Rabbit Polyclonal SMC3 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SMC3 aa 900-1200.
Images
Publications
- Molecular cell 73:212-223.e72018Repression of Transcription at DNA Breaks Requires Cohesin throughout Interphase and Prevents Genome Instability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCornelia Meisenberg,Sarah I Pinder,Suzanna R Hopkins,Sarah K Wooller,Graeme Benstead-Hume,Frances M G Pearl,Penny A Jeggo,Jessica A DownsPubMed 30554942
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SMC3 aa 900-1200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UQE7
Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Tested | Expected | Expected |
| Chicken | Predicted | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted | Predicted |
| Zebrafish | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Rat | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Xenopus laevis, Zebrafish | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Xenopus laevis, Zebrafish | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Xenopus laevis, Zebrafish | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement.
Alternative names
Structural maintenance of chromosomes protein 3, SMC protein 3, SMC-3, Basement membrane-associated chondroitin proteoglycan, Chondroitin sulfate proteoglycan 6, Chromosome-associated polypeptide, Bamacan, hCAP, BAM, SMC3, SMC3L1, BMH, CSPG6
Recommended products
Rabbit Polyclonal SMC3 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SMC3 aa 900-1200.
Alternative names
Structural maintenance of chromosomes protein 3, SMC protein 3, SMC-3, Basement membrane-associated chondroitin proteoglycan, Chondroitin sulfate proteoglycan 6, Chromosome-associated polypeptide, Bamacan, hCAP, BAM, SMC3, SMC3L1, BMH, CSPG6
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SMC3 aa 900-1200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UQE7
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- Activity summary
SMC3 also known as Structural Maintenance of Chromosomes 3 is a core component of the cohesin complex playing a vital role in chromosomal architecture and stability. It has a molecular mass of approximately 141 kDa. SMC3 is ubiquitously expressed across various tissues and is notably abundant in proliferating cells. Using an ATPase domain SMC3 helps in the ATP-dependent binding and release of cohesin from chromatin contributing to sister chromatid cohesion during cell division.
- Biological function summary
Cohesin complexes rely on SMC3 for the maintenance of chromosome cohesion condensation and repair. The protein is essential for the proper segregation of chromosomes during mitosis and meiosis. SMC3 part of the cohesin complex along with SMC1 RAD21 and SCC3 forms a ring-like structure encircling sister chromatids ensuring they remain attached until anaphase. Besides structural purposes SMC3 facilitates DNA damage response contributing to genome integrity by allowing homologous recombination.
- Pathways
SMC3 is integral in the cell cycle regulation and DNA repair pathways. During the cell cycle SMC3 cooperates with SMC1 and RAD21 in the establishment of cohesin networks necessary for genome integrity. In terms of DNA repair SMC3 supports homologous recombination an important mechanism of DNA double-strand break repair. The protein interacts with PLK1 and CDK1 influencing progression through the G2/M checkpoint in response to DNA damage.
- Associated diseases and disorders
SMC3 is linked with Cornelia de Lange syndrome a developmental disorder characterized by growth and cognitive delays. Mutations in SMC3 disrupt its ability to maintain chromosome cohesion leading to defects in DNA replication and repair processes. Additionally SMC3 aberrations associate with cohesinopathies a group of diseases related to mutations in cohesin complex components like NIPBL and SMC1 highlighting SMC3's importance in genetic stability and normal development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Western blot - Anti-SMC3 antibody - C-terminal (ab155587)
5% SDS PAGE
All lanes: Western blot - Anti-SMC3 antibody - C-terminal (ab155587) at 1/1000 dilution
All lanes: HepG2 whole cell lysate at 30 µg
Predicted band size: 141 kDa
Western blot - Anti-SMC3 antibody - C-terminal (ab155587)
5% SDS PAGE
All lanes: Western blot - Anti-SMC3 antibody - C-terminal (ab155587) at 1/1000 dilution
Lane 1: NIH3T3 whole cell lysate at 30 µg
Lane 2: JC whole cell lysate at 30 µg
Lane 3: BCL-1 whole cell lysate at 30 µg
Predicted band size: 141 kDa
Western blot - Anti-SMC3 antibody - C-terminal (ab155587)
5% SDS PAGE
All lanes: Western blot - Anti-SMC3 antibody - C-terminal (ab155587) at 1/2000 dilution
All lanes: PC12 whole cell lysate at 30 µg
Predicted band size: 141 kDa, 171 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SMC3 antibody - C-terminal (ab155587)
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling SMC3 with ab155587 at 1/200 dilution. Lower panel merged with DNA probe.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC3 antibody - C-terminal (ab155587)
Immunohistochemical analysis of paraffin-embedded Cal27 (human) xenograft tissue labeling SMC3 with ab155587 at 1/100 dilution.
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