AB128919

Anti-SMC3 antibody [EPR7984]

RabMAb
Recombinant
What is this?

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(21 Publications)

Rabbit Recombinant Monoclonal SMC3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 21 publications.

View Alternative Names

BAM, BMH, CSPG6, SMC3L1, SMC3, Structural maintenance of chromosomes protein 3, SMC protein 3, SMC-3, Basement membrane-associated chondroitin proteoglycan, Chondroitin sulfate proteoglycan 6, Chromosome-associated polypeptide, Bamacan, hCAP

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC3 antibody [EPR7984] (AB128919)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC3 antibody [EPR7984] (AB128919)

ab128919 at 1/100 dilution staining SMC3 in paraffin-embedded Human colon tissue by immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-SMC3 antibody [EPR7984] (AB128919)

All lanes:

Western blot - Anti-SMC3 antibody [EPR7984] (ab128919) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

A431 cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 141 kDa

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OI-RD Scanning - Anti-SMC3 antibody [EPR7984] (AB128919)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-SMC3 antibody [EPR7984] (AB128919)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-SMC3 antibody [EPR7984]
Carrier free

Anti-SMC3 antibody [EPR7984] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7984

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMC3 also known as Structural Maintenance of Chromosomes 3 is a core component of the cohesin complex playing a vital role in chromosomal architecture and stability. It has a molecular mass of approximately 141 kDa. SMC3 is ubiquitously expressed across various tissues and is notably abundant in proliferating cells. Using an ATPase domain SMC3 helps in the ATP-dependent binding and release of cohesin from chromatin contributing to sister chromatid cohesion during cell division.

Biological function summary

Cohesin complexes rely on SMC3 for the maintenance of chromosome cohesion condensation and repair. The protein is essential for the proper segregation of chromosomes during mitosis and meiosis. SMC3 part of the cohesin complex along with SMC1 RAD21 and SCC3 forms a ring-like structure encircling sister chromatids ensuring they remain attached until anaphase. Besides structural purposes SMC3 facilitates DNA damage response contributing to genome integrity by allowing homologous recombination.

Pathways

SMC3 is integral in the cell cycle regulation and DNA repair pathways. During the cell cycle SMC3 cooperates with SMC1 and RAD21 in the establishment of cohesin networks necessary for genome integrity. In terms of DNA repair SMC3 supports homologous recombination an important mechanism of DNA double-strand break repair. The protein interacts with PLK1 and CDK1 influencing progression through the G2/M checkpoint in response to DNA damage.

SMC3 is linked with Cornelia de Lange syndrome a developmental disorder characterized by growth and cognitive delays. Mutations in SMC3 disrupt its ability to maintain chromosome cohesion leading to defects in DNA replication and repair processes. Additionally SMC3 aberrations associate with cohesinopathies a group of diseases related to mutations in cohesin complex components like NIPBL and SMC1 highlighting SMC3's importance in genetic stability and normal development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex also plays an important role in spindle pole assembly during mitosis and in chromosomes movement.

See full target information SMC3

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Publications (21)

Recent publications for all applications. Explore the full list and refine your search

Genome biology 26:204 PubMed40665375

2025

ZSCAN4 functions as a safeguard to maintain centromere integrity during oocyte meiosis.

Applications

Unspecified application

Species

Unspecified reactive species

Da Yi Choi,Jiyeon Leem,Crystal Lee,Jeong Su Oh

Nature communications 16:5313 PubMed40537528

2025

Herpes simplex virus type 1 reshapes host chromatin architecture via transcription machinery hijacking.

Applications

Unspecified application

Species

Unspecified reactive species

Esther González-Almela,Alvaro Castells-Garcia,François Le Dily,Manuel Fernández Merino,Davide Carnevali,Pol Cusco,Luciano Di Croce,Maria Pia Cosma

Nature communications 15:10713 PubMed39715766

2024

Chromosome architecture and low cohesion bias acrocentric chromosomes towards aneuploidy during mammalian meiosis.

Applications

Unspecified application

Species

Unspecified reactive species

Eirini Bellou,Agata P Zielinska,Eike Urs Mönnich,Nina Schweizer,Antonio Z Politi,Antonina Wellecke,Claus Sibold,Andreas Tandler-Schneider,Melina Schuh

Nucleic acids research 51:4899-4913 PubMed36999590

2023

Oocytes can repair DNA damage during meiosis via a microtubule-dependent recruitment of CIP2A-MDC1-TOPBP1 complex from spindle pole to chromosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Jiyeon Leem,Jae-Sung Kim,Jeong Su Oh

Cell proliferation 56:e13384 PubMed36564861

2022

Selective utilization of non-homologous end-joining and homologous recombination for DNA repair during meiotic maturation in mouse oocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Crystal Lee,Jiyeon Leem,Jeong Su Oh

Nature communications 13:7732 PubMed36513638

2022

CENP-F-dependent DRP1 function regulates APC/C activity during oocyte meiosis I.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng-Jie Zhou,Xing-Yue Wang,Yan-Hua Dong,Dong-Hui Wang,Zhe Han,Xiao-Jie Zhang,Qing-Yuan Sun,John Carroll,Cheng-Guang Liang

Cell cycle (Georgetown, Tex.) 21:1867-1878 PubMed35536551

2022

Knockout of cyclin B1 in granulosa cells causes female subfertility.

Applications

Unspecified application

Species

Unspecified reactive species

Jinmei Cheng,Yixun Liu

Frontiers in cell and developmental biology 9:749116 PubMed34604243

2021

TRF1 Depletion Reveals Mutual Regulation Between Telomeres, Kinetochores, and Inner Centromeres in Mouse Oocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Hyuk-Joon Jeon,Jeong Su Oh

Molecular cell 81:3065-3081.e12 PubMed34297911

2021

Transcription-mediated supercoiling regulates genome folding and loop formation.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Victoria Neguembor,Laura Martin,Álvaro Castells-García,Pablo Aurelio Gómez-García,Chiara Vicario,Davide Carnevali,Jumana AlHaj Abed,Alba Granados,Ruben Sebastian-Perez,Francesco Sottile,Jérôme Solon,Chao-Ting Wu,Melike Lakadamyali,Maria Pia Cosma

Frontiers in cell and developmental biology 9:671685 PubMed34277613

2021

Cell Division Cycle 5-Like Regulates Metaphase-to-Anaphase Transition in Meiotic Oocyte.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Yong Zhang,Jian Li,Ying-Chun Ouyang,Tie-Gang Meng,Chun-Hui Zhang,Wei Yue,Qing-Yuan Sun,Wei-Ping Qian

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SMC3 antibody [EPR7984]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC3 antibody [EPR7984] (AB128919)

Western blot - Anti-SMC3 antibody [EPR7984] (AB128919)

OI-RD Scanning - Anti-SMC3 antibody [EPR7984] (AB128919)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (21)

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