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Rabbit Polyclonal SMC3 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SMC3 aa 900-1200.

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Images

Western blot - Anti-SMC3 antibody - C-terminal (AB155587), expandable thumbnail
  • Western blot - Anti-SMC3 antibody - C-terminal (AB155587), expandable thumbnail
  • Western blot - Anti-SMC3 antibody - C-terminal (AB155587), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SMC3 antibody - C-terminal (AB155587), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC3 antibody - C-terminal (AB155587), expandable thumbnail

Publications

  • Molecular cell 73:212-223.e72018
    Repression of Transcription at DNA Breaks Requires Cohesin throughout Interphase and Prevents Genome Instability.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Cornelia Meisenberg,Sarah I Pinder,Suzanna R Hopkins,Sarah K Wooller,Graeme Benstead-Hume,Frances M G Pearl,Penny A Jeggo,Jessica A Downs
    PubMed 30554942

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human SMC3 aa 900-1200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UQE7

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-P
Human
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Rat
Tested
Expected
Expected
Chicken
Predicted
Predicted
Predicted
Xenopus laevis
Predicted
Predicted
Predicted
Zebrafish
Predicted
Predicted
Predicted

Tested
Tested

Species

Mouse

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Species

Rat

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Species

Human

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Predicted
Predicted

Species

Chicken, Xenopus laevis, Zebrafish

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Chicken, Xenopus laevis, Zebrafish

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Chicken, Xenopus laevis, Zebrafish

Dilution info

-

Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement.

Alternative names

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Rabbit Polyclonal SMC3 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SMC3 aa 900-1200.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human SMC3 aa 900-1200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UQE7
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Activity summary

SMC3 also known as Structural Maintenance of Chromosomes 3 is a core component of the cohesin complex playing a vital role in chromosomal architecture and stability. It has a molecular mass of approximately 141 kDa. SMC3 is ubiquitously expressed across various tissues and is notably abundant in proliferating cells. Using an ATPase domain SMC3 helps in the ATP-dependent binding and release of cohesin from chromatin contributing to sister chromatid cohesion during cell division.

Biological function summary

Cohesin complexes rely on SMC3 for the maintenance of chromosome cohesion condensation and repair. The protein is essential for the proper segregation of chromosomes during mitosis and meiosis. SMC3 part of the cohesin complex along with SMC1 RAD21 and SCC3 forms a ring-like structure encircling sister chromatids ensuring they remain attached until anaphase. Besides structural purposes SMC3 facilitates DNA damage response contributing to genome integrity by allowing homologous recombination.

Pathways

SMC3 is integral in the cell cycle regulation and DNA repair pathways. During the cell cycle SMC3 cooperates with SMC1 and RAD21 in the establishment of cohesin networks necessary for genome integrity. In terms of DNA repair SMC3 supports homologous recombination an important mechanism of DNA double-strand break repair. The protein interacts with PLK1 and CDK1 influencing progression through the G2/M checkpoint in response to DNA damage.

Associated diseases and disorders

SMC3 is linked with Cornelia de Lange syndrome a developmental disorder characterized by growth and cognitive delays. Mutations in SMC3 disrupt its ability to maintain chromosome cohesion leading to defects in DNA replication and repair processes. Additionally SMC3 aberrations associate with cohesinopathies a group of diseases related to mutations in cohesin complex components like NIPBL and SMC1 highlighting SMC3's importance in genetic stability and normal development.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-SMC3 antibody - C-terminal (ab155587), expandable thumbnail

    Western blot - Anti-SMC3 antibody - C-terminal (ab155587)

    5% SDS PAGE

    All lanes: Western blot - Anti-SMC3 antibody - C-terminal (ab155587) at 1/1000 dilution

    All lanes: HepG2 whole cell lysate at 30 µg

    Predicted band size: 141 kDa

  • Western blot - Anti-SMC3 antibody - C-terminal (ab155587), expandable thumbnail

    Western blot - Anti-SMC3 antibody - C-terminal (ab155587)

    5% SDS PAGE

    All lanes: Western blot - Anti-SMC3 antibody - C-terminal (ab155587) at 1/1000 dilution

    Lane 1: NIH3T3 whole cell lysate at 30 µg

    Lane 2: JC whole cell lysate at 30 µg

    Lane 3: BCL-1 whole cell lysate at 30 µg

    Predicted band size: 141 kDa

  • Western blot - Anti-SMC3 antibody - C-terminal (ab155587), expandable thumbnail

    Western blot - Anti-SMC3 antibody - C-terminal (ab155587)

    5% SDS PAGE

    All lanes: Western blot - Anti-SMC3 antibody - C-terminal (ab155587) at 1/2000 dilution

    All lanes: PC12 whole cell lysate at 30 µg

    Predicted band size: 141 kDa, 171 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-SMC3 antibody - C-terminal (ab155587), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SMC3 antibody - C-terminal (ab155587)

    Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling SMC3 with ab155587 at 1/200 dilution. Lower panel merged with DNA probe.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC3 antibody - C-terminal (ab155587), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC3 antibody - C-terminal (ab155587)

    Immunohistochemical analysis of paraffin-embedded Cal27 (human) xenograft tissue labeling SMC3 with ab155587 at 1/100 dilution.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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