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AB264351

Anti-SMC3 (phospho S1083) antibody

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Rabbit Polyclonal SMC3 phospho S1083 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human SMC3 phospho S1083.

View Alternative Names

BAM, BMH, CSPG6, SMC3L1, SMC3, Structural maintenance of chromosomes protein 3, SMC protein 3, SMC-3, Basement membrane-associated chondroitin proteoglycan, Chondroitin sulfate proteoglycan 6, Chromosome-associated polypeptide, Bamacan, hCAP

2 Images
Western blot - Anti-SMC3 (phospho S1083) antibody (AB264351)
  • WB

Supplier Data

Western blot - Anti-SMC3 (phospho S1083) antibody (AB264351)

Exposure times.

Upper panel : 3 minutes.

Lower panel : 10 seconds.

All lanes:

Western blot - Anti-SMC3 (phospho S1083) antibody (ab264351) at 1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) (treated with 100 uM ectoposide for 4 hours) whole cell lysate at 50 µg

Lane 2:

HeLa (treated with 100 uM ectoposide + 100 uM phosphatase for 4 hours) whole cell lysate at 50 µg

Lane 3:

Untreated HeLa whole cell lysate at 50 µg

Predicted band size: 141 kDa

false

Immunoprecipitation - Anti-SMC3 (phospho S1083) antibody (AB264351)
  • IP

Supplier Data

Immunoprecipitation - Anti-SMC3 (phospho S1083) antibody (AB264351)

SMC3 (phospho S1083) was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264351 at 6 μg per reaction. Western blot was performed on the immunoprecipitates using ab264351 at 1 μg/ml.

Lane 1 : ab264351 IP in HeLa (100 uM ectoposide for 4 hours) whole cell lysate.

Lane 2 : ab264351 IP in HeLa (untreated) whole cell lysate.

Lane 3 : Control IgG IP in Hela (ectoposide for 4 hours) whole cell lysate.

Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-SMC3 (phospho S1083) antibody (ab264351)

Predicted band size: 141 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human SMC3 phospho S1083. The exact immunogen used to generate this antibody is proprietary information.

Q9UQE7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2.00000-10.00000 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500.00000 - 1/2500.00000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMC3 also known as Structural Maintenance of Chromosomes 3 is a core component of the cohesin complex playing a vital role in chromosomal architecture and stability. It has a molecular mass of approximately 141 kDa. SMC3 is ubiquitously expressed across various tissues and is notably abundant in proliferating cells. Using an ATPase domain SMC3 helps in the ATP-dependent binding and release of cohesin from chromatin contributing to sister chromatid cohesion during cell division.
Biological function summary

Cohesin complexes rely on SMC3 for the maintenance of chromosome cohesion condensation and repair. The protein is essential for the proper segregation of chromosomes during mitosis and meiosis. SMC3 part of the cohesin complex along with SMC1 RAD21 and SCC3 forms a ring-like structure encircling sister chromatids ensuring they remain attached until anaphase. Besides structural purposes SMC3 facilitates DNA damage response contributing to genome integrity by allowing homologous recombination.

Pathways

SMC3 is integral in the cell cycle regulation and DNA repair pathways. During the cell cycle SMC3 cooperates with SMC1 and RAD21 in the establishment of cohesin networks necessary for genome integrity. In terms of DNA repair SMC3 supports homologous recombination an important mechanism of DNA double-strand break repair. The protein interacts with PLK1 and CDK1 influencing progression through the G2/M checkpoint in response to DNA damage.

SMC3 is linked with Cornelia de Lange syndrome a developmental disorder characterized by growth and cognitive delays. Mutations in SMC3 disrupt its ability to maintain chromosome cohesion leading to defects in DNA replication and repair processes. Additionally SMC3 aberrations associate with cohesinopathies a group of diseases related to mutations in cohesin complex components like NIPBL and SMC1 highlighting SMC3's importance in genetic stability and normal development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex also plays an important role in spindle pole assembly during mitosis and in chromosomes movement.
See full target information SMC3 phospho S1083
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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