair processes. Additionally SMC3 aberrations associate with cohesinopathies a group of diseases related to mutations in cohesin complex components like NIPBL and SMC1 highlighting SMC3's importance in genetic stability and normal development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex also plays an important role in spindle pole assembly during mitosis and in chromosomes movement.
See full target information SMC3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental physiology 108:961-974 PubMed37139700

2023

High doses of rosuvastatin induce impaired branched-chain amino acid catabolism and lead to insulin resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Bai,Xingzhen Long,Fang Song,Baolin Chen,Changcheng Sheng,Cailin Tang,Li Li,Jiaxing Zhang,Rui Zhang,Jiquan Zhang,Jiali Li
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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